Cytoskeleton 1

Front 1

General features and functions of the cytoskeleton

Back 1

Determine Cell shape and polarity
Mediate cell movements and cell locomotion
Control Organelle movement
Machinery for Cell Division

Front 2

Properties of Intermediate Filaments

Back 2

structural element in cells
Extensive network of rope-like fibers
Form Desmosomes and hemidesmosomes
Form mesh of nuclear lamina
Resist mechanical stress on cell
Associate with microtubules.

Front 3

Composition of Intermediate Filament Subunit

Back 3

3 Domains
-N0globular head domain
-40nm centrol rod domain
-C-terminal globular tail

Front 4

Composition of Intermediate filament bundles

Back 4

2 subunits form dimers through coil
2 dimers go anti-parallel to make building block
tetramers make intertwined filaments within polymer = protofilaments

Front 5

5 types of intermediate filaments

Back 5

I Keratin
II Keratin
III Vementin
IV Nurofilaments
V Nuclear filaments

Front 6

Description of Keratin

Back 6

I is acidic, II is basic/neutral
I has small C-terminal domains
II has large C-terminal domains
All keratins are heterodimers
Can be hard or soft

Front 7

Characteristics of epidermolysis bullosa simplex

Back 7

- severe blistering after minor contact
-mutation in keratin genes in basal cell layer
- prevent keratin from resisting mech. stress

Front 8

Types of Vementin

Back 8

Vimentin- (mesoderm)- endothelia, adipocytes, white cells
Desmin- (muscle) - smooth and skeletal sarcomere z-lines
Glial fibrillary acidic Protein (GFAP)- glial cells, astrocytes, Schwann cells
Peripherin- neurons

Can either homopolymerize or hetero, but not with keratin

Front 9

Properties of Nuclear Lamins

Back 9

Type V IF
A, B, C
Assemble into sheets on inner membrane
regulate NEB and reassembly in mitosis

Front 10

List of IF associated Proteins

Back 10

Epinemin- vimentin, Desmin- muscle
Filaggrin- keratin- epidermis
Paranemin- vimentin, desmin- muscle
Plectin- vimentin- mesoderm
Synemin- vimentin, desmin - mesoderm, muscle