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5 Cards in this Set
- Front
- Back
- 3rd side (hint)
Giardia lamblia
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STOOL:
Macroscopic: Offensive odour, pale, colourless, fatty and floats in water. Microscopic: Cysts and trophozoites in Saline and Iodine wet mounts. ETEROTEST: String test. SEROLOGICAL TESTS: An (GAS-65): ELISA, IIF. Ab: ELISA, IIF MOLECULAR DIAGNOSIS: DNA probes, PCR. |
Tennis racket, causes diarrhoea.
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Trichomonas vaginalis
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MICRO:
Specimen: vaginal, urethral discharge (in females) urine or prostatic secretions (in males). Saline wet mount: jerky and twitching motility. Fixed smears: Acridine orange, Papnicolau, Giemsa. Direct Fluorescent Antibody(DFA). CULTURE: (gold stand) Cysteine-peptone-liver-maltose (CPLM) medium. Plastic envelope medium. (PEM) SEROLOGY: ELISA MOLECULAR: PCR and DNA hybridisation. |
Strawberry mucosa, female genital tract.
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Tryoansomiasis
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MICRO:
Detection of Trypanosomes in: -Wet mount preps (LN aspirate) -Giemsa (thick peripheral smear) -Wet mount, stained smear of CSF. CULTURE: Weinman's or Tobie's IMAGING: CT: cerebral edema. MRI: White matter enhancement. SERODIAGNOSIS: -Card agglutination trypanosomiasis test (CATT), IHA, IIF, ELISA, CFT. (Ab) -ELISA (An) MOLECULAR: PCR OTHERS: Animal inoculation: White mice. Blood: Anemia, monocytosis, raised ESR, reversed albumin-globulin ratio. |
Trypomastigote (long and short stumpy) and Epimastigite.
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Trypanosoma cruzi
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MICRO:
Buffy coat smear, Fresh anticoagulated blood, wet mount: Mobile trypanosomes. Giemsa Microhematocrit with Acridine orange. CULTURE: Novy, Neal, Nicole medium. ANIMAL INOCULATION: Guinea pig or mice with blood, CSF, LN aspirate. XENODIAGNOSIS: Reduviid bugs reared. H&E: LNs and Skeletal muscles, aspirate from Chagoma: Amastigotes. SEROLOGY: Ab: IHA, CFT(Machado-Guerreiro test), ELISA, IHA, DAT, Chaga's radioimmuno precipitation assay (RIPA) INTRADERMAL: Cruzin MOLECULAR: PCR OTHERS: ECG, Chest X-ray, Endoscopy. |
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Leishmania donovani
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DIRECT:
Demonstration of LD bodies: Stained thick blood smear, splenic, bone marrow, and LN aspirate using Giemsa, Leishman, or Wright's stain. Culture: NNN, or Schneider's drosophila tissue culture medium. Animal inoculation: Chinese golden hamster. INDIRECT: Serodiagnosis: ELISA (An) Ab: CFT with WKK An DAT IFAT CIEP DOT-ELISA ICT using rk39 An. Molecular: PCR, DNA probes. Nonspecific: Aldehyde test, Chopra's antimony test. Skin: Leishmanin or Montenegro. Blood: Anemia, Progressive leucopenia, reverse albumin-goobulin ratio. |
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