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20 Cards in this Set

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An enzyme's _______________ shape creates an _____________ site.
3 degree (titary)
Active
Enzymes are ___________ to their substrates.
specific
Enzymes are found in ________ things.
living
Enzymes are composed of_____________
amino acids
Enzymes are _______________ catalysts
organic
Enzymes are ________ used up in the reaction.
not
Simple metabolic pathway (chemical reaction)
substrate >----enzyme-----> Product(s)
Substrates:
ONPG (analog)=
starch + Lactose
Substrate: Starch (gives a _________ test)
positive
Enzymes: o-nitrophenol (yellow compound; visual test and test for absorbance with a________________
spectrophotometer
Product: galactose (a ______________)
monosacraride (simple sugar)
Product: glucose (gives a _____________ iodine test)
negitive (brown color)
Buffer:________ enzyme so this is our experimental__________
NO enzyme so this is our experimental CONTROL
ONPG + H2O --------> o-nitrolpheno + galactose what is the enzyme?
beta galactosidase
Starch + H20 -------> glucose + glucose + glucose (many glucose)what is the enzyme?
diastase
Most enzymes end with the suffix______
-ase
Ezymes: ___________ and b-galactosidase
diastase
Increasing the concentrations of the enzyme will make the reaction proceed _________
faster
A spectrophotometer measures the light_________ of a substance
absorbance
The more molecules of o-nitrolphenol the greater the______________
absorbance