Biochemical Activities Micro Test

Front 1

Define the function od exoenzymes

Back 1

exoenzymes are often required to break down food materials needed as a source of
energy

Front 2

Define the function od endoenzymes

Back 2

Metabolic processes involve endoenzymes

Front 3

Name the enymes in starch

Back 3

amylase

Front 4

Name the enzymes in lipid

Back 4

lipases

Front 5

Name the enzymes in casein

Back 5

proteases

Front 6

Name the enzyme in gelatin hydrolysis

Back 6

gelatinase

Front 7

name the media used in the test for activities of microorganisms

Back 7

starch agar, lipid agar, milk agar and nutrient gelatin deep tubes.

Front 8

Name the reagents in the test for activities of microorganisms

Back 8

gram's iodine solution

Front 9

What willl the appearance look like in the test for activities of microorganisms

Back 9

starch will have blue black color = positive or a clear zone surrounding the organism = negative. lipid will retain its opacity = negative or loss its opacity = positive Milk will loss its opacity = positive or stay opaque = negative

Front 10

identify and name positive organisms

Back 10

Starch = s. aureus
Milk = B. cereus, P. aeruginosa
Lipid= B. cereus, S. aureus

Front 11

define fermentation

Back 11

A biooxidative process not requiring oxygen in which an organic substrate serves as the final electron acceptor

Front 12

define cellular respiration

Back 12

Aerobic- bioxidations in which molecular oxygen can serve as the final electron acceptor.
Anaerobic- biooxidations in which inorganic ions other than oxygen, such as NO or SO can serve as the final electron acceptors.

Front 13

Name the alternative pathways to Glycolysis and the microorganisms that use them

Back 13

Pentose phosphate pathway (photosynethetic organisms)
entner Doudoroff pathway ( E. faecalis and P. aeruginosa)

Front 14

What is the end product of carbohydrate fermentation

Back 14

metabolism of pyruvate but this is not the reaction for everything

Front 15

define the role of the durham tube

Back 15

its an inverted inner vial for the detection of gas production

Front 16

what is the pathway used by strict anaerobes

Back 16

Anaerobic cellular respiration

Front 17

Name a microorganism that is strict anaerobes

Back 17

Clostridium perfringens

Front 18

Name the media use in and the positive microorganisma and explain the positive reaction that takes place during carbohydrate fermentation

Back 18

The media used is a nutient tube. It changes from red to yellow wit bubbles. The positive organisms are

Front 19

What is a triple sugar iron test used for

Back 19

It is used to differenciate among the different groups or genera of the Enterobacteriaceae, which are all gram negative bacilli capable of fermenting glucose with the production of acid, and distinguish between other gram negative intestinal bacilli.

Front 20

what is the concentration of TSI

Back 20

TSI agar slants contain lactose and sucrose in 1% concentrations and glucose in 0.1% concentration

Front 21

what are the 3 fermentative activities in tsI test

Back 21

glucose fermentation- alkaline slant (red) and acid butt (yellow) with or without gas production (breaks in the butt) lactose and/or sucrose fermentation acid slant yellow and acid butt (yellow) with or without gas production. No carbohydrate fermentation alkaline slant (red) and alkaline butt (red) or no change ( orange- red butt)