Biochemical Differentiation Of Some Medically Important Gram Positive Cocci Lab 14

Front 1

what category media is helpful in determining the biochemical activities of bacteria

Back 1

differential media

Front 2

blood agar is

Back 2

enriched media because it has nutrients added to support the growth of fastidious bacteria.
a differential media because it distinguishes bacteria based on the type of hemolysis

Front 3

what are hemolysins

Back 3

enzymes that lyse erythrocytes providing hemoglobin to metabolize

Front 4

what are the three types of hemolysis and how can they be distinguished

Back 4

alpha ,partial consumption of hemoglobin released when erythrocytes lyse: _greenish brown coloration
beta, total consumption of hemoglobin released when erythrocytes lyse :-completely clear area
gamma, no visible changes in blood agar because no hemolysins release

Front 5

what is bacitracin ?

Back 5

Bacitracin ( like penicillin and amoxicillin) is an antibiotic that inhibit cell wall synthesis by inhibiting the movement of peptidoglycan building blocks across the cell membrane.

Front 6

which beta hemolytic group A is sensitive to bacitracin?

Back 6

Streptococcus pyogenes

Front 7

is a zone of inhibition around the bacitracin a positive or negative test result

Back 7

A zone of inhibition is a positive test result

Front 8

which chemical is in a taxo P disk

Back 8

(optochin) ethyl hydrocupreine hydrochloride

Front 9

which enzyme breaks down h2o2 into h2o + o2

Back 9

catalase

Front 10

how can you tell that catalase is working

Back 10

bubbling due to 02 released

Front 11

what color is phenol red indicator at neutral pH

Back 11

reddish orange

Front 12

what color is phenol red at an alkaline pH

Back 12

red

Front 13

what color is phenyl red at a acidic pH

Back 13

yellow

Front 14

what is another name for glucose

Back 14

dextrose

Front 15

what does coagulase do

Back 15

coagulase converts soluble fibrinogen in the blood plasma into insoluble fiber threads resulting in a fibrin clot

Front 16

how could you explain the negative test result that should be positive according to the chart

Back 16

either the media was not inoculated or the loop with to hot and the microbes dead when transferred

Front 17

how could you explain a positive test result that should be negative according to the chart

Back 17

contamination

Front 18

Staphylococcus (gram? & 5 things)

Back 18

medically important genus of gram-positive bacteria involved in skin absences such as boils post-operation infections scalded skin syndrome toxic skin syndrome in some food poisoning

Front 19

the nickname from Streptococcus pneumoniae

Back 19

pneumococcus

Front 20

the bubbles releasing a positive catalase test are blank gas

Back 20

oxygen

Front 21

yellow color is a blank result for glucose and mannitol test

Back 21

positive

Front 22

a reddish orange color is a positive or negative result for glucose mannitol test

Back 22

negative

Front 23

a Taxo P disk is use for Streptococcus pneumoniae inhibition test called the blank test

Back 23

Optochin

Front 24

cloat formation following the coagulase test as a positive or negative result

Back 24

positive

Front 25

a is it Greek symbol for

Back 25

alpha

Front 26

y is the Greek symbol for

Back 26

gamma

Front 27

bacteria mix with a drop of 3 percent blank to form the catalase test

Back 27

hydrogen peroxide

Front 28

gram positive cocci fastidious organisms so they grow best on

Back 28

blood agar

Front 29

no bubble prodution is a positive or negative catalase test

Back 29

negative

Front 30

coagulase

Back 30

and exoenzyme that reacts with prothrombin in blood plasma to form a complex which converts soluble fibrinogen and insoluble fibrin threads forming a clot

Front 31

a red color result for glucose our mannitol test indicates a blank pH

Back 31

alkaline

Front 32

hemolysis

Back 32

the destruction of red blood cells is accomplished by organisms that secrete toxins called hemolysins

Front 33

differential media is used to test for the presence of a particular blank produced by a bacterium

Back 33

enzyme

Front 34

taxo A disk are saturated with an antibiotic called

Back 34

Bacitracin

Front 35

blood agar is a blank medium consisting of basic nutrient agar to which whole blood has been added

Back 35

enriched

Front 36

I change from reddish orange to blank is a positive result for glucose and mannitol Test indicating acidic pH

Back 36

yellow

Front 37

B is the Greek symbol for

Back 37

beta

Front 38

. 3 milliliters of inoculated bacteria is mixed with. 3 milliliters of rabbit blank in order to perform the test

Back 38

Plasma

Front 39

Gama hemolysis actually refers to

Back 39

no destruction any delay shins of red blood cells and therefore she has no change in the blood agar around isolated bacterial colonies

Front 40

a blank color result for glucose or mannatol test indicates an alkaline pH

Back 40

red

Front 41

in addition to hemolytic activity streptococci may be classified by blank presentations have 20 different serotypes indicating the pathogenic potential

Back 41

lancefield

Front 42

phenol red

Back 42

is the indicator in the glucose and mannitol broths

Front 43

zone of inhibition around taxo a or taxo P disc is A positive or negative result

Back 43

positive

Front 44

streptococcus

Back 44

medically important genius of gram-positive bacteria involve in strep throat scarlet fever rheumatic fever impetigo flesh eating fasciitis some pneumonia meningitis and even tooth decay

Front 45

regulations and exoenzyme that converts soluble fiber an engine in blood plasma in 2m sociable blank threads resulting in a clot

Back 45

fibrin