Csf Exam I Lecture 1 Cell Bio Ii

Front 1

both constituive and regulated exocytotic vesicles bud rom the _____

Back 1

TGN

Front 2

vesicles bud from the TGN and are transported along ______ or the cytoskeleton to the PM

Back 2

microtubules

Front 3

The ______ ______ _____ is an "always on" system in which vesicles contiang secretory proteins release their content continuously

Back 3

constitutive exocytotic pathway

Front 4

The _____ ______ _____ is an intermittent one in whcih the vesicles containing secretory proteins accumulate in the cytoplasm until the cell receives a specific signal neural or hormonal whereupoocytosis of the vesicles proceeds rapidly

Back 4

regulated exocytotic pathway

Front 5

what does clathrin do

Back 5

even membrane fusion

Front 6

what three things are clathrin coated

Back 6

regulated exocytotic vesicles
receptor mediated endocytotic vesicles
lysosomes

Front 7

Docking and fusion of vesicles from the TGN involve what proteins of the PM

Back 7

SNARES and SNAPS which are used to target synaptic vesicles

Front 8

______ is a process by which vesicles invaginate fromthe palsma membrane carrying materials into the cell

Back 8

endocytosis

Front 9

what are five types of endocytosis

Back 9

RME
potocytosis
phagocytosis
pinocytosis
transcytosis

Front 10

T or F RME is highly specific

Back 10

TRUE. allows cells to select molecules at the cell surface even in low conc

Front 11

give an overview of RME works

Back 11

highly specific
endocytotic vesicle invaginates
clathrin pinched off
vesicle fuses with endosome
luminal pH drops to 5
receptor-ligand becomes unstable
ligand floats away free

Front 12

in RME after ligand floats away ee what four things might happen

Back 12

receptor recycled and ligand degraded
both recycled
both degraded
both transported through cell

Front 13

T or F clathrin coats potocytotic vesicles

Back 13

FALSE caveolin coats them

Front 14

T or F potocytosis (a form of endocytosis) vesicles bind to endosomes

Back 14

FALSE this only happens with RME

Front 15

where does the little vesicle in potocytosis go?

Back 15

stays near cell surface

Front 16

what three types of integral proteins are invovled in potocytosis

Back 16

receptor
proton pump
channel proteins

Front 17

is TGN involved in potocytosis

Back 17

no

Front 18

T or F. rapid acidification of the vesicle contents resulting from activity of the proton pump and the small size of the vesicle causes relase of the ligand from the receptor in potocytosis

Back 18

True

Front 19

______ is the uptake in vesicles of relatively alrge objects like RBCs or bacteria

Back 19

phgocytosis

Front 20

Phagocytosis most commonly occurs in ______

Back 20

macrophages

Front 21

_______ is a process for the indiscriminant uptake of fluid

Back 21

pinocytosis

Front 22

bulk phase endocytosis is another name for

Back 22

pinocytosis

Front 23

where is the most common place to see pinocytosis

Back 23

endothelium of very small blood vessels

Front 24

what are the two subtypes of pinocytosis

Back 24

micropinocytosis
macro (large vesicles 250 - 500nm)-- this is very uncommon

Front 25

what is our example of transcytosis

Back 25

placenta or breast milk of how to get igGs to baby

Front 26

_____ is the loaded vesicle is transported THROUGH the cell from apex to base or the reverse exocytosis occurs, and the ligand is released

Back 26

transcytosis

Front 27

___________ is a pathway which does not use signal peptides, the ER or the GA but rather proteins to be exported interact directly with acidic phospholipids ofthe PM disrupting the membrane and allowing them to escape the cell

Back 27

non classical secretion

Front 28

T or F. some subtances taken up by a lysosome may not be digestible and may remain in the lysosome for the lifetime of the cell

Back 28

true-- these small remnants are called tertiary or residual bodies (lipofuscin granules)

Front 29

where might you find a lot of lipofuscin granules? why?

Back 29

neuns and myocytes
mostly non-dividing cells so tertiary lysosome residual bodies don't go away when cell dies

Front 30

what does a eukaryotic cell use for large scale degradation

Back 30

proteaome and autophagy. Of these, autophagy has capacity to degrade entire organelles

Front 31

______ is accidental death resulting from injury

Back 31

necrosis

Front 32

type I PCD is the same as

Back 32

apoptosis

Front 33

what is a hallmark of apoptosis

Back 33

condensation of chromatin within the nucles followed by fragmentation

Front 34

what is a common method of controlling cell death

Back 34

proteases called caspases which are used in many cell types to digest various parts of the cell

Front 35

interfering with apoptosis in the hematopoetic system such as with BCL-2 mutations leads to _____

Back 35

leukemias

Front 36

type II PCD =

Back 36

autophagic cell death

Front 37

when would you see type II PCD

Back 37

when starving cell reallocates nutrients from unnecessary processes to more essential processes

Front 38

def. endocytosis of large particles or cells

Back 38

phagocytosis

Front 39

def. RME that is controlled by the interaction of a receptor with its ligand. clathrin coated.

Back 39

RME

Front 40

def. RME that uses a GPI linked receptor. vesicles coated with caveolin

Back 40

potocytosis

Front 41

def. a tertiary lysosome and its contents

Back 41

residual or lipofuscin granule

Front 42

def. what bring sSNARES together to enable membrane fusion

Back 42

SNAP

Front 43

___ are proteins that are foudn in both vesicle and plasma membranes

Back 43

SNARES

Front 44

what do centrioles do

Back 44

entrioles (in the mitotic spindle) nucleate microtubules in cell division, Centrioles (basal bodies) nucleate microtubules in cilia, Centrioles nucleate microtubules for cytoarchitecture and organelle transport