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37 Cards in this Set
- Front
- Back
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Generation/Doubling time |
Time required for cell to divide |
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At least 20 mins. |
Time required for cell to divide |
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Lag |
Intense metabolic activity |
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Logarithmic/Exponential growth |
Most active metabolically |
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Autotrophs |
CO2 as sole source of C |
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Lithotroph |
Other name for autoroph |
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Nitrogen |
Can either be in organic or inorganic form |
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Nitrogen |
Used in synthesis of nucleic acids and proteins |
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Potassium, magnesium, calcium |
Cofactors for enzymes |
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Magnesium |
Stabilizes ribosomes, CM and NA |
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Iron Copper Molybdenum Zinc |
Trace elements as cofactors |
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B. Complex Vits. Amino Acids Purines and Pyrimidines |
Growth factors |
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Superoxide dismutase and catalase |
Strict aerobes possess |
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Microaerophils |
Only in low levels of O2 |
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Facultative anaerobes/aerobes |
Ferment carbs to lactic acid and acetic acid |
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Carbon Dioxide |
Initiates growth |
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Moisture-H2O |
Major component of cytoplasm |
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Moisture |
Major constituent of culture media |
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6.5-7.5 |
Clinically relevant pH |
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Acid or alkaline pH |
pH for preserving food |
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Ionic strength and osmotic pressure |
Basis in preserving food |
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Psychrophilic/Cryophilic |
Cold-loving |
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Microscopy |
Preliminary information |
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Cultivation |
Taking bacteria from infection by specimen collection and growing them in artificial environment |
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Culture |
Organisms that grow and multiply in a culture medium |
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Petri dish |
Media sterilized before dispensing |
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Test tubes |
Media sterilized after dispensing |
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121 c |
Temperature for autoclave |
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Inspissation |
Media w/ serum or proteins |
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Quality Control |
check for sterility |
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Refrigeration |
storage to prevent dehydration and deterioration |
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Inoculation |
Implantation of specimen into media |
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Sterile cotton swab, inoculating wire loop/needle |
Materials for inoculation |
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18-24 hrs at 37 c |
Proper temp and ventilation for incubation |
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Pseudomonas aeruginosa |
Grape like smell |
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Streak plate |
Most practical and most useful method of obtaining pure culture in solid medoa |
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Pour plate |
Determine approximate no. of viable organisms in h2o and milk |
