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42 Cards in this Set
- Front
- Back
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NEISSERIA |
Species: - Neisseria gonorrhoeae/ Fibonacci - Neisseria meningitidis - Pigmented Neisseria
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General Characteristics: |
Non- motile, aerobic, monophonic and fastidious organism; Non- hemolytic |
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N. elongata |
All are Catalase test(+) except: N. Elongata |
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CYTOCHROME OXIDASE TEST |
Screening test for the Genus Neisseria |
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Reagent: |
Tetramethyl-p-phenylenediamine dihydrochloride (+) result: purple coloration |
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Temperature requirement: |
Room temperature prior to inoculation; unable to tolerate cold temperature |
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CARBOHYDRATE / FERMENTATION TEST |
Special Identification under Genus Neisseria can be made thru CARBOHYDRATES / FERMENTATION TEST |
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MEDIA: |
CTA / Cystine Tryptic Agar |
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INDICATOR: |
Phenol red |
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(+) result: |
Yellow color |
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CANDLE JAR |
To supply an increase CO²; inexpensive or economical way of providing Neisseria with an increase CO2 |
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Gaspak Jak |
For anaerobes |
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»1. Neisseria gonorrhoeae |
Pathogenic bacteria
-Kidney, coffee-bean shaped diplococci intracellular within PMN ( neutrophils) |
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Virulence factor: |
PILI - promotes attachment to host cells
-ONLY FERMENTS GLUCOSE |
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Superoxol test (+) |
A rapid test to identify N. gonorrhea
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Reagent: |
30% H²O² (+)= vigorous bubbling |
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Causes:
A. Gonorrhea ("tulo") |
An STD in which patient is producing infectious discharge |
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B. Opthalmia neonatorum |
Conjunctival form of N. gingerbread; its accumulation is either passed or by an infective mother to the child during deliver |
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C. Perihepatitis or Fitz-Hugh-Curtis Syndrome |
Pelvic inflammatory disease involving liver capsule inflammation leading to the creation of adhesions |
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DACRON RAYON FIBERS |
Use to collect specimen swab |
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Media used for cultivating Neisseria gonorrhoeae:
A.) Thayer Martin Media |
Enriched CAP with 3 antibiotics |
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Contains VCN; |
Vancomycin- Prevents GRAM (+) organisms Colistin- Prevents GRAM (-) organisms Nystatin- antifungal
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B.) Modified Thayer Martin Media |
Contains VCN-T |
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Trimetophrim lactate |
To prevent the growth and swarming of proteus and to inhibit Capnocytophaga spp. |
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C.) Martin Lawis Media |
Contains VCAT |
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Anisomycin |
Antifungal; to improve inhibition of Candida albicans |
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D.) New York City Media |
Contains VCAT |
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Amphotericia B |
Antifungal |
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»2.) Neisseria meningitidis |
Normal flora in the nasopharynx region |
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Bean shaped diplococci |
Sensitive to SPS |
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Virulence factor: |
CAPSULE; Neufeld Quellung Test (+); |
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FERMENTS |
MALTOSE and FRUCTOSE |
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Causes: A.) Bacterial Meningitis |
Meningitis in patient between 5-29 years |
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B.) Meningococcemia |
Presence of N. Neningitidis in the blood |
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C.) Waterhouse Friderichsen Syndrome |
Severe from of meningococcemia characterized by the bleeding of the adrenal glands |
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Neisseria lactamica |
Glucose, Maltose, Lactose(+) and ONPG (+) |
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TRANSPORT MEDIA FOR NEISSERIA Transgrow bottles |
Flat bottles coated with MTM and a bottles under increased CO²; rarely used nowadays |
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JEMBEC ( John E. Martin Biological Environment System) |
Used a tablet to generate CO²; Frequently used |
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MORAXELLA Moraxella catarrhalis |
Normal Flora -Morphologically and biologically resembles Neisseria -Encapsulated, with Pili, Non-motile -Catalase(-), on BAP : Gamma-hemolytic -Normal flora of oro and nasopharynx but may cause otitis media |
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ASACCHAROLYTIC |
Does not degrade sugar/ ferment CHO; usually inert |
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BUTYRATE DISK TEST/ Tributyrin test (+) |
Rapid test to detect the enzyme butyrate esterase |
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HOCKEY PUCK |
Colonies remaining intact when pushed across the plate using inoculating loop |
