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5 Cards in this Set
- Front
- Back
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rRNA |
Ribosomal RNA. Structural component of ribosome for protein synthesis. Makes up 80% of all RNA. Found in eu and prokaryotes |
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tRNA |
Transfer RNA, carries amino acids to ribosomes for translation. Smallet RNA. Makes up 15% of them. Amino Acids bind to 3'end. Anticodon found in 5' end. |
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Analytical techniques used for DNA and RNA analysis |
Hyperchromic Shift DNA that is heated in solution causes increase in UV absorbtion(optical density) Denaturation used to get melting temp. Graph of absorb. And temp guves melting point lf DNA. Tm, means half of DNA base pairs are denatured. The more GC base pairs the higher the meltin point
Molecular Hybridization By raising temp, denaturing occurs Then decreasin temp, annealin of complementary strands Microarray tech used to study extent to which certain genes are turned on or off in cells and tissues. Each spot on dna chio contains dna sequence. Detects gene expression. Uses complementary strand done by hybridization Flouresence In Situ Hybrid. Use probe strand and strand labeled with flourescent dya and then denature and hybridize, creates chromosome painting. Can observe transclocation easier |
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3 theories of NA Replication |
Semiconservative , each replicated DNA consists of old and new strand. Conservative- 2 new strand synthesize together, old strand stays intact. Dispersive-parental strand dispersed into 2 new double helices |
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Meselson Stahl Experim |
Heavy 15N labeled ecoli grow kn light 14N meduim Each new DNA molecule consists of one old and one newly synthesized strand Proved semiconservative in prokaryotes |
