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17 Cards in this Set
- Front
- Back
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Recombinant DNA
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DNA that has been formed artificially by combining constituents from different organisms.
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Restriction Enzymes
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An enzyme produced chiefly by certain bacteria, having the property of cleaving DNA molecules at or near a specific sequence of bases.
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Gel Electrophoresis
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A technique for separating protein molecules of varying sizes in a mixture by moving them through a block of gel, as of agarose or polyacrylamide, by means of an electric field, with smaller molecules moving faster and therefore farther than larger ones.
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DNA Fingerprint
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The use of a DNA probe for the identification of an individual, as for the matching of genes from a forensic sample with those of a criminal suspect.
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Biotechnology
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The use of living organisms or other biological systems in the manufacture of drugs or other products or for environmental management, as in waste recycling.
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Nucleic Acid Hybridization
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A technique in which single-stranded nucleic acids (DNA or RNA) are allowed to interact so that complexes called hybrids are formed by molecules with similar, complementary sequences.
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Polymerase Chain Reaction
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A method to analyze a short sequence of DNA (or RNA) even in samples containing only minute quantities of DNA or RNA.
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Restriction Fragment Length Polymorphisms (RFLP's)
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A difference in DNA between people that can be recognized by the use of a restriction enzyme.
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Restriction Fragment
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A fragment of a DNA molecule cleaved by a restriction enzyme.
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Restriction Site
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The specific sites at which a restriction enzyme will cleave DNA.
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Southern Blotting
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A method routinely used in molecular biology for detection of a specific DNA sequence in DNA samples.
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Cloning Vector
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A small piece of DNA into which a foreign DNA fragment can be inserted.
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Sticky Ends
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An end of a DNA double helix at which a few unpaired nucleotides of one strand extend beyond the other.
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Vaccine
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A substance used to stimulate the production of antibodies and provide immunity against one or several diseases.
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Denaturation
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A process in which proteins or nucleic acids lose their tertiary structure and secondary structure.
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DNA Ligase
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A specific type of enzyme, a ligase that repairs single-stranded discontinuities in double stranded DNA molecules.
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Gene Cloning
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The man-operated process of extracting dna from a host cell and implanting it to function in another type of cell.
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