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150 Cards in this Set

  • Front
  • Back

Bacteria are grouped according to their

-Morphological and metabolic/biochemical differences
How are bacteria classified
Immunologic and genetic characteristic
Gram staining allows the clinician to
Determine shape of organism (round, rod, ect)
Why is gram staining necessary
Most bacteria are colorless and invisible to light microscopy
Gram stain separates organisms into 2 groups:
-gram + (blue)
-gram - (red)
Different gram stains are a result of differences in the
Cell walls
Gram + cell wall:
-very thick
-contain teichoic acid
-does not block diffusion of antibiotics (vulnerable to penicillins and lysozyme)
- has 2 layers
-low lipid content
-no peril admit space/porin channel
-no endotoxin

Gram - cell wall:
-very thin
-cell wall had murein lipoprotein
-contains 3 layers
-high lipid content
-had peri plastic and porin channel (resistant to breakdown by antibiotic)
-outer cell membrane contains edotoxin (lipid A) lipidopolysaccharide
-design allows cell to block diffusion of substances that attack cell walls such as penicillins and lysozyme
Not all organisms are susceptible to gram staining and therefore require specific considerations.

-both statements true
-both statements false
-first true second false
-first false second true
Both statements true
Gram + cocci are divided into 3 groups based on their ability to
Hemolysis RBC's
3 groups if gram + cocci
- Beta hemolytic streptococcus (completely lose RBC's)
- Alpha hemolytic streptococcus
(Partially lose RBC's)
- Gamma hemolytic streptococcus (unable to lose RBC's)
Beta hemolytic streptococcus hemolytic about is due to the production of
Hemolysins
Beta hemolytic streptococcus are arranged in groups
A-U (known as lance field groups)
Which beta hemolytic streptococcus group is the most important human pathogens
A
Many beta streptococcus stains are

Anti-phagocytic

S. Pyrogens produces _____ that dissolves clots making further invasion possible and ____ (spreading factor)
-streptokinase
-hyaluronidase
Hyaluronidase is necessary for ____ tissues during spread of infections
Hyaluronidase
Group A have the ability to adhere to ____ epithelium
Pharyngeal epitherlium
Group A streptococci are causative for maladies such as:
-streptococcal pharyngitis
-Ptolemaic infections
-tonsillitis
-scarlet fever/ rheumatic fever
What two organisms are not susceptible to gram staining in the dental deild

-Myobacteria
-Spirochettes

Inflammatory disease that may develop after an infection with streptococcus bacteria(such as strep throat or scarlet fever) and can involve the heart,joints, skin, and brain
Rheumatic fever
The causative organism for scarlet fever and rheumatic fever is
Streptococcus
Viridans group of streptococcus
-big heterogenous group of streptococcus
-no lance field group
-not bile soluble
-greenish tint when cultured
-alpha hemolytic

Myobacterium require a ____ stain
Acid fast

Tuberculosis is a disease caused by the bacteria called

Mycobacterium Tuberculosis
Tuberculosis is a _____ infection
Bacterial

Tuberculosis is acquired via ______ transmission

Airborne
Spirochettes (tripomema dentricola) require __________ microscopy
Dark feild
Most disease causing microorganisms are
- gram - rods
- gram + oleo morphia bacteria

Gram + cocci

- non mobile
- do NOT form spores

Streptococcus have spherical gram ______ cocci usually arranged in _____ or ______

+


chains or pairs

All _____ are catalase negative

streptococcus

streptococcus are divided into 3 groups based on their ability to _______

hemolyse RBC's

Beta-hemolytic streptococcus ____ lyse RBC's

completely

Alpha-hemolytic streptococcus ____ lyse RBC's

partially

Gamma-hemolytic streptoccocus ____lyse RBC's

unable

What are the 3 groups of streptococcus

-Beta hemolytic


-Alpha hemolytic


-Gamma hemolytic



*think of BAG a RBC

what streptococcus has the hemolytic ability is due to production of hemolysins

beta bemolytic

what streptoccous arranged in group of A-U (known as lancefeild group)

beta hemolytic

Group ____ are among the most important human pathogens

A

Many strains of ______ streptococcus are anti-phagocytic

beta

S. pyrogens produces ______ and ______.

streptokinase and hyaluronidase

dissolves clots making further invasion possible

streptokinase

"spreading factor"; necessary for invading tissue during spread of infections

hyaluronidase

Partially due to their ability to adhere to pharyngeal epithelium group ____ streptcocci are causative for many maladies

A

Group A streptococci are causative for what maladies

-streptoccal pharyngitis


-pyogenic infection


-tonsillitis


-scarlet fever


rheumatic fever

inflammatory disease that may develop after an infection with stretococcus bacteria (such as strep throat or scarlet fever) and can involve the hear, joints, skin, and brain

rheumatic fever

causative organism for scarlet fever and rheumatic fever is ______

streptococcus

Big, heterogeneous group of streptococcus

viridans group

what group of streptococcus has no lancefield group

viridans group

what group of streptococcus is not bile soluble

viridans group

viridis is latin for

green

organisms that produces greenish tint when cultured

viridis

most of the ___ group is alpha hemolytic

viridans

normal inhabitants of nasopharynx and gingival crevices is what group of streptococcus

viridans

viridans group of streptococcus cause what 3 main types of infections

-dental infections


-endocarditis


-abscesses

____ bind to teeth by producing dextran polysaccharides in the presence of sucrose (also known as glycans) can produce acid and dental caries.

s. mutans

_____ group produces acid and dental caries

viridans

S. mutans metabolizes ____ to ____ which creates an acidi

sucrose


lactic acid

Sucrose is also used by S. mutans to produce _______

dextrans

only sugar that can be utilized to form dextrans

sucrose

allows strep to adhere to the tooth and add to the volume of plaque

dextrans

sugar besides sucrose that can be digested by S. mutans

glucose


fructose


lactose

when glucose, fructose, and lactose are digested by S. mutans the end product is

lactic acid

the combination of ____ and _____ leads to dental caries

plaque and acid

S. mutans is only involved in ____ not ______

caries process


perio

dental manipulations can release ____ into the bloodstream and cause endocarditis

viridans

viridans in the bloodstream will implant on the _____ surface of the heart (most commonly damaged heart valves) by producing extracellular _____

enocardial


dextran

subgroup of viridans variety known as ______ and ______ are associated with dental, brain and abdominal abscesses

S. intermedius


S. anginosus

normal inhabitants of the GI tract

S. anginosus

most common viridans organisms

*s. mutans


*s. salivarius


s. mitis


s. sobrinus


s. sanguis


pit and fissure caires

s. mutans

smooth surface caires

s. sobrinus

colonizes oral tissue; associated with the tongue

s. salivarius

endocarditis caused by what viridans organism

s. mitis

plaque colonization and endocarditis

s. sanguis

spherical, gram + bacteria which colonize the mouth and URT of humans a few hours after birth

streptococcus salivarius

Most common aerobic organisms implicated in facial cellulitis

viridians streptococcus

can be encapsulated with polysaccharide layer which makes it very resistant and destructive

streptococcus pneumoniae

generally harmless component of normal flora

staphyloccus

has powerful arsenal of enzymatic and exotoxin weapons

staphylococcus

True or false: staphylococcus is penicillin-G resistant streptococcus is not

True

Important characteristics of staphylococcus

-non mobile


-non spore forming


-spherical gram + cocci arranged in grapelike clusters


-facultative aerobe


-typical lesion of staphylococcus is abscess formation

typical lesion of a staphylococcus is ____ formation

abscess

significant exotoxins of staphylococcus aureus are _____ and ____

coagulase and hyaluronidase

what exotoxins of staphylococcus aureus degrades hyaluronic acid

hyaluronidase

what is the gorund substance of subcutaneous tissue

hyaluronic acid

staphylococcus aureus produces a pigmented compounds called

carotenoids

staphylococcus aureus is a major producer of proteins called

leukocidins

leukocidins destroy

phagocytes

destroyed phagocytes make up much of the material of

pus

what is the most resistant disease causing organism

MRSA (methicillin resistant staphylococcus aureus)

What are the 4 examples of gram + rods (staphylococcus aureus)

-Bacillus


-Clostridium


-Corynebacterium


-Listeria

causative organism for anthrax and food poisoning

bacillus


(gram + rod)

causative organism for tetanus, botulism, gangrene, food poisoning, and antibiotic-resistant pseudomemnranous colitis

clostridium

causative organism for diptheria

corynebacterium

has endotoxin; causative organism for meningitis and sepesis

listeria

causes lockjaw or tentanus; normally inhabits superficial layers of soil; spores are genereally DUST BORN

clostridum tentani

Gram - Cocci

neisseria

causative organism for meningitis and gonorrhea

neisseria

what are the gram - spiral shaped organisms

-spirochetes


what is found in association with NUP/NUG

grap - spiral shaped organisms (spirochetes)

causative organism for syphilis

spirochetes:


-treponemtosies


-lymedisease


-leptospirosis

can derive lactic acid from glucose which creates and acidic environment; considered aciduric

lactobacillus

______ does not produce dextran but produces a different extra polysaccharide called

lactobacillus; lexan

associated with advanced carious lesion deep in enamel and are aciduric

lactobacillus

gram positive pleomorphic rods which form a large proportion of oral microflora

actinomyces

what are the 3 actinomyces

-A. naeslundii


-A. viscosus


-A. odontolyticus

have been implicated in root caries although their role in dental caries initiation and progression is not well understood

actinomyces

morphology of cocci

spherical


morphology of bacilli

rods

morphology of spiral forms

comma shaped


s shaped


spiral shaped

morphology of pleomorphicl

acking distinct shape

growth phases of bacteria refer to ______ of cells not _____ cells

populations


individual

microbes exhibit 4 distinct phases of growth

lag phase


logarithmic


stationary phase


death phase

what growth phase of bacteria is slow at first as bacteria acclimate to their environment

lag phase

_________ activity was not increase during hte lag pahse

cellular metabolic

what growth phase of bacteria: growth occurs exponentially (very rapidly)

logarithmic (exponential) phase

what growth phase of bacteria: microorgansims are most prone to physical and chemical agents

logarithmic (exponential) phase

what growth phase of bacteria: no net increase or decrease in cell numbers as nutrients are used up and waster products build up

stationary phase

what growth phase of bacteria: after the stationary phase ends, microbes die off

death phase

metabolic characteristics of bacteria can be classified based on what 2 factors

oxygen metabolism


carbon and energy sources

what is the most commonly used source of energy and carbon fro humans and bacteria is

glucose

molecular oxygen is (highly or not) reactive

highly

molecular oxygen can form what 3 things

hydrogen peroxide


superoxide radicals


hydroxyl radicals

bacteria posses 3 enzymes to break down oxygen products

catalase


peroxidase


superoxide disumtase

breaks down hydrogen peroxide

catalase and peroxidase

breaks down superoxide radicals

superoxide dismutase

what are the 4 bacteria categories based on oxygen metabolism

obligate aerobes


facultative anaerobes


microaerophilic


obligate anaerobes

what do glycoproteins make in the oral cavity

aquired pellicle

humans are what type of aerobes

obligate aerobes

what metabolic bacteria category have all the enzymes ( catalase, peroxidase, superoxide simutase)

obligate aerobes

what are 4 examples of obligate aerobes

neisseria


pseudomonas


legionella


mycobacterium

what metabolic bacteria category is aerobic (use oxygen)

facultative anaerobes

what metabolic bacteria category can grown in the absence of oxygen by using fermentation

facultative anaerobes

what are 4 examples of faculatative anaerobes

staphylocoecus


listeria


aetinomyces


most other gram - rods

what metabolic bacteria category are aeortolerant aneorbes

microaerophilic bacteria

what metabolic bacteria category uses fermentaion

facultative anaerobes


microaerophilic bacteria


what metabolic bacteria category can tolerate low amounts of oxygen because they have superoxide dismutase

microaerophilic bacteria

what are 4 examples of microaerophilic bacteria

streptococcus


spirochetes


camphylobacter


denitcola

what microaerophilic bacteria is implicated in pregnancy gingivitis

camphylocater

what metabolic bacteria category hate oxygen

obligate anaerobes

what are the 2 examples of obligate anerobes

prevotella


porphyromonas gingivalis (Mr. perio)

oblique fibers make up the _____

PDL

most often associated with hormonal (pregnancy) gingivitis, facial cellulitis, and NUP/NUG

prevotella

possesses an armamentarium of cell surface associated and extracellular activities which are studies intensively for their virulence potential

porphyromonas gingivalis

surface fimbrae allow porphyromaonas gingivalis to adhere to _______ and _______

epithelial and tooth surfaces

porphyromanas gingivalis is a very imortant pathogen for

periodontal diseases

enzyme capable of breaking down collagen in connective tissue and periodontal attachment appartatus

collagenase

porphyromanas gingivalis produces

collagenase