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28 Cards in this Set
- Front
- Back
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1 ml added to 51 ml gives a dilution of --
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1/52; 1:51
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how to prepare microscope for storage
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- loop cord around eyepiece
- specimen holder mechanism centered on stage - put to lowest objective - lower stage as far down as possible - put dust cover on |
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variable you have most control on
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independent variable
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variable you are trying to measure
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dependent variable
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pipette suitable for measuring volumes
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serological pipette
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pipette suitable for transferring volumes
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pasture pipette
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3 main factors of activity of an enzyme
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- enzyme concentration
- pH - temperature |
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the nature of an enzyme activity is --
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specific
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catalyst that increases the rate of reaction by reducing activation energy
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enzyme
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At high activation energy --
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- more products
- higher absorbance - lower transmitance |
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enzyme activity will reach to its maximum at --
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optimal condition
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a blank solution contains --
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all compounds except for what we are trying to measure
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function of an enzyme is directly related to--
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its shape
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the solution you put the solute in
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solvent
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dissolves in solvent
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solute
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3 characteristics of a given protein that can differ from those of another protein
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- solubility
- size - charge |
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ion exchange chromatography separates molecules by --
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charge
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purpose of "salting out"
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concentrates protein and could be utilized as a purification step
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why do we need to desalt protein before we start the batch ion exchange chromatography step
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to concentrate the protein and so the salt doesn't interfere with the charge of the protein
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dilution factor equation
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final volume of dilution/original sample volume
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the diameter of the circle of view you can see when looking down a microscope
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field of view
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the higher the magnification --
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the smaller the field of view
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as you go from low to high power, the light intensity --
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decreases
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isotonic solution
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the net movement of water molecules is zero
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hypotonic solution
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more water will move inside the cell than moving outside, therefore cell wall will swell up and burst
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hypertonic solution
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the concentration of solute molecules outside the cell are more than inside, therefore water will move out from the cell and cell will shrink
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osmosis
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movement of water molecules across a semi permeable membrane from lower concentration of solutes to high concentration
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diffusion
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movement of solute molecules across a semi permeable membrane from higher concentration of solutes to lower concentration
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