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22 Cards in this Set
- Front
- Back
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Under ideal conditions, an experimental group should have how many variables? |
one |
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Generally, a pipetter is not as accurate at |
either end of its range |
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What is true regarding an enzyme catalyzed reaction? |
-->The reaction occurs at a small region on the enzyme called the active site -->The product of the reaction is a substrate that is chemical changed -->The enzyme forms a complex with the substrate to which it binds |
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Which is NOT true regarding an enzyme catalyzed reaction? |
The enzyme raises the activation energy required for the reaction |
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A researcher wants to determine what effect temperature has on the activity of enzyme XMR over a period of 12 hours. Which of the following would be included in the experimental design? |
-->multiple temperatures -->another enzyme as a control -->gathering data at regular time intervals |
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Hereditary hemochromatosis results in |
the body accumulating too much iron |
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What is true regarding PCR? |
-->PCR can be used as a medical diagnostic tool -->PCR produces large amounts of a specific segment of a template from only trace amounts of starting template -->PCR may be used to detect mutations in the genome |
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PCR coupled with restriction enzyme digest is useful to identify individuals via DNA profiling because |
the number and location of restriction sites will vary from person to person |
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DNA fragments are_____and, therefore_______. |
negatively charged, drawn towards the anode during electrophoresis |
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Which of the following classes of enzymes do restriction enzymes belong to? |
endonucleases |
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In immunoflourescence, the primary antibody recognizes |
a carbohydrate or protein epitope |
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The resolution of a confocal microscope is superior to that of a compound microscope because |
only light from a particular focal plan is viewed |
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You examine an unknown Chlamy mutant that can only swim in a circle. What is the best possible explanation for this observation? |
the organisms have only one flagellum |
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How do flagella beat? |
an associated motor protein uses energy from ATP |
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What are forms of cell motility? |
-->chromosomal movement during mitosis -->a sperm cell swimming to an egg -->a vesicle containing HGH being exocytosed |
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Tubulin dimers are added to regenerating flagella at |
the "+" end |
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What would you expect to find 2 hours after you added media containing colchicine (2 mg/ml) to deflagellated Chlamydomonas? |
some flagella regeneration, but the rate of regeneration is slower compared to flagella regeneration in control media (M) |
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Dibucaine was used during protein isolation to |
cause the cells to lose their flagella |
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Why might you use the Bradford reagent? |
The Bradford reagent turns blue when it binds to proteins in your sample, allowing you to measure protein concentration |
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Why did we create a series of dilutions with BSA (bovine serum albumin) and water to quantify the proteins we isolated? |
to establish a standard curve |
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You pick up a recent copy of the journal Cell, and read "At the functional level, we show that endogenous TAZ is required for self-renewal and tumor-initiation properties in breast cancer cells, and that raising TAZ levels promotes these cancer stem cell-traits." Which section are you most likely reading? |
Discussion |
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In a DNA gel, where are the smallest fragments found? |
The bottom of the gel, while the largest stay up near the top |
