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What are the two types of probes?
What are they made of?
• Radioactively labelled - using P(32) which can be identified using x-ray photography
• fluorescent - a DNA probe that will fluoresce under certain conditions
• They are short single-strands of DNA that are easily identified
What are DNA probes used for?
To identify specific alleles of a gene by joining onto its complimentary bases
What are the different steps?
• DNA probe that is complimentary to the desired allele is added
• DNA that a being tested is treated to seperate the two stands
• Seperate strands are mixed with the DNA probe which will bind to its complimentary base sequence - called DNA hybridisation
• Binding site of probe identified
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