Motility and flagellar length were compared in Chlamydomonas reinhardtii before and after deflagellation. The Chlamydomonas were deflagellated by a low pH shock and were observed for flagellar regeneration in 10 minute intervals for 50 minutes. We found the average number of cells that were motile after 50 minutes of flagellar regeneration was 43.80 cells. The difference in motility before deflagellation and after flagellar regeneration was due to flagellar length. Less motility was observed in Chlamydomonas reinhardtii with flagella that were less than 7 microns in length. The cells reach the optimal flagellar length within 30 minutes after deflagellation. The results of this study suggest that motility is at optimal motility with longer flagella
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We found the average before deflagellation to be 55.56 motile cells and at 50 minutes to be 43.80 motile cells. We conducted a t-test comparing motility of Chlamydomonas reinhardtii before deflagellation and at various time intervals (Table 2). We determined the p-value was less than 0.05 at 8.59 x 10^-10 by comparing before deflagellation and t = 0. With a p-value smaller than 0.05, we determined that there was a significant different in motility initially after deflagellation. We determined the p-value was less than 0.05 at 3.98 x 10^-2 by comparing before deflagellation and t = 50. With a p-value smaller than 0.05, we determined that there was a significant different in motility at 50 …show more content…
A pervious study conducting at Yale University, found that full flagellar regeneration occurred with in 90 minutes after deflagellation (Rosnbaum, et al.,1969). Our results presented evidence that our hypothesis was not supported. The average number of cells that were motile before deflagelltion was 55.56 of Chlamydomonas. The average number of cells that were motile 50 minutes after deflagellation was 43.80 motile cells. The difference in motility was significantly different before deflagellation and after 50 minutes of flagellar regeneration. From our results, we concluded that Chlamydomonas reinhardtii did not regain full motility after 50 minutes of flagellar
We found the average before deflagellation to be 55.56 motile cells and at 50 minutes to be 43.80 motile cells. We conducted a t-test comparing motility of Chlamydomonas reinhardtii before deflagellation and at various time intervals (Table 2). We determined the p-value was less than 0.05 at 8.59 x 10^-10 by comparing before deflagellation and t = 0. With a p-value smaller than 0.05, we determined that there was a significant different in motility initially after deflagellation. We determined the p-value was less than 0.05 at 3.98 x 10^-2 by comparing before deflagellation and t = 50. With a p-value smaller than 0.05, we determined that there was a significant different in motility at 50 …show more content…
A pervious study conducting at Yale University, found that full flagellar regeneration occurred with in 90 minutes after deflagellation (Rosnbaum, et al.,1969). Our results presented evidence that our hypothesis was not supported. The average number of cells that were motile before deflagelltion was 55.56 of Chlamydomonas. The average number of cells that were motile 50 minutes after deflagellation was 43.80 motile cells. The difference in motility was significantly different before deflagellation and after 50 minutes of flagellar regeneration. From our results, we concluded that Chlamydomonas reinhardtii did not regain full motility after 50 minutes of flagellar