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6 Cards in this Set

  • Front
  • Back

Oxidase Test

to determine if an organism produces the enzyme cytochrome oxidase




Blue/Purple=Positive


Colorless= Negative




*Must read within 30 secs to be accurate*

PDA


Phenylalanine Deaminase




(Starts out yellow)

To determine if an organism can produce PDA




Add 6-8 drops of 10% ferric chloride




Dark Green= Positive


Remains tan=Negative

MacConkey Agar


(starts out red)

Hot pink/ red= Lactose Fermenter


Colorless= Non-Lactose Fermenter

Hektone Enteric Agar


HEA


(starts out green)

Yellow/Orange= Lactose Fermenter


Blue/Green= Non-Lactose Fermenter


Black= H2S producer

Kligler's Iron Agar


KIA


(starts out orange)

To determine if an organism has the ability to ferment lactose and/or glucose, and produce hydrogen sulfide H2S




Yellow= Positive


Lactose on top


Glucose on bottom


Black= H2S



Citrate


(starts out green)

to determine if an organism has the ability to utilize citrate as a sole carbon source




Blue= Positive