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16 Cards in this Set

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Test to distinguish types of gram positive cocci
catalase +/-

Catalase + clusters -> staphylococcus

catalase - chains -> streptococcus
test to distinguish staphylococcus species
coagulase +/-

Coagulase+ -> staphylococcus aureus

Coagulase- -> Staph epidermis or staph saprophyticus
test to distinguish coagulase - staphylococcus species
novobiocin sensitive/resistant

Novobiocin sensitive -> S. epidermis

Novobiocin resistant -> S. saprophyticus
Gram positive rod species
clostridium (anaerobe)
corynebacterium
listeria
bacillus
test to distinguish streptococcus species
hemolysis
green (partial) hemolysis = alpha hemolytic ->
S. Pneumoniae and Viridans streptococci

clear hemolysis = beta ->
group A (s. pyogenes)
Group B (s. agalactiae)

No hemolysis = gamma ->
enterococcus (e. faecalis)
peptostreptococcus (anaerobe)
test to distinguish alpha hemolytic streptococci
capsule (quellung +/-)
optochin resistance
bile solubility

capsule, optochin sensitive, bile soluble -> S. Pneumoniae

no capsule, optochin resistant, not bile soluble -> viridans streptococci
test to distinguish beta hemolytic streptococci
bacitracin resistance

bacitracin sensitive ->
group A--s. pyogenes

bacitracin resistant ->
Group B--s. agalactiae
test to distinguish gamma hemolytic streptococci
anarobe?

not anaerobe-> e. faecalis

anaerobe -> peptostreptococcus
Coccoid gram negative bacteria speceis
haemophilus influenzae (requires factors V and X)
Pasteurella--animal bites
Brucella--brucellosis
Bordetella pertussis
Test to distinguish gram negative cocci
maltose fermentation

+ -> Nesseria meningitidis

- -> Nesseriea gonorrhea
species which are gram negative rods, and fast lactose fermenters
klebsiella
escherichia coli
enterobacter
species which are gram negative rods, slow lactase fermenters
citrobacter
serratia
others
species which are gram negative rods, lactose nonfermenters and oxidase -
shigella
salmonella
proteus
species which are gram negative rods, lactose nonfermenters, oxidase +
pseudomonas
Media for isolating:
1 H influenzae
2 N gonorrhoea
3 B pertussis
4 C diptheriae
5 M tuberculosis
6 Lactose fermenting enterics (e escherichia, klebsiella, enterobacter)
7 legionella pneumophila
8 fungi
1 chocolate agar with factors V (NAD) and X (hematin)
2 Thayer-Martin (VCN) media
3 Bordet-gengou (potato) agar
4 Tellurite plate, loffler's medium, blood agar
5 Lowenstein-jensen agar
6 Pink colonies on MacConkey's agar
7 Charcoal year extract agar buffered with increased iron and cysteine
8 Sabouraud's agar
bugs isolated by:
1 chocolate agar with factors V (NAD) and X (hematin)
2 Thayer-Martin (VCN) media
3 Bordet-gengou (potato) agar
4 Tellurite plate, loffler's medium, blood agar
5 Lowenstein-jensen agar
6 Pink colonies on MacConkey's agar
7 Charcoal year extract agar buffered with increased iron and cysteine
8 Sabouraud's agar
Media for isolating:
1 H influenzae
2 N gonorrhoea
3 B pertussis
4 C diptheriae
5 M tuberculosis
6 Lactose fermenting enterics (e escherichia, klebsiella, enterobacter)
7 legionella pneumophila
8 fungi