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3 Cards in this Set
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What are the two types of probes? What are they made of? |
• Radioactively labelled - using P(32) which can be identified using x-ray photography • fluorescent - a DNA probe that will fluoresce under certain conditions • They are short single-strands of DNA that are easily identified |
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What are DNA probes used for? |
To identify specific alleles of a gene by joining onto its complimentary bases |
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What are the different steps? |
• DNA probe that is complimentary to the desired allele is added • DNA that a being tested is treated to seperate the two stands • Seperate strands are mixed with the DNA probe which will bind to its complimentary base sequence - called DNA hybridisation • Binding site of probe identified |
