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15 Cards in this Set
- Front
- Back
- 3rd side (hint)
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Magnification |
Microscope lenses bend light to produce a larger image |
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Total magnification (Tm)= |
Magnifying power of objective lens × magnifying power of ocular lens |
Mob × Moc |
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Resolution |
The smallest distance between two objects that allows you to tell the two apart |
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Resolving power (RP) = |
Wavelength / (NAcond + NAobj) |
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NAcond |
Numerical aperture of condenser lens |
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NAobj |
Numerical aperture of objective lens |
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NAobj 4x |
.10 |
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NAobj 10x |
.25 |
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NAobj 40x |
.65 |
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NAobj 100x |
1.25 |
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Stain procedure |
- Crystal violet - grams iodine - ethanol - safranin |
4 |
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Crystal violet |
- basic dye (stains all bacteria) - positive charge |
2 |
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Gram's iodine |
Forms a complex with the Crystal violet and the peptidoglycan of gram positive cell walls |
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Ethanol |
- decolorizer - desolves the thin outer membrane of gram negative cells (removing the Crystal violet) - gram positive cells are not affected |
3 |
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Safranin |
- basic dye - counter stain to turn gram negative cells pink |
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