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28 Cards in this Set

  • Front
  • Back

Before materials are disinfected or sterilized, they must be

Cleaned (usually with detergents or enzymes)

Cleaning removes how many folds of bacterial load

1000 folds or more

Disinfection doesn't remove

bacterial spores

The 2 types of disinfection are

• Physical (thermal) disinfection


Chemical disinfection

Thermal(physical) disinfection is grouped into two:

(a) Boiling (at 100 °C for 20 minutes).


(b) Pasteurization ( > 70 °C for 30 minutes): for respiratory and anaesthetic instrument

The levels of disinfection are

(a) High level disinfection ( destroys all microorganisms except bacterial spores).


(b) Intermediate disinfection (destroys Mycobacterium tuberculosis, some viruses , fungi).


(c) Low level disinfection ( destroys some bacteria, fungi, viruses but not Mycobacterium tuberculosis that is a resistant bacteria)

Chemicals used for high level disinfection include

• Sodium hypochlorite (also used for intermediate disinfection)


• Peracetic acid


• Formaldehyde


• Glutaraldehyde


• 6% Hydrogen peroxide


Intermediate level disinfection is done with

(a) Alcohol(b) Iodine(c) Sodium hypochlorite

Hypochlorite unique features

(a) Unaffected by water hardness(b) Rapid acting like alcohol(c) It is corrosive to metals (unlike alcohol)(d) Inactivated by organic matter(e) Discolours fabric (e.g hypo; unlike alcohol)(f) Do not leave toxic residues


Hypochlorite need to be prepared freshly for use because

They are unstable and after preparation they should be used within 24 hours

Duration of application of hypochlorite is

Maximum of 10 minutes

What concentration of hypochlorite is used for High level disinfection ?

• 0.5-1%

What concentration of hypochlorite is used for Low level disinfection ?

0.05%-0.1%

Iodine is used majorly as

Antiseptic

Glutaraldehyde is used for 2 things. Mention them

(a) High level disinfection(b) chemical sterilization

Mention the type of sterilization

Mnemonic : D.E.P.H.I.S


• Dry heat


• Ethylene oxide


• Peracetic acid


• Hydrogen peroxide gas plasma


• Irradiation


• Steam (with autoclave)

The most efficient type of sterilization is

Steam sterilization

Ethylene oxide (ETO) sterilizatioin is used for :

Heats labile materials ( It is flammable, colourless and toxic)

Spaulding classification of materials is into 3 groups . Mention them

(a) Critical items(b) Semi-critical items(c) Non-critical items

What are critical items

These are equipments make direct contact with body's sterile tissues and organs . They penetrate the skin e.g needles and catheters

Critical items are treated with which process

Sterilization strictly (even high disinfection can't be used because bacteria spores will still be present)

What are semicritical items

These are instrument that make contact with non- intact skin and mucous membranes of the body ( e.g mouth, nose, vagina, anus etc)

Semi critical items are treated by what process

Minimum of High-level sterilization (because of resistant microbes that can easily cross mucous membrane)

What are non-critical items ?

These are instruments that make contact with intact skin so they have very low chance of causing infections e.g thermometer, blood pressure cuff etc .

Non-critical items are treated with

Cleaning or low level disinfection would suffice

How often should one clean

Once a day

Environmental cleaning should involve

(a) No sweeping (dust particle transfer microbes.Vacuum is better) (b) No dry dusting (only damp dusting)(c) Damp mopping(d) Use clean water and soap(e) clean from clean area to dirty area

How do you take care of spills

• cover spill area with paper towel or cloth• Pour 0.5-1% bleach and leave for 30 minutes (to sterilize)• Remove towel wltn gloves and discard appropriately• Wash surface with soap and water• Sterilize with 0.5-1% bleach on surface