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32 Cards in this Set

  • Front
  • Back

Which one is a database

HOLMES

What was the first murder case solved by DNA

Colin Pitchfork

What is the probability of matching a full profile to a full sibling on the NDNAD

1 in 10,000

Which of these extraction techniques is a specialised technique?

Differential

A semen sample is viewed under a compound microscope using a x40 objective lens. What is the overall magnification

400 times

Which of the following substances is not found in seminal fluid?

Maltose

The presumptive test for blood relies on the action of which enzyme?

Peroxidase

Which of the following statements is not true regarding the Chelex 100 extraction?

All the above statements are correct.

Low template DNA is defined as?

Any sample with less than 200pg of DNA.

Forensic DNA analysis is routinely performed using?

Capillary electrophoresis

In differential extraction the pelleted sperm cells are lysed with the addition of a chemical to disrupt the sulphur bonds in the coating of the spermatozoa. That chemical is called?

Dithiothreitol (DTT)

Genetic profiles may not be loaded for inclusion on the NDNAD if?

They have yielded a partial profile containing allelic information for the six loci used in the old SGM system.

The use of fluorescent dyes enables STR regions to be amplified simultaneously. They dues that were used in SGM plus multiplex system are called?

Fam, Joe, Ned and Rox.

Which of the following statements is correct regarding the use of STR markers in genetic profiling?

They have a narrow allelic range.

The biological technique of taking a sample and multiplying it to make millions of identical copies of the same sample is known as?

Polymerase Chain Reaction.

Blood stains can be best detected on a dark surface by?

Ultraviolet Radiation

The custodian of the NDNAD is the?

Home Office

Measurement of amount of a chemical is not important in which of the following forensic tests?

Chemical nature of glass

Which of the following techniques is not routinely used for the analysis of alcohol in the body?

HPLC

Which of the following is a presumptive test for blood?

Luminol

In the presence of seminal fluid, the reagent alpha napthyl phosphate will produce what colour?

Purple

Low template DNA samples can yield useful DNA profiles as a result of?

All of the above

Alpha amylase may be found in?

All of the above

DNA multiplexes contain?

All of the above

Which of the following is an example of low probative evidence?

Finding semen on the inside of a rape suspects underwear.

DVI - disaster victim identification utilise?

All of the above

Where would you most likely find an STR?

Intron

In Qiagen extraction, the DNA is eluted by?

Washing with water

Which of the following are components of the Christmas tree staining procedure for sperm identification?

Nuclear fast red and acid indigocamine

Which of the following cannot be used in a presumptive test for seminal fluid?

Phenolpthalein

Which one of the following statements is incorrect?

Gas-liquid chromatography uses a liquid as the mobile phase.

35 packages of powder. Of these packages, 6 packages were taken at random and found to contain cocaine. The total weight of the 6 packages was 54.02g and the purity was determined as 37%. The total weight of the pure cocaine in the 35 packages was likely to be?

117g