Exercise 24,25,26,27,28,29,30

Front 1

24#Oxidase Test
What does it do

Back 1

Differentiate Gram negative aerobic microaerophilic rods and cocci from gram negative faculative anaerobic rods

Front 2

Oxidase Test
Test results

Back 2

Blue color: Oxidase positive
No blue color: Oxidase negative

Front 3

Oxidase Test
What Enzyme does it use

Back 3

Cytochrome c oxidase

Front 4

25#Nitrate Reduction
What does it do

Back 4

Reduces Nitrate into Nitrite

Front 5

Nitrate reduction
Test results

Back 5

Turns Red: Positive for nitrite reduction
No color change: Negative for nitrite reduction (Add Zinc)
Turns Red(WZinc): Reduced to Nitrite by zinc dust
No color change(WZinc): Negative for reduction beyond the nitrite stage

Front 6

Nitrate reduction
What enzyme does it use

Back 6

Nitrate

Front 7

Anaerobic respiration

Back 7

The process of generating energy by the oxidation of nutrients and using an external electron acceptor other than oxygen.

Front 8

26#Mio:Decarboxylation
What does it do

Back 8

Contains Glucose for bacteria to ferment into acid. Ph indicators turns MIO agar colors

Front 9

Mio:Decarboxylation
Test Results

Back 9

Purple at top & cloudy throughout: Positive for amino acid being decarbocylated into putrescine
Purple at top & bright yellow below: Negative, organism lacks decarboxylase enzyme
Bright yellow throughout: Negative, Glucose fermented to acid but organism lacks decarboxylase
(WKovac's)Cherry Red layer: Positive, Tryptophan is broken down to indole
Yellow layer: Negative, Tryptophan not broken down to Indole.

Motility: Away from stab line motile, grown in stab line not motile.

Front 10

Mio:Decarboxylation
What Enzyme does it use

Back 10

Decarboxylase

Front 11

Fermintation

Back 11

Fermentation is a metabolic process that converts sugar to acids, gases and/or alcohol.

Front 12

#27Phenylalanine deamination
What does it do

Back 12

Differentiates Proteus from other members of the family Enterobacteriacae.

Front 13

#27Phenylalanine deamination
Test results

Back 13

Green: Positive, organism has phenylalanine deaminase
Yellow: Negative, organism lacks phenylalanine deaminase

Front 14

#27Phenylalanine deamination
Enzyme used

Back 14

Deaminase

Front 15

#27Phenylalanine deamination
Media used

Back 15

Phenylalanine media

Front 16

28#Phenol Red Carbohydrate Fermentation
What does it do

Back 16

Detects Ph levels by fermenting carbohydrates within the agar

Front 17

28#Phenol Red Carbohydrate Fermentation
Media used

Back 17

PR Glucose broth
PR Sucrose broth
PR Lactose broth

Front 18

28#Phenol Red Carbohydrate Fermentation
Test results

Back 18

Yellow: Positive for carbohydrate fermentation
If Air bubbles: Positive for gas formation
Pinker than control: Negative for carbohydrate fermentation (Alkaline/K)

Front 19

28#Phenol Red Carbohydrate Fermentation
Ingredients used

Back 19

Glucose, Sucrose, Lactose

Front 20

29#Triple Sugar Iron Test
What does it do

Back 20

Ferments sugar into acid. Agar contains 3 different sugars. Glucose, Lactose, Sucrose

Front 21

29#Triple Sugar Iron Test
What agar is used

Back 21

TSI Agar Slants

Front 22

29#Triple Sugar Iron Test
Ingredients used

Back 22

Glucose, Lactose, Sucrose

Front 23

29#Triple Sugar Iron Test
Results

Back 23

Yellow slant/Yellow butt: G,L, and S fermented to acid(A/A)
Pink Slant/Yellow butt: G fermented to acid and protein, broken down into alkaline.(K/A)
Pink slant/Pink butt: No sugars fermented(K/K)
Pink slant/Neutral butt: No sugars fermented.(K/-) Protein aerobically converted to alkaline.
Neutral slant/Neautral butt: Organism failed to grow(-/-)
Blackening along stab line: Hydrogen sulfide gas production(H2S)
Bubbles or breaks in agar no black: Build up of gas other than hydrogen sulfide. (G)

Front 24

30#Urease Test
What does it do

Back 24

Converts Urea into ammonia and CO2 gas. Ammonia turns the pH indicator bright pink which is positive for Urease enzyme

Front 25

30#Urease Test
What enzyme does it use

Back 25

Urease

Front 26

30#Urease Test
Test results

Back 26

Light Pink: Hydrolysis
No change/Negative control: Organism lacks Urease enzyme

Front 27

30#Urease Test
What agar is used

Back 27

Urea Broth

Front 28

What is this and what is positive

Back 28

Oxidase Test, blue color

Front 29

What is this and what is positive

Back 29

Citrate, blue color

Front 30

What is this and what is positive

Back 30

Phenylalanine media
Green: Positive, organism has phenylalanine deaminase

Front 31

What is this and what do the colors mean

Back 31

Triple Sugar Slants
Yellow: Fermented to acid
Pink: Not fermented to acid
Neutral: Organism failed to grow
Blackening: Hydrogen sulfide gas produced
Bubbles/Breaks: Gas build up other than Hydrogen Sulfide

Front 32

What is this which is positive

Back 32

Urea broth
Pink: Hydrolysis

Front 33

Hydrolysis

Back 33

Breaking of a bond in a molecule using water

Front 34

What is this what do colors mean

Back 34

Mio Agar: Decarboxylation
Purple at top & cloudy throughout: Positive for amino acid being decarbocylated into putrescine
Purple at top & bright yellow below: Negative, organism lacks decarboxylase enzyme
Bright yellow throughout: Negative, Glucose fermented to acid but organism lacks decarboxylase
(WKovac's)Cherry Red layer: Positive, Tryptophan is broken down to indole
Yellow layer: Negative, Tryptophan not broken down to Indole.

Motility: Away from stab line motile, grown in stab line not motile.

Front 35

What is this what colors are positive

Back 35

Nitrate broth
Red: Positive for nitrate
No color change(After Zinc): Positive for nitrate reduction beyond nitrite stage

Front 36

What is this what do colors mean what does air bubble mean

Back 36

PR Glucose broth
PR Sucrose broth
PR Lactose broth
Yellow: Positive for carbohydrate fermentaiton
Bubble: Gas production