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18 Cards in this Set
- Front
- Back
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1. Oxidoreductases 2. Transferases 3. Hydrolases 4. Lyases 5. Isomerases 6. Ligases (synthetases) |
1. Ared + Box ⇄ Aox + Bred 2. A -B + C → A + C - B 3. A -B + H 2 O → A - H + B -OH 4. A-B ⇄ A + B (reverse reaction: synthases) 5. A-B-C ⇄ A-C-B 6. A + B + ATP → A-B + ADP + Pi |
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6 types of enzymes |
1. Oxidoreductases 2. Transferases 3. Hydrolases 4. Lyases 5. Isomerases 6. Ligases (synthetases) |
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dehydrogenases oxygenases oxidases peroxidases |
Oxidoreductases |
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aminotransferases, methyltransferases, glucosyltransferases kinases DNA-polymerase |
Transferases |
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esterases (phosphatases) glycosidases proteinases ,peptidases amidases ATPases nucleases |
Hydrolases |
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ammonia lyases decarboxylases aldolases (de)hydratases |
Lyases
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epimerases, racemases, mutases topoisomerase |
Isomerases |
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carboxylases synthetases DNA-ligase |
Ligase |
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Metalloenzymes |
Mo, Zn, Fe, Cu, Mn, Se |
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Factors determining the rate of enzymatic reaction
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Temperature pH activators Inhibitors Substrate consentration |
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Mo |
Xanthine oxidase Sulfite oxidase |
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Zn |
Alcohol dehydrogenase Carbonic anhydrase Carboxypeptidases Cu, Zn-superoxide dismutase |
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Cu |
Ceruloplasmin Cytochrome-c-oxidase Monoamine oxidases Dopamine hydroxylase Lysyl oxidase |
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Mn |
Mn-superoxide dismutase Arginase |
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Fe |
Catalase
Myeloperoxidase Cytochromes |
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Se |
Glutathione peroxidase
Deiodases of thyronines Thioredoxin reductase |
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two ways of determing enzyme quantity |
catalytic concentration µkat/l the product of enzyme reaction is determined mass concentration µg/l enzyme itself is determined as antigen(immunochemical assay) |
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Catalytic activity Catalytic concentration |
kat = mol/s
kat/l = mol/l.s |
