Biochemistry - Fatty Acid Biosynthesis

Front 1

What goes into fatty acid biosynthesis?

Back 1

Acetyl CoA and Malonyl CoA
NADPH

Front 2

What comes out of fatty acid biosynthesis?

Back 2

Palamitate (longest fatty acid chain we can make with 16 carbons with carboxyl end and methyl end)

Front 3

4 stages of fatty acid synthesis (NOT elongation cycle)

Back 3

1. transport of Acetyl CoA to cytosol
2. Carboxylation of Acetyl CoA to Malonyl CoA
3. Conversion of Acetyl CoA and Malonyl CoA to acyl carrier protein (ACP) derivatives
4. elongation cycle

Front 4

4 stages of elongation cycle of fatty acid synthesis (last setp)

Back 4

1. condensation of acetyl ACP and malonyl ACP to acetoacyl ACP
2. reduction
3. dehydration
4. reduction

Front 5

Acetyl CoA carboxylase complex requires? Is composed of (3 things)?

Back 5

Requires Mn2+ and 1 ATP
1. Biotin carboxylase (transfers carboxyl group to biotin)
2. Biotin carrier protein (moves carboxylated biotin into position to transfer carboxyl group to acetyl CoA)
3. Carboxyl transferase (Transfers carboxyl group to Acetyl CoA producing Malonyl CoA)

Front 6

First committed step in fatty acid biosynthesis and importance of intermediate it produces?

Back 6

Malonyl CoA production
Inhibits carnitine acyltransferase, which would catalyze beta oxidation.....
REGULATION!

Front 7

What multienzyme complex catalyzes addition of 2 carbon units (from malonyl CoA)?

Back 7

Fatty Acid Synthase

Front 8

Priming step required by fatty acid synthase?

Back 8

transfer of acetyl group from acetyl CoA to acyl carrier protein (ACP) to form acetyl ACP (2 carbons) to mark for fatty acid synthesis.
Acetyl group then transfered to HS-KSase (part of FA synth)

Front 9

enzyme and what used in first reduction? produces?

Back 9

NADPH used
6-ketoacyl-ACP reductase
D-B-hydroxybutyryl-ACP

Front 10

Enzyme used in dehydration? Produces?

Back 10

6-hydroxyacyl-ACP dehydratase
crotonyl ACP produced
TRANS configuration

Front 11

Second reduction uses and produces? Enzyme?

Back 11

B-enoyl ACP reductase
Produces Butyryl ACP (4 carbons)
NADPH used

Front 12

Where elongation can happen?

Back 12

endoplasmic reticulum (unsaturation reactions)
Mitochondrion

Front 13

What is mammal's limit of double bonds on fatty acids (where must stop)?

Back 13

can't make double bonds beyond C9 = must be ingested