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14 Cards in this Set
- Front
- Back
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What are the advantages and disadvantages of surface immobilization by absorption? |
It's simple and cheap it can be used for many different processes and the enzyme is still very accessible by the substrate Enzymes can be lost from the matrix easily |
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What are the main carriers for surface immobilization? |
Cellulose carbon nanotubes or silica |
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When do ionic bonds form in surface immobilization by bonding? |
When the carrier is a polysaccharide |
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What are the advantages and disadvantages the surface immobilization by bonding? |
Enzyme is less likely to be lost enzyme is still very accessible and pH and substrate conc have little effect on enzyme activity Active site shape may be changed in the process |
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In entrapment by a matrix what is the matrix usually made up of? |
Polysaccharides or gelatin |
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What what is the advantage and disadvantages of using entrapment by matrixes? |
It is widely applicable to different processes It is expensive, difficult to entrap enzyme and diffusion of substrates and products in and out of the active site can be slow |
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What membranes are used in entrapment through a membrane? |
Semi permeable polymer based membranes |
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What are the advantages and disadvantages of entrapment through a membrane? |
It is widely applicable to different processes and has a relatively small effect on enzyme activity Expensive and diffusion of products and substrates in and out of the active site can be slow |
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What immobilised enzyme is used to make semi-synthetic penicillins which bacteria are less resistant to? |
Penicillin acyclase |
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What immobilised enzyme is used to produce fructose from glucose to be used in commercial sweeteners? |
Glucose isomerase |
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What immobilised enzyme is used to produce lactose free milk for lactose intolerance? |
Lactase |
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What immobilized enzyme is used to produce pure samples of l-amino acids used in pharmaceuticals? |
Aminoacyclase |
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What enzyme is used to break down starch into glucose syrup? |
Glucoamylase |
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What immobilised enzyme is used to hydrate acrylonitrile to acrylamide to be used in plastics? |
Nitrile hydratase |
