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9 Cards in this Set
- Front
- Back
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What is the primary site of ER vesicle budding
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Smooth ER
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Are the cisternae tubular in the Smooth ER?
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Yes, but the RER is not tubular, but flat
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What is the ER signal sequence?
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short peptide
-30-35 aa's -usually starts at N-terminus, but can be anywhere - hydrophobic stretch bracketed by polar and charged aa residues |
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Adaptor for ER import
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Signal-recognition particle SRP
-co-translational |
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What is the receptor for ER transport?
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The SRP receptor, on cytoplasm side.
-heterodimer, integral protein -GTP hydrolysis releases SRP for recycling and translation begins |
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What is the ER import Translocator?
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SEC61 brings it in in a heterotrimer complex.
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How does a protein get secreted across the ER?
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The N terminal is targetted to ER, then the signal is cleaved and the protein is in the lumen
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What determines if it will be a type 1 or 2 protein that is imported?
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charge around the signal sequence. Remember that the cytosol is + charged.
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What is the default pathway for a protein from ER?
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rough ER - golgi- PM
need a retention signal or a diversion signal if not. |
