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22 Cards in this Set
- Front
- Back
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Connectome |
The complete set f synaptic connections liking its 302 neurons (For C. Elegans) |
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Loss of function mutation |
The most fundamental genetic mutation To disrupt the function of an individual gene |
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Reverse genetics |
Refers to strategies for disrupting a pre-designed gene |
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Homologous recombination |
A piece of endogenous DNA sequential for the function of a gene is replaced by a piece of in vitro engineered DNA, the ends of which have sequences identical to the endogenous DNA |
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Cre recombinase |
A bacteriophage-derived enzyme that catalyses recombination between two sequence-specific DNA elements called LoxP |
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FLP recombinase |
Mediates recombination between two FRT sites through a mechanism analogous to Cre-mediated between two LoxP sites |
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Tamoxifen |
The estrogen-binding site of CreER is modified so that it binds tamoxifen nit not endogenous estrogen |
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Genome engineering |
Teh General process of altering the genome at a predetermined locus, wether by deleting a piece of endogenous DNA, inserting a piece of foreign DNA or creating creating specific base-pair change |
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Mitotic recombination. |
DNA recombination occurs between two homologous parental chromosomes in a somatic cell such that one of the daughter cells can be homozygous for part of the parental chromosome |
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Genetic Mosaic animal |
An animal that contains cells of more than one genotype |
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Cell autonomous |
Acts within the cell that produces the gene product |
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Non autonomous |
Acts on cells that do not produce the products |
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BAC - bacterial artificial chromosome |
A cloning vector that can accommodate hundreds of kilo bases of DNA |
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Projection neurons |
Send information of the nervous system to another |
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Local neurons |
Aka interneurons are confined to sending information to within a given region |
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Anterograde tracers |
They are taken up primarily by neuronal cell bodies and dendrites and travel down the axon to label their projections sites |
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Retrograde tracers |
Mostly taken up by axon terminals and transported back to the cell bodies |
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The 3 principal electrophysiological methods that have been employed to record neuronal activity: |
Extracellular recording (single units has been the key method for making conceptual advances in neurobiology) Intracellular recording Whole-cell patch recording |
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Single unit recoding |
Extracellular recording aimed at detecting the firing patterns of individual neurons |
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Local field potentials |
Which are local potentials variations measured relative to a distal ground |
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Harlorhodopsin |
A yellow-light activated inward Cl- pump can be expressed in neurons Used as a optogenetic effector for light-induced neuronal silencing |
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Archaerhodopsin |
A light driven outward proton pump from an archway species Used as a optogenetic effector for light-induced neuronal silencing |
