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16 Cards in this Set
- Front
- Back
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Test to distinguish types of gram positive cocci
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catalase +/-
Catalase + clusters -> staphylococcus catalase - chains -> streptococcus |
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test to distinguish staphylococcus species
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coagulase +/-
Coagulase+ -> staphylococcus aureus Coagulase- -> Staph epidermis or staph saprophyticus |
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test to distinguish coagulase - staphylococcus species
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novobiocin sensitive/resistant
Novobiocin sensitive -> S. epidermis Novobiocin resistant -> S. saprophyticus |
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Gram positive rod species
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clostridium (anaerobe)
corynebacterium listeria bacillus |
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test to distinguish streptococcus species
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hemolysis
green (partial) hemolysis = alpha hemolytic -> S. Pneumoniae and Viridans streptococci clear hemolysis = beta -> group A (s. pyogenes) Group B (s. agalactiae) No hemolysis = gamma -> enterococcus (e. faecalis) peptostreptococcus (anaerobe) |
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test to distinguish alpha hemolytic streptococci
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capsule (quellung +/-)
optochin resistance bile solubility capsule, optochin sensitive, bile soluble -> S. Pneumoniae no capsule, optochin resistant, not bile soluble -> viridans streptococci |
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test to distinguish beta hemolytic streptococci
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bacitracin resistance
bacitracin sensitive -> group A--s. pyogenes bacitracin resistant -> Group B--s. agalactiae |
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test to distinguish gamma hemolytic streptococci
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anarobe?
not anaerobe-> e. faecalis anaerobe -> peptostreptococcus |
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Coccoid gram negative bacteria speceis
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haemophilus influenzae (requires factors V and X)
Pasteurella--animal bites Brucella--brucellosis Bordetella pertussis |
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Test to distinguish gram negative cocci
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maltose fermentation
+ -> Nesseria meningitidis - -> Nesseriea gonorrhea |
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species which are gram negative rods, and fast lactose fermenters
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klebsiella
escherichia coli enterobacter |
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species which are gram negative rods, slow lactase fermenters
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citrobacter
serratia others |
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species which are gram negative rods, lactose nonfermenters and oxidase -
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shigella
salmonella proteus |
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species which are gram negative rods, lactose nonfermenters, oxidase +
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pseudomonas
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Media for isolating:
1 H influenzae 2 N gonorrhoea 3 B pertussis 4 C diptheriae 5 M tuberculosis 6 Lactose fermenting enterics (e escherichia, klebsiella, enterobacter) 7 legionella pneumophila 8 fungi |
1 chocolate agar with factors V (NAD) and X (hematin)
2 Thayer-Martin (VCN) media 3 Bordet-gengou (potato) agar 4 Tellurite plate, loffler's medium, blood agar 5 Lowenstein-jensen agar 6 Pink colonies on MacConkey's agar 7 Charcoal year extract agar buffered with increased iron and cysteine 8 Sabouraud's agar |
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bugs isolated by:
1 chocolate agar with factors V (NAD) and X (hematin) 2 Thayer-Martin (VCN) media 3 Bordet-gengou (potato) agar 4 Tellurite plate, loffler's medium, blood agar 5 Lowenstein-jensen agar 6 Pink colonies on MacConkey's agar 7 Charcoal year extract agar buffered with increased iron and cysteine 8 Sabouraud's agar |
Media for isolating:
1 H influenzae 2 N gonorrhoea 3 B pertussis 4 C diptheriae 5 M tuberculosis 6 Lactose fermenting enterics (e escherichia, klebsiella, enterobacter) 7 legionella pneumophila 8 fungi |
