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11 Cards in this Set
- Front
- Back
What type of staining does Mycobacterium show? (Acid-fast/ non acid-fast) |
Acid-Fast
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Why is Escherichia coli stained blue in Acid-fast Staining and not red as in Gram Staining?
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Escherichia coli is a NON ACID-FAST bacterium.
(1) Bacteria are DECOLORIZES by ACID ALCOHOL and DO NOT retain the initial stain, carbolfuchsin, (2) so it can pick up the counterstain, METHYLENE BLUE. That is why it is stained blue instead of red. |
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What is the purpose of this staining?
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To distinguish between acid-fast and non acid-fast bacteria. |
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Name two genera of acid-fast bacteria.
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Mycobacterium, Nocardia
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Name two important diseases caused by acid-fast bacteria.
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Tuberculosis, leprosy |
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What is Acid-Fast staining?
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Acid fast staining technique is an important diagnostic procedure for identification of pathogenic members of Mycobacteria.
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What is Ziehl-Neelson method?
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Ziehl-Neelson method - most widely used procedure for acid fast staining.
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The primary stain of acid fast staining is ________________
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The primary stain of acid fast staining is carbolfuchsin
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The counterstain of acid fast staining is ________________
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The counterstain of acid fast staining is methylene blue.
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What is the difference between Acid-Fast and Non Acid-Fast?
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Acid-fast vs. Non acid-fast – |
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What type of bacteria does this stain bind to?
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Bacteria that have a wax-like lipid, MYCOLIC ACID in their cell wall. |
