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21 Cards in this Set

  • Front
  • Back

Cell Respiration

*Process called this due to mitochondria giving off CO2 and taking in O2




*Reason why we breathe




*Point of cell respiration- to slowly break down glucose & extract the energy from what we eat & to make it into a usable form for the cell, which is ATP




*ATP activates our metabolism to keep us alive





Components of Mitochondria

*cristae


* intermembrane space


*matrix


*double membrane: inner and outer

Mitochondria

*made up of two membranes


* Inner membrane separates the matrix from the inner-membrane space.


*folds in inner membrane are called cristae


*Cristae are embedded with:


*Proteins that pump protons


* ATP synthase enzymes that generate ATP

Matrix

The open area within the middle of the mitochondria- inner area- rich in enzymes for carbohydrate breakdown

Oxidation

Removal of Hydrogen Atoms


*Hydrogens removed from glucose


*Gives waste product carbon dioxide

Reduction

Addition of Hydrogen Atoms


*Oxygen accepts hydrogens


*Become waste product water

3 Major Parts of Cell Respiration

1.Glycolysis



*Anaerobic (no oxygen)




2.The Citric Acid Cycle (Krebs Cycle)


*Aerobic (oxygen needed)




3. Electron Transport System


*Electron transport


*Proton translocation


*Chemiosmotic phosphorylation



Glycolysis

*Occurs in the cytosol of the cell


* Anaerobic


*Breaks glucose down


*Requires energy (2 ATP) to get started


*Results in:


-Net Gain of 2 ATP


-2 pyruvate molecules


-Electrons and protons carried the ETC

Aerobic Vs. Anaerobic

With air vs. Without air

Aerobic

-After glycolysis, the process proceeds inside of the mitochondria.


-There is a complete oxidation of the organic compound.


-The oxidized compounds release the electrons which are used to produce ATP.

Anaerobic

-Fermentation


-this process does not take place inside of the mitochondria.


-Pyruvate is reduced to form either alcohol or lactate:


*alcoholic Fermentation- In yeast


* Lactic Acid Fermentation- In humans

Krebs Cycle (TCA Cycle)

*Occurs in the mitochondria


-Pyruvate must be transferred from cytosol to the mitochondria




*Each pyruvate molecule goes through TCA, meaning for every glucose molecule, there are two turns of the TCA cycle.




*Electrons and protons carried to the ETC

Citric Acid Cycle

Electron Transport System

*Embedded in Cristae of mitochondria


*Series of carriers pass electrons from one to the other


*NADH and FADH2 deliver electrons



Electron Transport System Cont.

*Electron Transport Chain (ETC)



-Transport electrons through cristae membrane




*Proton Translocation




-Sets up proton gradient across membrane




*Chemiosmotic Phosphorylation



-The electrons from the oxidation reaction of TCA Cycle are used to produce ATP


* every time NAD+ is picked up hydrogen atoms (protons and electrons) to become NADH plus H it shuttled those extra hydrogen atoms to the ET system where they are used to Phophorylate (add a phosphate to) ADP, which yield ATP

Phase 2: Proton Translocation

Translocated from the matrix inside the mitochondria to the inner-membrane space




Creates a proton gradient


-High concentration inside of inner-membrane space (reservoir)


-Low concentration outside of cristae (matrix)

Phase 3: Chemiosmotic Phosphorylation

-The moving of protons through the ATP synthase protein



-Creates a turbine effect of energy production




-ADPs are converted to ATPs

Energy Yield from Glucose Metabolism

Max of 38 ATPs or less




Some make 36 ATPs or less




36-38 ATP is about 40% of available energy in a glucose molecule




Rest is lost to heat



Alternative Metabolic Pathways

*Cells use other other energy sources




*Fatty acids have longer carbon chains- yields more ATP




*Intermediates can also be used to make other products




*Extra food made into fate for storage

Fermentation

*Anaerobic breakdown of glucose




*In the absence of oxygen, NADH passes e- off to pyruvate (reducing it to make either lactic acid or alcohol.


-Generates only 2 ATP total




*Animal cells


-Pyruvate reduced to lactate


-Brief burst of energy for muscle cells


-Recovery from oxygen deficit complete when enough oxygen is present to completely break down glucose


-Lactate converted back to pyruvate or glucose

Microorganisms and fermentation

Bacteria use fermentation to produce


*lactate or other organic acids


*Alcohol and carbon dioxide


Yeast- Carbon dioxide makes bread rise, ethanol made in wine and beer