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68 Cards in this Set

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mRNA

Messenger RNA: carries information from DNA to ribosome.

tRNA

Transfer RNA: Binds with specific amino acids, carry amino acids to ribosome.

rRNA

Ribosomal RNA: component of ribosomes, enzymatic function.

What is in mRNA?

Has codons to be translated by ribosome.

What does tRNA do?

adaptive molecule:


1. has to read codons.


2. carry appropriate amino acids.

What happens during Initiation of mRNA Synthesis?

Begins at promoter sequence. (TATA Box in eukaryotes)


DNA binds to protein.


DNA unwinds.


Enters at active site, unwound and base pairs are added to RNA strand.

What happens during Elongation of mRNA synthesis?

Rudder and zipper keep moving down the DNA strand, locking RNA molecule into place.


Complementary base pairs are made, antiparallel.


DNA goes out channel of protein and rewinds itself.


mRNA comes out of protein as a single strand.

What happens during Termination on mRNA synthesis?

Happens when terminator sequence in reached. Eukaryotes: polyadenylation signal (AAAAAAAA)

What is primary transcript?

product of transcription.

What does RNA processing produce?

More mature RNA.


What are three ways to make RNA more mature?

1. Adding of 5' cap- 2-methylguanasine


2. Removal of introns (splicing)


3. Adding of 3' poly A tail

What happens in splicing?

snRNPs find introns and remove them, exons then joined together.

What is alternative splicing?

creating a unique combo of exons so that 1 gene can code for more than one protein.


How many different types of tRNA are there?

40!!!!!!!!!!

What enzyme attaches amino acids to tRNA?

aminoacyl tRNA synthase

What are the two parts of the ribosome called?

Large subunit and small subunit.

What is the A site in Translation?

aminoacyl tRNA site- loaded tRNA enters here.

What is the P site in Translation?

Peptiolyl site

What is the E site in Translation?

E site- empty tRNA leave through the E site.

What happens during Initiation in Translation?

Starts with small subunit of ribosome.


Proteins called inititation factors.


mRNA binds to small subunit in ribosome.


-initiator aminoacyl tRNA binds to start codon, creates complementary base pairs with codon.


large subunit is then added to the process.

What happens during Elongation in Translation?

peptide bond formation


tRNA enters at A site


Addition of anticodon puts the amino acids very close to each other


peptide bond forms between the two amino acids


Translocation- ribosome moves codon down, tRNA shifts down as well.

What happens during Termination in Translation?

stop codon


Protein release factor binds to stop codon


empty tRNA is released.


synthesis stops


What is a cell?

basic structural and functional unit of life.

Tissue

group of cells that function as a unit.

Organs

groups of tissue organized into a unit.

Organ system

groups of organs and tissues that work together to perform a function.

Epithelial tissue

continuous layer of cells


cover body surfaces


line body cavities


endothelium- cells that line blood vessels


form glands

Function of epithelial tissue

1. protection


2. absorption


3. secretion (glands) NOT EXCRETION


4. sensation

Two sides to epithelial tissue

apical side (inside)


basolateral side (outside)- connects to other tissues.

Connective Tissue

relatively few cells


intercellular substance (matrix)


Four types of connective tissue

1. Loose: soft, extracellular matrix, provides padding


2. Dense: fibrous, extracellular matrix, provides connections


3. Supporting: form, extracellular matrix, functions in support and protection


4. Fluid: liquid, extracellular matrix, functions in transport

Nervous tissue

cells:


neurons- transmit electrical signals


glial cells- supporting cells


Muscle Tissue

moves body


pumps the heart


moving and mixing of food in digestive system

Three types of muscle tissue

Skeletal- attaches to bones


Cardiac- walls of the heart


Smooth- walls of digestive organs and blood vessels

Homeostasis

stability in chemical and physical conditions in n organism's body.

Internal conditions maintained by homeostasis

temperature


blood pressure


blood pH


blood glucose

What are three parts of the Regulatory system?

Sensor- sense the environment


Integrator- evaluates information, decids on action


Effector- carries out response


ex. hormonal regulation of blood glucose

Electrolyte

solute that ionizes in water, contribute to electrical signals


Ex. Na+, K+, Ca2+, Cl-

osmolarity

concentration of dissolved substances in a solution

isosmotic

equal solute concentration

hyperosmotic

higher solute concentration

hyposmotic

lower solute concentration

Passive transport

based on concentration gradient


Ex. direct diffusion


channel proteins


carrier proteins

active transport

requires an energy source to move molecules against a gradient

Primary and secondary active transport

1. Sodium potassium pump- driven by energy from ATP


2. symporters, antiporter- driven by energy from gradient

Cotransport

Uses energy stored in an electrochemical gradient to transport a different solute against its concentration gradient.

Symport

solutes move in the same direction

antiport

solutes move in the opposite direction

The nephron

functional unit of the kidney

Neprhon functions

1. filtration- size selective


2. reabsorption- things added back into the blood


3. secretion


4. excretion- urine!

filtration

blood is filtered from glomerulus capillaries into Bowman's capsule

reabsorption

products being added back into the blood


secretion

continual secretion of substances into the tubular fluid, enhances the kidneys ability to eliminate certain wastes and toxins.

excretion

what goes into the urine, releasing of urine into the toilet!!!

Composition of filtrate

water


nutrients (glucose, amino acids)


wastes (urea, drugs)


electrolytes (Na+, K+, Cl-, etc)

Ingesting

taking in food

Digestion

breakdown of food into monomers

Absorption

uptake of nutrients

Elimination

disposal of wastes

Nutrients

proteins


carbohydrates


fats

Essential nutrients

some amino acids


vitamins


minerals


electrolytes

Digestion

chewing increases surface area


saliva lubricates food


enzymes begin to digest food- salivary amylase, lingual lipase (fat digestion)


Esophagus

muscle constricts, muscle moves food


muscle contraction moves in waves down esophagus, pushing food below to stomach

Stomach

muscle contractions mix contents


low pH due to HCl


Pepsin digests proteins

Small intestine

proteins, carbs and lipids are broken down by pancreas


monomer are transported into epithelial cells


transport is selective and active

Large Intestine

solid waste compacted into feces


additional water absorption occurs


normal microbiota digest cellulose and produce nutrients like Vitamin K

appendix

contains immune tissue


harbors symbiotic bacteria

anus

waster is eliminated through the anus