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5 Cards in this Set
- Front
- Back
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Direct DNA probe detection methods are chiefly used in the diagnosis of diseases in what two organ systems?
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The infectious agents of the genitourinary (sexually transmitted diseases) and respiratory systems are the target of the commonly used direct DNA probes.
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What is the dual role of Thermus thermophilus in the molecular detection of hepatitis C virus (HCV)?
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The enzyme derived from this bacteria can perform both reverse transcription and amplification. Usually, two separate enzymes are needed for these two
tasks. |
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Your laboratory has been using reverse-transcriptase polymerase chain reaction (RT-PCR) to monitor human immunodeficiency virus (HIV) RNA levels for the
hospital's large HIV-positive population. A representative for a company using a different technology for HIV RNA quantification presents a slightly more costeffective arrangement for this assay. What reason can you give for staying with your current RT-PCR technology? |
HN RNA measurements varied from patient to patient among three methods used to quantify this virus in a recent study. The authors of the study recommended that the methods should not be used interchangeably. The teaching point is that there may be variation in results between different methods, or different manufacturers' platforms, for any assay. If you change
your method, you must carefully review and compare your results between the old method and the new, and inform clinicians of any significant changes in interpreting the results. |
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Discuss how molecular techniques may be used in the case of a 55-year-old male housepainter with a cough and an acid-fast bacilli (AFB)-positive sputum
specimen. |
A nucleic acid amplification method would allow the more rapid discrimination of Mycobacterium tuberculosis and M. kansasii so that appropriate
therapy could be started promptly, as well as isolation, if indicated. In real practice, however, false positives of M. tuberculosis occur, limiting the utility of this method. |
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What is the utility in performing molecular typing of HCV?
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There are different strains of HCV, and some respond better to interferon-a than others. Typing allows one to predict the response to treatment.
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