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150 Cards in this Set
- Front
- Back
Bacteria are grouped according to their |
-Morphological and metabolic/biochemical differences
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How are bacteria classified
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Immunologic and genetic characteristic
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Gram staining allows the clinician to
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Determine shape of organism (round, rod, ect)
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Why is gram staining necessary
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Most bacteria are colorless and invisible to light microscopy
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Gram stain separates organisms into 2 groups:
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-gram + (blue)
-gram - (red) |
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Different gram stains are a result of differences in the
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Cell walls
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Gram + cell wall:
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-very thick
-contain teichoic acid -does not block diffusion of antibiotics (vulnerable to penicillins and lysozyme) - has 2 layers -low lipid content -no peril admit space/porin channel -no endotoxin |
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Gram - cell wall:
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-very thin
-cell wall had murein lipoprotein -contains 3 layers -high lipid content -had peri plastic and porin channel (resistant to breakdown by antibiotic) -outer cell membrane contains edotoxin (lipid A) lipidopolysaccharide -design allows cell to block diffusion of substances that attack cell walls such as penicillins and lysozyme |
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Not all organisms are susceptible to gram staining and therefore require specific considerations.
-both statements true -both statements false -first true second false -first false second true |
Both statements true
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Gram + cocci are divided into 3 groups based on their ability to
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Hemolysis RBC's
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3 groups if gram + cocci
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- Beta hemolytic streptococcus (completely lose RBC's)
- Alpha hemolytic streptococcus (Partially lose RBC's) - Gamma hemolytic streptococcus (unable to lose RBC's) |
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Beta hemolytic streptococcus hemolytic about is due to the production of
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Hemolysins
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Beta hemolytic streptococcus are arranged in groups
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A-U (known as lance field groups)
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Which beta hemolytic streptococcus group is the most important human pathogens
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A
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Many beta streptococcus stains are
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Anti-phagocytic |
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S. Pyrogens produces _____ that dissolves clots making further invasion possible and ____ (spreading factor)
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-streptokinase
-hyaluronidase |
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Hyaluronidase is necessary for ____ tissues during spread of infections
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Hyaluronidase
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Group A have the ability to adhere to ____ epithelium
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Pharyngeal epitherlium
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Group A streptococci are causative for maladies such as:
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-streptococcal pharyngitis
-Ptolemaic infections -tonsillitis -scarlet fever/ rheumatic fever |
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What two organisms are not susceptible to gram staining in the dental deild
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-Myobacteria |
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Inflammatory disease that may develop after an infection with streptococcus bacteria(such as strep throat or scarlet fever) and can involve the heart,joints, skin, and brain
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Rheumatic fever
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The causative organism for scarlet fever and rheumatic fever is
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Streptococcus
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Viridans group of streptococcus
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-big heterogenous group of streptococcus
-no lance field group -not bile soluble -greenish tint when cultured -alpha hemolytic |
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Myobacterium require a ____ stain
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Acid fast
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Tuberculosis is a disease caused by the bacteria called |
Mycobacterium Tuberculosis
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Tuberculosis is a _____ infection
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Bacterial
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Tuberculosis is acquired via ______ transmission |
Airborne
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Spirochettes (tripomema dentricola) require __________ microscopy
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Dark feild
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Most disease causing microorganisms are
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- gram - rods
- gram + oleo morphia bacteria |
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Gram + cocci |
- non mobile |
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Streptococcus have spherical gram ______ cocci usually arranged in _____ or ______ |
+ chains or pairs |
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All _____ are catalase negative |
streptococcus |
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streptococcus are divided into 3 groups based on their ability to _______ |
hemolyse RBC's |
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Beta-hemolytic streptococcus ____ lyse RBC's |
completely |
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Alpha-hemolytic streptococcus ____ lyse RBC's |
partially |
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Gamma-hemolytic streptoccocus ____lyse RBC's |
unable |
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What are the 3 groups of streptococcus |
-Beta hemolytic -Alpha hemolytic -Gamma hemolytic
*think of BAG a RBC |
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what streptococcus has the hemolytic ability is due to production of hemolysins |
beta bemolytic |
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what streptoccous arranged in group of A-U (known as lancefeild group) |
beta hemolytic |
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Group ____ are among the most important human pathogens |
A |
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Many strains of ______ streptococcus are anti-phagocytic |
beta |
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S. pyrogens produces ______ and ______. |
streptokinase and hyaluronidase |
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dissolves clots making further invasion possible |
streptokinase |
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"spreading factor"; necessary for invading tissue during spread of infections |
hyaluronidase |
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Partially due to their ability to adhere to pharyngeal epithelium group ____ streptcocci are causative for many maladies |
A |
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Group A streptococci are causative for what maladies |
-streptoccal pharyngitis -pyogenic infection -tonsillitis -scarlet fever rheumatic fever |
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inflammatory disease that may develop after an infection with stretococcus bacteria (such as strep throat or scarlet fever) and can involve the hear, joints, skin, and brain |
rheumatic fever |
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causative organism for scarlet fever and rheumatic fever is ______ |
streptococcus |
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Big, heterogeneous group of streptococcus |
viridans group |
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what group of streptococcus has no lancefield group |
viridans group |
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what group of streptococcus is not bile soluble |
viridans group |
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viridis is latin for |
green |
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organisms that produces greenish tint when cultured |
viridis |
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most of the ___ group is alpha hemolytic |
viridans |
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normal inhabitants of nasopharynx and gingival crevices is what group of streptococcus |
viridans |
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viridans group of streptococcus cause what 3 main types of infections |
-dental infections -endocarditis -abscesses |
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____ bind to teeth by producing dextran polysaccharides in the presence of sucrose (also known as glycans) can produce acid and dental caries. |
s. mutans |
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_____ group produces acid and dental caries |
viridans |
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S. mutans metabolizes ____ to ____ which creates an acidi |
sucrose lactic acid |
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Sucrose is also used by S. mutans to produce _______ |
dextrans |
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only sugar that can be utilized to form dextrans |
sucrose |
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allows strep to adhere to the tooth and add to the volume of plaque |
dextrans |
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sugar besides sucrose that can be digested by S. mutans |
glucose fructose lactose |
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when glucose, fructose, and lactose are digested by S. mutans the end product is |
lactic acid |
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the combination of ____ and _____ leads to dental caries |
plaque and acid |
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S. mutans is only involved in ____ not ______ |
caries process perio |
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dental manipulations can release ____ into the bloodstream and cause endocarditis |
viridans |
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viridans in the bloodstream will implant on the _____ surface of the heart (most commonly damaged heart valves) by producing extracellular _____ |
enocardial dextran |
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subgroup of viridans variety known as ______ and ______ are associated with dental, brain and abdominal abscesses |
S. intermedius S. anginosus |
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normal inhabitants of the GI tract |
S. anginosus |
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most common viridans organisms |
*s. mutans *s. salivarius s. mitis s. sobrinus s. sanguis
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pit and fissure caires |
s. mutans |
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smooth surface caires |
s. sobrinus |
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colonizes oral tissue; associated with the tongue |
s. salivarius |
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endocarditis caused by what viridans organism |
s. mitis |
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plaque colonization and endocarditis |
s. sanguis |
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spherical, gram + bacteria which colonize the mouth and URT of humans a few hours after birth |
streptococcus salivarius |
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Most common aerobic organisms implicated in facial cellulitis |
viridians streptococcus |
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can be encapsulated with polysaccharide layer which makes it very resistant and destructive |
streptococcus pneumoniae |
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generally harmless component of normal flora |
staphyloccus |
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has powerful arsenal of enzymatic and exotoxin weapons |
staphylococcus |
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True or false: staphylococcus is penicillin-G resistant streptococcus is not |
True |
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Important characteristics of staphylococcus |
-non mobile -non spore forming -spherical gram + cocci arranged in grapelike clusters -facultative aerobe -typical lesion of staphylococcus is abscess formation |
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typical lesion of a staphylococcus is ____ formation |
abscess |
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significant exotoxins of staphylococcus aureus are _____ and ____ |
coagulase and hyaluronidase |
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what exotoxins of staphylococcus aureus degrades hyaluronic acid |
hyaluronidase |
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what is the gorund substance of subcutaneous tissue |
hyaluronic acid |
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staphylococcus aureus produces a pigmented compounds called |
carotenoids |
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staphylococcus aureus is a major producer of proteins called |
leukocidins |
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leukocidins destroy |
phagocytes |
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destroyed phagocytes make up much of the material of |
pus |
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what is the most resistant disease causing organism |
MRSA (methicillin resistant staphylococcus aureus) |
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What are the 4 examples of gram + rods (staphylococcus aureus) |
-Bacillus -Clostridium -Corynebacterium -Listeria |
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causative organism for anthrax and food poisoning |
bacillus (gram + rod) |
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causative organism for tetanus, botulism, gangrene, food poisoning, and antibiotic-resistant pseudomemnranous colitis |
clostridium |
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causative organism for diptheria |
corynebacterium |
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has endotoxin; causative organism for meningitis and sepesis |
listeria |
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causes lockjaw or tentanus; normally inhabits superficial layers of soil; spores are genereally DUST BORN |
clostridum tentani |
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Gram - Cocci |
neisseria |
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causative organism for meningitis and gonorrhea |
neisseria |
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what are the gram - spiral shaped organisms |
-spirochetes
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what is found in association with NUP/NUG |
grap - spiral shaped organisms (spirochetes) |
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causative organism for syphilis |
spirochetes: -treponemtosies -lymedisease -leptospirosis |
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can derive lactic acid from glucose which creates and acidic environment; considered aciduric |
lactobacillus |
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______ does not produce dextran but produces a different extra polysaccharide called |
lactobacillus; lexan |
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associated with advanced carious lesion deep in enamel and are aciduric |
lactobacillus |
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gram positive pleomorphic rods which form a large proportion of oral microflora |
actinomyces |
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what are the 3 actinomyces |
-A. naeslundii -A. viscosus -A. odontolyticus |
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have been implicated in root caries although their role in dental caries initiation and progression is not well understood |
actinomyces |
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morphology of cocci |
spherical
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morphology of bacilli |
rods |
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morphology of spiral forms |
comma shaped s shaped spiral shaped |
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morphology of pleomorphicl |
acking distinct shape |
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growth phases of bacteria refer to ______ of cells not _____ cells |
populations individual |
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microbes exhibit 4 distinct phases of growth |
lag phase logarithmic stationary phase death phase |
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what growth phase of bacteria is slow at first as bacteria acclimate to their environment |
lag phase |
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_________ activity was not increase during hte lag pahse |
cellular metabolic |
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what growth phase of bacteria: growth occurs exponentially (very rapidly) |
logarithmic (exponential) phase |
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what growth phase of bacteria: microorgansims are most prone to physical and chemical agents |
logarithmic (exponential) phase |
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what growth phase of bacteria: no net increase or decrease in cell numbers as nutrients are used up and waster products build up |
stationary phase |
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what growth phase of bacteria: after the stationary phase ends, microbes die off |
death phase |
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metabolic characteristics of bacteria can be classified based on what 2 factors |
oxygen metabolism carbon and energy sources |
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what is the most commonly used source of energy and carbon fro humans and bacteria is |
glucose |
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molecular oxygen is (highly or not) reactive |
highly |
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molecular oxygen can form what 3 things |
hydrogen peroxide superoxide radicals hydroxyl radicals |
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bacteria posses 3 enzymes to break down oxygen products |
catalase peroxidase superoxide disumtase |
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breaks down hydrogen peroxide |
catalase and peroxidase |
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breaks down superoxide radicals |
superoxide dismutase |
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what are the 4 bacteria categories based on oxygen metabolism |
obligate aerobes facultative anaerobes microaerophilic obligate anaerobes |
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what do glycoproteins make in the oral cavity |
aquired pellicle |
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humans are what type of aerobes |
obligate aerobes |
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what metabolic bacteria category have all the enzymes ( catalase, peroxidase, superoxide simutase) |
obligate aerobes |
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what are 4 examples of obligate aerobes |
neisseria pseudomonas legionella mycobacterium |
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what metabolic bacteria category is aerobic (use oxygen) |
facultative anaerobes |
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what metabolic bacteria category can grown in the absence of oxygen by using fermentation |
facultative anaerobes |
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what are 4 examples of faculatative anaerobes |
staphylocoecus listeria aetinomyces most other gram - rods |
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what metabolic bacteria category are aeortolerant aneorbes |
microaerophilic bacteria |
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what metabolic bacteria category uses fermentaion |
facultative anaerobes microaerophilic bacteria
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what metabolic bacteria category can tolerate low amounts of oxygen because they have superoxide dismutase |
microaerophilic bacteria |
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what are 4 examples of microaerophilic bacteria |
streptococcus spirochetes camphylobacter denitcola |
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what microaerophilic bacteria is implicated in pregnancy gingivitis |
camphylocater |
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what metabolic bacteria category hate oxygen |
obligate anaerobes |
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what are the 2 examples of obligate anerobes |
prevotella porphyromonas gingivalis (Mr. perio) |
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oblique fibers make up the _____ |
PDL |
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most often associated with hormonal (pregnancy) gingivitis, facial cellulitis, and NUP/NUG |
prevotella |
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possesses an armamentarium of cell surface associated and extracellular activities which are studies intensively for their virulence potential |
porphyromonas gingivalis |
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surface fimbrae allow porphyromaonas gingivalis to adhere to _______ and _______ |
epithelial and tooth surfaces |
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porphyromanas gingivalis is a very imortant pathogen for |
periodontal diseases |
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enzyme capable of breaking down collagen in connective tissue and periodontal attachment appartatus |
collagenase |
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porphyromanas gingivalis produces |
collagenase |