Biochem Testiii Lec 27

Front 1

Explain what a GTPase is and it's cycle?

Back 1

Front 2

What are GEF's? What do they do?

Back 2

Guanosine Exchange Factors
completely exchange GDP for GTP

Front 3

What are GAP's, what do they do?

Back 3

GTPase Activating Protein

Front 4

Protein synthesis elongation begins when 70S initiation complex is ______________.

Back 4

Protein synthesis elongation begins when 70S initiation complex is completed.

Front 5

Protein synthesis elongation begins when 70S ___________ ___________ is completed.

Back 5

Protein synthesis elongation begins when 70S initiation complex is completed.

Front 6

Protein synthesis ____________ begins when 70S initiation complex is completed.

Back 6

Protein synthesis elongation begins when 70S initiation complex is completed.

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Front 14

Which protein synthesis EF's are GTPase's?

Back 14

EF-Tu and EF-G

Front 15

What protein synthesis EF is a GEF?

Back 15

EF-Ts

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Front 21

How does the proofreading in the A site of the ribosome work?

Back 21

The slow GTP hydrolysis on EF-Tu while in the site allows for time to proofread.

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Front 26

What do you notice in the A,P, and E sites after the formation of the peptide bond between the A and P sites?

Back 26

That the tRNA's have shifted and the one in the P site is now partially in the E site, and the one in the A site is now partially in the P site.

Front 27

After the peptide bond formation between the __ and __ sites, there is an uncharged tRNA left in the __ site.

Back 27

After the peptide bond formation between the P and A sites, there is an uncharged tRNA left in the P site.

Front 28

After the peptide bond formation between the P and A sites, there is an ____________ tRNA left in the P site.

Back 28

After the peptide bond formation between the P and A sites, there is an uncharged tRNA left in the P site.

Front 29

After the ______________ _____________ formation between the P and A sites, there is an uncharged tRNA left in the P site.

Back 29

After the peptide bond formation between the P and A sites, there is an uncharged tRNA left in the P site.

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Front 38

Three steps of protein synthesis elongation:
1) binding of second aminoacyl tRNA
2) peptide bond formation
3) ?????????????

Back 38

Three steps of protein synthesis elongation:
1) binding of second aminoacyl tRNA
2) peptide bond formation
3) translocation to the next triplet

Front 39

Three steps of protein synthesis elongation:
1) binding of second aminoacyl tRNA
2) ??????????
3) translocation to the next triplet

Back 39

Three steps of protein synthesis elongation:
1) binding of second aminoacyl tRNA
2) peptide bond formation
3) translocation to the next triplet

Front 40

Three steps of protein synthesis elongation:
1) ??????
2) peptide bond formation
3) translocation to the next triplet

Back 40

Three steps of protein synthesis elongation:
1) binding of second aminoacyl tRNA
2) peptide bond formation
3) translocation to the next triplet

Front 41

What are the three steps of protein synthesis elongation:

Back 41

Three steps of protein synthesis elongation:
1) binding of second aminoacyl tRNA
2) peptide bond formation
3) translocation to the next triplet

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Front 52

Which release factors associate with the stop codons?

Back 52

RF1 and RF3

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Front 56

The energy released from _____-GTP blows up the ribosome complex.

Back 56

EFG

Front 57

What is the RRF?

Back 57

ribosomal recycling factor

Front 58

___________________ inhibits protein synthesis by binding in the A site and causing the premature release of the polypeptide.

Back 58

Puromycin inhibits protein synthesis by binding in the A site and causing the premature release of the polypeptide.

Front 59

Puromycin inhibits __________________ by binding in the A site and causing the premature release of the polypeptide.

Back 59

Puromycin inhibits protein synthesis by binding in the A site and causing the premature release of the polypeptide.

Front 60

Puromycin inhibits protein synthesis by binding in the __ site and causing the premature release of the polypeptide.

Back 60

Puromycin inhibits protein synthesis by binding in the A site and causing the premature release of the polypeptide.

Front 61

Puromycin inhibits protein synthesis by binding in the A site and causing the _______________ release of the polypeptide.

Back 61

Puromycin inhibits protein synthesis by binding in the A site and causing the premature release of the polypeptide.

Front 62

Puromycin inhibits protein synthesis by binding in the A site and causing the premature release of the ____________.

Back 62

Puromycin inhibits protein synthesis by binding in the A site and causing the premature release of the polypeptide.