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Define Nutrition

A process that a living organism consumes food and uses it for growth, tissue repair, tissue replacement, and elaboration of products

The "___________" is an integration of chemistry, physics, mathematics, genetics, soil science, plant science, animal science and biochemistry.

nutrition

Define nutrient

Any component of the diet that supports normal growth, reproduction, lactation or maintenance of life

What are the six classes of nutrients?

Water


Proteins and amino acids


Carbohydrates


Lipids


Vitamins


Inorganic compounds

What are the three energy producing nutrients?

Proteins and amino acids


Carbohydrates


Lipids

Nutrients that cannot be synthesized by the body are termed "_____________" or "________________."

essential or indispensable

Nonessential nutrients are required in the diet. TRUE OR FALSE

FALSE; essential nutrients are required in the diet

Any edible material that provides nutrients for humans, what is this defining?

Food

Define feed

Any edible material that provides nutrients for animals

Difference between food and feed

edible material for humans v animals

The three energy producing nutrients give


4 ______/gram of energy.

kilocalorie

Define feedstuff (foodstuff)

Any substance made into or used as feed or food, respectively

A mixture of feedstuffs used to provide nutrients to animals. What is this defining?

Diet

Define ration

A daily allocation of feed or food

Animal's nutrients requirementss are also influenced by the:

age of the animal


productivity (ie maintenance of body issues, work, growth, milk, eggs, pregnancy)


the dietary constituents available


other factors

Give examples of life instances where more nutrients are required

Young animals need more nutrients for development


Lactation

What does animal feed contain?

Simple compounds (eg salt or glucose)


Complex mixtures supplied by some plant and most animal products


Organic compounds


Inorganic compounds

Name organic compounds in animal feed:

Proteins containing amino acids


Non-protein nitrogen- containing compounds


Lipids (generally fat-soluble)


Carbohydrates (generally water-soluble)


Vitamins

Macroelements and micro (trace) elements are organic compounds. TRUE OR FALSE

FALSE; inorganic compounds

Both macro and microelements may be present at __________ concentrations in some plant tissues.

toxic

If you ingest an excessive amount of macro and microelements, you'll be fine. TRUE OR FALSE

FALSE; toxic in excessive amounts

Animals contain higher concentrations of _____ and very low concentrations of _____________ (less than 1%).

fat; carbohydrate

Generally, body composition of an adult mammal is about 60% water, 16% _________, 20% _________, and 4% minerals.

protein; fat

The science of nutrition is developed by:

Farmers and animal feeders


Scientists and technologists


Consulting nutritionists and veterinarians

Why has the science of nutrition grown rapidly?

The availability of variety of models, such as cell cultures and animal models

Today, we know more than _____ nutrients are required in the animal diet.

40

Define Proximate Analysis

A combination of analytical procedures intended for the routine description of feedstuffs

Name the different fractions of proximate analysis:

Water


Crude protein (CP)


Ether extract (EE)


Ash


Crude fiber (CF)


Nitrogen-free extract (NFE)

Feed sample--- moisure, 1-----> moisture free sample (dry matter)--------2, crude protein---------------ether extraction, ether extract--------->3--------Boil in acid, boil in alkali, 4-------> Crude fiber + ash------ 5, Ash separated from crude fiber

1. Dry @ 105 degrees celsius


2. Kjeldahl analysis


3. Fat-free residue


4. Nitrogen free extract


5. Burn in furnace

After analysis of DM, nutrient composition can be either expressed on a "___________" or a normal "___________________."

dry basis; as fed basis

How is DM analyzed?

By placing the test material in an oven to evaporate all of the free water

What are the temperatures used for measuring DM?

Usually 100 to 105 degrees celsius

Feed moisture can also be measured with ____________________.

moisture meters

Name the device that give immediate results by means of a probe inserted into the test material.

Moisture meter

Moisture meters are more precise than drying the test material in an oven. TRUE OR FALSE

FALSE; drying in an oven is more precise

Give an example of other equipment to measure DM besides oven and moisture meter.

Microwave oven

Name the subunits of proteins

Amino acids

Name the fat soluble vitamins

Vitamins A,D, E and K

What is the simplest carb?

Glucose: monosaccharide, body uses it as energy (20%)

Macro vs microelements: the body requires more microelements in the diet. TRUE OR FALSE

FALSE; macroelements

Why is dry matter important?

Tells the nutritional value of the feed

Moisture meter isnt as accurate as an over but quicker. TRUE OR FALSE

TRUE

Is there a way to speed up the process of drying DM in an oven?

Increase temperature of oven

Why do we need to measure ash?

We dont, we measure it to know other materials in feedstuff

What is the formula to measure CP?

N (%) x 6.25 = CP

When measuring crude fiber, if you repeat the process over and over, the results will be consistent. TRUE OR FALSE

FALSE; likely to get different results each time

What are we measuring in the Kjeldahl anaysis?

measuring nitrogen relationship

18 to 20% of Volatile fatty acids are produced in ______________.

butyric acid

Determining the DM in the following materials is challenging:

plants that contain high concentrations of volatile fatty acids


plants that contain higher amounts of essential oils, terpenes and other volatile substances


silages or other fermented products that contain large amounts of volatile fatty acids (acetic, propionic, butyric) and ammonia


some sugars that decompose, and partially insoluble proteins at temperatures above 70 degrees celsius

There are several methods to avoid excessive losses of volatiles during measuring the DM. Name them (4)

Drying in vacuum ovens


Freeze drying


Oven drying at 70 degrees celsius or less


Distillation with toluene

How is the 6.25 determined in the CP calculation?

100/16 (16% of nitrogen)

Pros of the Kjeldahl method?

Accurate and repeatable

Cons of the Kjeldahl method?

Time consuming, use of hazardous chemicals, does not distinguish one form of N from another

Crude protein data are valuable for ____________ that can efficiently utilize almost all forms of N.

ruminants

Ether soluble materials include a variety of organic compounds extracted with ________________ for a period of 4 hours or more.

diethyl ether

Some important materials in EE include:

true fats, fatty acid esters and some of the compound lipids


fat-soluble vitamins or provitamins such as the caretenoids

EE method pros?

A valid approach for materials made up primarily of fats and fatty acid esters

EE method cons?

less meaningful if the extract contains large amount of plant waxes, essential oils, resins, or similar compounds

What is the fraction of a feedstuff that has a high caloric value?

EE

The residue remained after the combustible material is burned off. What is this defining?

Ash

What is the temperature used to measure the ash?

500 to 600 degrees celsius

What do higher ash values indicate?

Possible contamination with soil or dilution with other substances

___________________ includes primarily of plant structural carbohydrates such as cellulose and hemicellulose.

Crude fiber, CF

How is crude fiber determined?

By boiling of EE in dilute acid, boiling in dilute base, filtering, drying, and burning in a furnace

What is the equation to determine NFE?

NFE = sample weight - weights of (water, EE, CP, CF, and ash)

What does NFE contain?

Readily available carbohydrates, such as the sugars and starches


Some hemicellulose and lignin, particularly in feedstuffs such as forages

Name the Methods of Analysis

Proximate analysis


Detergent Extraction Methods

What method of analysis involves the Van Soest Method?

Detergent Extraction Methods

Feed sample ----- 1 -------> Neutral Detergent Fiber (NDF) and Neutral Detergent Solubles


l


Boil NDF in Acid detergent, Acid detergent solubles ---> 2--------3, cellulose dissolved -----> 4




What process is this?

1. Boil in Neutral detergent


2. Acid detergent fiber (ADF)


3. Boil in 72% H2SO4


4. Lignin




Van Soest Method

What does NDF include?

Hemicellulose, cellulose, lignin

Neutral detergent solubles:

Soluble CHO, starch, CP, fat




Largely available to all animals for digestion

What is in ADF?

Cellulose, lignin

Acid detergent solubles:

hemicellulose

How does one determine Neutral Detergent Extraction, NDE?

Samples are boiled for 1 hour in a solution containing primarily sodium laurel sulfate

The NDE includes soluble and insoluble fractions - name the solubles:

Solubles such as lipids, sugars, organic acids, and other water soluble material, pectin, nonprotein, etc

The NDE includes soluble and insoluble fractions - name the insolubles:

insoluble (or neutral detergent fiber, NDF) includes cellulose, hemicellulose, and lignin

To determine ____________________, samples are boiled for 1 hour in a solution containing cetyltrimethylammonium bromide in H2SO4.

Acid Detergent Extraction, ADE

The insoluble components of ADE usually referred to as what?

Acid detergent fiber, ADF

What does ADF include?

Cellulose, lignin, and lignified N (indigestible N), cutin, silica, and some pectins

What is bomb calorimetry used to determine?

The energy values of solids, liquids, or gases

______________________________ is used to determine the mineral contents of feedstuff.

Atomic Absorption Spectrophotometry

What is Gas-Liquid Chromatography (GLC) used for?

Analysis of compounds that exist in gaseous form as well as fatty acids

Name some Automated Analytical Equipment

Blood flow meters and blood cell counters


High-pressure liquid chromatography (HPLC)


Nuclear magnetic resonance (NMR)


Mass spectrometry


Gamma and beta counters


Plate readers


DNA synthesizers


Spectrophotometers


Flow cytometers

Define digestions

Preparation of the digesta for absorption

The passage of small molecules from the lumen of the GI tract into the blood or lymph systems. What is this defining?

Absorption

What does digestion include?

Chewing or mastication of food


GI tract muscular contractions


Mixing digesta with chemicals


Enzyme hydrolysis of nutrients

The ___________ is a tubular structure used for:


- ingestion and digestion of food


- elimination of the wastes

GI tract

The stomach is attached to the _______ which is attached to the cecum which is attached to the __________.

small intestine; colon (large intestine)

The GI tract of mammals includes the:

mouth, esophagus, stomach, and small and large intestine

Name associated digestive organs to the GI tract

Liver (secretes bile into the small intestine)


Pancreas (secretes digestive enzymes into the small intestine)

Describe the GI tract of carnivores

They have a gastric stomach and a relatively short and simple intestine


Large intestine is simple and not sacculated


Hind gut fermenters and have limited capabilty of digesting fibrous breeds

Dogs and cats have little to no ________ capacity and an unsacculated colon.

cecal

Define sacculation

A structure formed by a group of sacs. The formation of a sac or pouch

Why are the GI tracts of omnivores more complicated?

To improve utilization of plant tissues

Pig has simple, but long small ___________.

intestine

What do the cecum and large intestine look like in the pig?

Pig has a moderately large cecum and a sacculated large intestine

Pigs and humans are __________ digesters.

colonic

Describe the cecum and the large intestine of the rat

An enlarged cecum and an unsacculated large intestine

The rats are cecal ________________.

fermenters

Describe the GI tract of Sheep

Complex and large stomach


Small intestine is long, but simple


Cecum is relatively large, and large intestine is rather short

Sheep is both pregastric and ___________ fermenter.

hind gut

Describe the GI tract of a horse

Simple and small stomach, with a relatively small intestine


Large cecum, and a very large sacculated large intestine

Describe the GI tract of a rabbit

Medium sized stomach


Short and simple small intestine


Large sacculated cecum


Medium-sized unsacculated large intestine

What is a common behavior for cecal fermenter animals?

Coprophagy (feces eating)

What is coprophagy?

Feces eating

What does Coprophagy allow for?

Cecal fermenters to consume diets with inadequate amounts of essential nutrients

During coprophagy, feces provides the animal with some vitamins and amino acids produced by ___________________ in the GI tract.

microbial activity

Give example of ruminant animals

Cattle, sheep, deer, antelope, camel

Give examples of nonruminant animals

Hamster, monkey, kangaroo, hippo

Dietary habit for cattle and sheep

Grazing herbivores

Dietary habit for deer, antelope and camel

Selective herbivores, including folivores and frugivores

In omnivorous species, what are the incisor teeth used for?

To bite off pieces of the food

In omnivorous species, what are the molar teeth used for?

Mastication of food

The tongue is used a lot by omnivorous species. TRUE OR FALSE

FALSE; used very little

Nonruminants: the tongue lips and teeth are used for ___________ and ____________ food.

grasping and chewing

In carnivorous species, what are the canine teeth used for?

tearing and rending

In carnivorous species, what are the molar teeth used for?

Only to partial mastication and crushing of bones

In herbivorous species, what are the incisor teeth used for?

Adapted to nipping-off plant material

In herbivorous species, what are the molar teeth used for?

Adapted to grind plant fibers

How does the jaw move in herbiorous species?

Both vertically and laterally to efficiently shred plant materials

Saliva is secreted during __________________.

mastication

What are the three bilateral pairs of glands saliva is secreted from?

1. Parotids


2. Sub-maxiallary


3. Sublingual

Where are the parotid glands?

Below the ears

Where is the sub-maxillary gland?

Base of the tongue

Where is the Sublingual gland?

Underneath the tongue

List the functions of saliva (3)

Aiding in formation of food into a bolus


Aiding in the taste mechanisms


Providing a source of enzymes for preliminary digestive processes

Mucosal tissues lining the interior of the stomach of nonruminants are divided into:

Cardiac


Fundus


Pylorie

Cardiac tissue of the stomach:

The cells produce mainly mucus to protect the lining from gastric secretions

Fundus is in the ____________ region.

Peptic gland region

Fundus tissue of the stomach:

contains gastric pits which open into gastric glands

The fundus gland region consist of two types of cells:

body chief or peptic cells


parietal or oxyntic cells

What do the body chief or peptic cells produce?

Proteolytic enzymes

What do the parietal or oxyntic cells secrete?

Hydrochloric acid

What happens in the pyloric region of nonruminants?

Cells produce mucus

The length of the small intestine is the same for all nonruminants. TRUE OR FALSE

FALSE; varies among species

Most of the absorption in the GI tract of nonruminants occurs in the ______________.

Small intestine

What is the small intestine of nonruminants covered by?

Fingerlike projections called "villi"

What do villi do?

Increase the absorptive surface area of the small intestine

Each villi contains what?

An arteriole


A venule


A lacteal

What does a venule do?

Drains into the portal blood system

What does a lacteal do?

A drainage of the lymphatic system

The first section of small intestine is "_____________".

duodenum

What does the duodenum do?

Produces various digestive secretions

What is released into the duodenum?

Digestive secretions from the pancreas as well as bile

Where does absorption occur?

Mainly in the jejunum and ileum, hardly in the duodenum

The large intestine consists of:

Cecum


Colon


Rectum

The size of the large intestine sections vary considerably among species. TRUE OR FALSE

TRUE

What is the function of the large intestine for nonruminants?

An area for absorption of water and secretion of inorganic elements

Considerable amount of _____________________ occurs in the cecum and colon of non-ruminants.

bacterial fermentation

What bacterial fermentation happens in the cecum and colon of non-ruminants?

Synthesis of some of the water soluble vitamins


Synthesis of some of amino acids


Synthesis of volatile fatty acids

______________, some amino acids and other small molecules may be absorbed from the cecum.

Volatile fatty acids

___________ and ___________ play an important role in digestions, absorption and metabolism.

Liver and pancreas

What does the liver produce in non-ruminants?

Bile that functions in emulsification of lipids and micelle formation for improved absorption of lipids

What reduces food size for the avian species?

The beak and/or claws

What is the purpose of the crop in the avian species?

Storage and fermentation

The crop is the second part of the GI tract of avian species. TRUE OR FALSE

FALSE; its the first part

The ______________ is the site for production of gastric juices for avian species.

proventriculus

What in the avian species has similar functions of teeth in mammalian species?

the gizzard

The small intestine enzymes of avian species are similar to ones in mammals; so is absorption. TRUE OR FALSE

TRUE

What enzyme is absent in mammalian and avian small intestines?

Lactase

The ceca and large intestine of avian species are the major sites of what?

Resorption of water

Some _______________ through bacterial fermentation occurs in the ceca of avian species.

fiber digestion

_____________ have no upper incisor teeth, but a few species have canine teeth.

Ruminants

How do ruminants grasp for food?

They use upper dental pad and lower incisors, lips and tongue

Ruminant are divided into:

roughage eaters


selective eaters


transitional types

Describe the molar teeth of ruminants

They have shaped and spaced molar teeth to chew on only one side of the jaw at one time

The lateral jaw movements in ruminants aid in what?

Shredding tough plant fibers

Ruminants produce copious amounts of __________. (150 L/day in adult cows and 10 L/day in sheep)

Saliva

Saliva functions in ruminants:

A source of N (urea and mucoproteins), P, and Na, which are utilized by rumen microorganisms


A strong buffer that aids in maintaining an appropriate pH in the rumen

In adult ruminants, the stomach may contain _______% of the digesta in the entire GI tract.

65-80%

The ruminants stomach is divided into 2 compartments. TRUE OR FALSE

FALSE; 4 compartments

What are the four compartments of the ruminants' stomach?

Reticulum


Rumen


Omasum


Abomasum

Purpose of reticulum:

transfers the ingested food into the rumen or omasum


facilitates the regurgitation of ingesta for re-mastication

The omasum aids in:

reducing the particle size of digesta


controlling the passage of digesta into the lower tract


absorption of nutrients

The __________ is a large fermentation vat with a high population of microorganisms.

rumen

What is the function of the abomasum similar to?

The glandular stomach of non-ruminants

The GI tract of ruminants is relatively short. TRUE OR FALSE

FALSE; its long

In non-ruminants, the food is mixed with ________________ before microbial digestion in the cecum and large gut.

digestive enzymes

In ruminants, the digestive enzymes _________ the food after initial microbial fermentation in rumen.

attack

The digestive enzyme classes:

Amylolytic


Lipolytic


Prolytic

Amylolytic:

Carbohydrates digestion

Lipolytic:

Lipids digestion

Proteolytic:

Proteins digestion

Where are digestive enzymes found?

Saliva (eg amylase)


Glandular stomach or abomasum or proventriculus


Pancreas (eg saccharidases, trypsingen)


duodenum (eg enterokinase)

What is the proventriculus?

A major site of enzymes (eg pepsin) and hydrochloric acid secretion

Salivary amylase is involved in ________________.

Starch digestion

Where is pepsin active at?

Active at rather low pH (below pH 3.5)

Where are pepsin and rennin found?

Digestive enzymes in stomach

What does rennin do?

Important enzyme that coagulates milk into a clot in young suckling mammals

What does the small intestine produce enzymes for?

Digestion of oligosaccharides or disaccharides

What are the digestive enzymes found in the small intestines?

Maltase


Isomaltase


Lactase


Sucrase

Name some complex carbs that are not digested by digestive enzymes. How could they be digested?

xylan, cellulose; microbial enzymatic activity

How are the inactive proteolytic enzymes (proenzymes) converted to active enzymes?

By enzyme produced in intestinal mucosa

Give examples of inactive proenzymes converted to active enzymes

Trypsinogen is activated by enterokinase and trypsin




Chymotrypsinogen and procarboxypeptidase are activated by trypsin

Pancreas releases its secretions directly in the ____________ via a pancreatic duct.

duodenum

What are the saccharidases important for that are produced by the pancreas?

In hydrolyzing starch, glycogen, or dextrins to maltose

After emulsifying the fats by action of bile salts, they are digested by what?

Pancreatic and intestinal lipases

What are the two basic functions of water?

Component of body metabolism


Body temperature control

Water is required for all biochemical reactions in the body as:

Solvent


Transport medium


Diluent


Hydrolytic and oxidative agent

What is produced from the oxidation of organic components in the cells?

Metabolic water

The metabolic water produced per gram of ________ is much higher than for protein or carbs.

fat

1 gram fat -------?----------> 1.07 g H2O per g

oxidized

'Fats (lipids) are more "_______________."

hydrogen dense (more H per gram)

List the properties of water that allow it to control body temperature (5)

high specific heat


high thermal conductivity


high latent heat of vaporization


ready transfer of heat


loss of large amounts of heat on vaporization

In ruminants, water is absorbed from the most sections of the _________________.

GI tract

Where is there a net absorption of water in ruminants?

From the rumen and omasum

In ruminants, there is a net inflow of water in the ___________, __________, ____________, and large gut.

Duodenum


ileum


Jejunum

What does osmotic pressure do, as in, what is its function?

Water absorption

Other factors that can affect water absorption include: (List at least 3 of them)

- Polysaccharides such as pectin reduce water absorption


- Indigestible fibers reduce water absorption


- Diarrhea promoting factors reduce water absorption

Name diarrhea promoting factors that reduce absorption: (4)

inappropriate diets


microbial toxins


Altered osmotic relationships


Physiological reactions

Body water __________ with age.

goes down

What is water content of the body influenced by?

The age of the animal


Fat contents of the tissues(inverse relationship)

The water content of the body one the basis of the ________________ is relatively constant among species (70-75%).

fat-free mass

What comprises the greatest % of water in the body tissues? How much exactly?

Intracellular fluids; 40% of body water

What does interstitial mean?

Outside the cell

Extracellular water includes:

Interstitial fluids


Blood plasma (6%)


Lymph, synovial and cerebrospinal fluids

What is the second largest water compartment?

Extracellular water (1/3)

The body fat contains the most of intracellular water. TRUE OR FALSE

FALSE; the muscle

Body water content is a function of:

physiological stage


sex


diseases

Other sources of body water include:

contents of the GI tract


contents of urinary tract

What is the extracellular fluid volume regulated by?

Osmotic pressure

The extracellular fluid volume is regulated by osmotic pressure:

sodium, the major cation in blood


thirst and antidiuretic hormone (ADH)


Adrenal gland and kidney controls

The rate at which body water is excreted and replaced in the tissues. What is this defining?

"Water turnover"

Nonruminants have higher water turnover than ruminants. TRUE OR FALSE

TRUE

Water turnover is influenced by ___________________.

climate factors

Name water sources in animal's tissues: (5)

- drinking water


- feed water


- metabolic water


- water produced from metabolic reactions


- preformed water associated with body tissues

Name ways water is lost from the animal body: (4)

- urine


- feces


- insensible water


- sweat

Where does sweat come from?

the sweat glands in the skin

Where does insensible water come from?

Vaporization from the lungs and dissipation though the skin

What is water drinking induced by?

Dehydration of body tissues and reduction of salivary flow


Oral sensations through receptors in the mouth

Water consumption is a function of:

energy consumption


body surface area in nonstressing situations


heat stress


temperature and seasonal differences

Total water intake can be influenced by the _________________ of the feed consumed.

water content

What increases by high levels of dietary protein, fat and salt (NaCl)?

Water intake

Give an example of a feed that would increase water intake?

Silages

There is a high correlation between _______________ and water intake.

dry matter intake

What environmental factors increase water consumption?

Temperature


Humidity


Accessibility to water

How much water per kg of dry feed is required?

2 - 5 kg water

Animals with high adaptability to __________ environments drink more water.

wet

Why do sheep drink less water than cattle?

They conserve more water therefore they require less

Birds require more water than mammalian species. TRUE OR FALSE

FALSE; mammalian require more water than birds

Adult animals usually require less water per unit of body weight than young animals. TRUE OR FALSE

TRUE

Physiological conditions such as ____________ and pregnancy will increase water consumption.

lactation

Less active animals need more water than active and nervous animals. TRUE OR FALSE

FALSE; active and nervous animals require more water

Name 3 common effects of water restriction: (6 listed)

- reduced food intake and productivity


- reduced urine and fecal water excretion


- body dehydration and weight loss


- increased respiration rate


- increased blood concentration


- nausea and problems with muscular movements

Give examples of monosaccharides

Glucose, fructose, galactose

Define monosaccharide

1 molecule of sugar

Define disaccharide

2 molecules of sugar

Give examples of disaccharide

sucrose and lactose

Define oligosaccharide

3 - 10 sugar units

Give examples of oligosaccharide

fructo-oligosaccharides

Define polysaccharide

>10 sugar units

Give examples of polysaccharides

Cellulose and starch

Give the structure of simple carbs

CnH2nOn

Give the structure of most complex carbs

CnH2n-2On-1

_____________________ are polyhydroxyl aldehyde and ketones.

Carbohydrates

What is the most important function of dietary carbohydrates?

Provide energy

What is triose and what type of sugar is it?

3 carbon monosaccharide

Give an example of a triose

Glyceraldehyde

What is tetrose and what type of sugar is it?

4 carbon monosaccharide

Give an example of a tetrose

D-Erythrulose

What is pentose and what type of sugar is it?

5 carbon monosaccharide

Give examples of pentose sugars (3)

Ribose


Deoxyribose


Xylose

Where can ribose be found?

Can be found in any living cell


Component of ATP and RNA

Where can deoxyribose be found?

Component of DNA

Where is Xylose found?

Found in woody parts of plants


Hay, straw, hulls, corn cobs


Components of hemicellulose

What is hexose and what type of sugar is it?

6 carbon monosaccharide

Define alpha-D-glucose

Primary energy source


Units of polymers such as starch and cellulose

Give an examples of hexose

alpha-D-glucose


beta-D-galactose


beta-D-fructose

Where is beta-D-fructose found?

component of table sugar (sucrose)

Where is beta-D-galactose found?

Component of milk sugar (lactose)

Glucose + fructose =

Sucrose

What is sucrose?

Disaccharide


Quick source of energy (table sugar)


Found in sugar beets, sugar cane and molasses

Glucose + galactose =

Lactose

What is lactose?

Disaccharide


Commonly found in milk sugar

Glucose + glucose =

Maltose


Cellobiose

What is maltose?

Disaccharide


Derived from starch digestion

What is the difference between maltose structure and cellobiose structure?

The linkage is different, 1,4 carbons v 1,6 carbons

What type of sugar is starch?

Polysaccharide

25-30% of starch in this form


Straight chain of alpha-D-glucose


Soluble in hot H2O


What is this describing?

Amylose

Name two types of starch? If they are starch, what type of sugar is it?

Amylose and Amylopectin; Polysaccharide

70-75% of starch in this form


Straight chain of alpha-D-glucose


Insoluble in hot H2O


What is this describing?

Amylopectin

Starch has two configurations of glucose polymers:

Amylose (linear structure)


Amylopectin

Both amylose and amylopectin are glucose polymers linked via a ___________________ linkage.

glucose-(1,4)-alpha-glucoside

Name some polysaccharides

Starch


Glycogen


Cellulose


Lignin

Glycogen has glucose-____-alpha and ______-alpha linkages

1,4 and 1,6

Where is glycogen found?

Carbohydrate storage in liver and muscle of animals

Where is cellulose found?

Primary carbohydrate found in plant kingdom


About 50% of dry matter of plant


Most abundant organic compound in nature

Plant cell walls are made up of what?

Cellulose


Hemicellulose


Lignin

What increases with plant age?

Lignin

What is the major component in the plant cell wall?

Cellulose

The amount of lignin in feedstuff:

vegetables and cereals

Cellulose is glucose polymers linked via a ______________________________ linkage.

glucose-(1-4)-beta-glucoside

Where is lignin found?

Primary structural component of mature trees

Only ____________________ are able to be absorbed from the GI tract.

monosaccharides

What are poly,tri and disaccharides hydrolyzed to?

Monosaccharides

How are poly,tri and disaccharides hydrolyzed to monosaccharides?

Digestive enzymes of the host


Microflora inhabiting the GI tract

The _____________________________ cannot hydrolyze glucose-4-beta glucoside linkage.

carbohydrate-splitting enzymes

How is the glucose-4-beta-glucoside linkage of cellulose hydrolyzed?

Gut microflora produce cellulase

Anaerobic fermentation of cellulose results in the production of ___________________________.

Volatile fatty acids (VFAs)

VFAs include what?

Acetic, propionic, and butyric acids, which supply energy

Amylose and amylopectin are hydrolyzed to _____________.

glucose

Where does monosaccharides absorption mainly occur?

Duodenum and jejunum

Glucose and galactose are absorbed how?

By active transport (energy-dependent) mechanism

Monosaccharides are absorbed selectively from the GI tract of the rat:

galactose and glucose>mannose>arabinose

Glucose is absorbed by which two transporters?

Sodium-glucose cotransporters

Facilitative glucose transporters

Where are sodium-glucose cotransporters found?

Intestine and kidney

What are sodium-glucose cotransporters?

transportation against its concentration gradient

What are facilitative glucose transporters?

Transportation down a concentration gradient

Name some facilitative glucose transporters

6 Na-dependent glucose transporters


13 facilitative sugar transporters

What controls glucose transporter activity in adipose and muscle?

Insulin and glucose

What is fructose converted to?

Glucose and also lactic acid

Some monosaccharides are converted to glucose within the ______________________ cell.

intestinal muscosal

Sugars share a common pathway for absorption, so they have a ___________________ between each other.

competitive inhibition

Xylose absorption happens through active transport. TRUE OR FALSE

FALSE; not through active transport

Xylose does not show competitive inhibition with _____________________.

galactose transport

How is glucose absorption increased?

By chronically restricted food intake

How is absorption of glucose reduced?

By short-term fasting (24-48 hour)

Brush border enzymes




Sucrose -----------?----------> Glucose + Fructose

Sucrase

Brush border enzymes




Maltose --------------?-----------? Glucose + glucose

Maltase

Isomaltose ----------?-----------? Glucose + glucose

Isomaltase

Feeding your mammals with large amounts of sucrose results in severe _________________.

diarrhea

Young mammals and ruminants produce no or negligible amounts of _____________.

sucrase

Non ruminants have lower amounts of starch-splitting enzymes than ruminants. TRUE OR FALSE

FALSE; ruminants have lower amounts of starch-splitting enzymes than nonruminants

What happens when you feed adult pigs with lactose? Why?

Diarrhea because of a deficiency of lactase

What do young pigs fed with xylose show signs of?

Depressed appetite and growth and eye cataracts

In the absence of diseases the absorption of soluble carbohydrates often exceeds ______%.

90%

Monosaccharides may be converted to glucose and glycogen in the ____________.

liver

What is the process called where monosaccharides are converted to glucose and glycogen?

Glycogenesis

_____________ is the storage form of glucose in liver and muscle tissues.

Glycogen

___________ is a starchlike molecule and can be hydrolyzed back to glucose (glycogenesis).

Glycogen

What does glycogenesis prevent?

Marked elevation in blood sugar

What does glycogenolysis prevent during fasting?

Low blood glucose

What is hyperglycemia?

Elevated blood sugar

What is hypoglycemia?

Low blood glucose

Glycogenesis and glycogenolysis are under control of ____________ and ____________.

insulin and glucagon

What do insulin and glucagon play key roles in?

Maintaining the blood glucose concentration within normal level

What does glycogenesis require?

Two molecules of adenosine triphosphate (ATP) for every molecule of glucose

Glycogen storage in liver and muscle is limited. TRUE OR FALSE

TRUE

When ingestion of carbohydrate exceeds the capacity for glycogen storage, glucose is converted to _______.

fat

Describe the glycolysis process

Glucose (6 C), 2 NAD is transformed into 2 NADH which will go to the mitochondria, 2 ADP transforms into 2 ATP, this process forms 2 pyruvate (3 C) which moves on to the mitochondria, Kreb's Cycle and respiratory transport chain

Describe the Kreb's cycle

Pyruvate turns NAD into NADH and releases CO2, then becomes Acetyl CoA ----> Citrate ----> Oxaloacetate

Each glucose = _____ "turns" of Kreb's Cycle

2

Do more research on the products of the Krebs cycle

...

Define Gluconeogenesis

Formation of glucose from noncarbohydrate metabolites (lipids and amino acids) by liver and, to a lesser extent, kidneys

All of the nonessential amino acids and some of the essential ones are _______________.

Glucogenic

Some of amino acids are both glucogenic and __________.

ketogenic

What are the important lipids in nutrition of humans and animals classified into?

Simple lipids


Complex lipids


Derived lipids

Define simple lipids

Esters of fatty acids (FA) with glycerol or alcohols

Give two examples of simple lipids

oils and waxes

Waxes are esters of FA with ___________.

glycerol

Oils are esters of FA with ___________.

alcohols

Define complex lipids

Esters of FA with an alcohol that contain non-lipid substances

Give examples of complex lipids

Phospholipids


Glycolipids


Lipoproteins

What do phospholipids contain?

Phosphoric acid

What do glycolipids contain?

carbohydrates

What do lipoproteins contain?

Proteins

Lipoproteins can account for greater than 60% of lipids in forage plants (eg galactolipid). TRUE OR FALSE

FALSE; Glycolipids can account for 60% of lipids in forage plants

What are the different parts of glycolipids?

2 fatty acid chains, glycerol and a sugar

The fatty acids in glycolipids are typically ________________.

polyunsaturated

What components make up a phospholipid?

2 fatty acid chains, a glycerol, a phosphate and an alcohol

Phospholipids are an important part of _________________.

cell membranes

Define derived lipids

Derived from simple or complex lipids by hydrolysis

Give examples of derived lipids

Fatty acids (FAs)


Glycerol


Other alcohols

List the most important lipids in animal nutrition:

FA


Glycerol


Triacylglycerols (TG)


Phospholipids


Glycolipids
Lipoproteins


Sterols

What do fatty acids consist of?

A chain of carbon (2-24 or more) and a carboxyl group on the end of chain

What is the general structure of FA?

RCOOH

What is R in a molecular formula?

A carbon chain with variable length

What are the two classifications of fatty acids?

Saturated and Unsaturated FA

Each carbon atom (except the carboxyl group) is attached to two hydrogen (H) atoms. What is this defining?

Saturated FA

Define Unsaturated FA

One or more pairs of Carbon atoms are attached by a double bond, and H has been removed

Name two types of unsaturated FA

cis isomer


trans isomer

What two types of chains are there for FAs?

Straight and branched

____________________ is the common form in animal tissues with an ______ number of carbons.

Straight chain; even

Where are branched FA chains found and with what number of carbons?

in microorganisms and in tissues of ruminants; odd number of carbons

What type of FA chain can be found in fish oil?

Long-chain unsaturated FA

What does the FA chain found in fish oil consist of?

linolenic acid


eicosapentaenic acid


docosahexaenoic acid

The ______________________ depends on the position of the double bond in the carbon chain.

biological activity

Triglycerides are _________ of glycerol and FA.

esters

For triglycerides, FA may be the same or different in all three positions. TRUE OR FALSE

TRUE

The physical and chemical properties of TG are determined by what?

Chain length of FA


Degree of unsaturation of FA

What is the structure of a triglyceride?

A glycerol, 3 esters, and 3 fatty acid chains

How many carbons does a TG with saturated FA need to have in order to be solid at room temperature?

10 or more carbons

How many carbons will usually create a liquid for TG with saturated FA?

fewer than 10 carbons

What can influence the absorption and utilization of TG?

The position of the FA on the glycerol

What constants are used to determine the chemical properties of TG?

The saponification number


The Reichert-Meissl (RM) number


The iodine number

The saponification number has an inverse relationship with _________________________.

molecular weight of fats

What is used as an indicator of the chain length of the FA in the TG?

The saponification

What is the saponification number?

mg of KOH needed for saponification (hydrolysis) if 1 g of fat

What is consumed in saponification?

KOH

ml of tenth-normal KOH needed to neutralize the volatile water-soluble FA released by hydrolysis of 5 g of fat. What is this defining?

RM number

What is the RM number used as an indicator of?

The amounts of volatile FA in TG

Beef tallow has an RM number of _____, but butter has an RM number of __________.

zero; 17-35

What is an indicator of the degree of hydrogenation (saturation) of the FA in the fat?

Iodine number

Tristearin has an iodine number of zero, but linseed oil has an iodine number of __________.

175-202

Define Iodine number

The number of grams of iodine that will be consumed in a reaction with 100 g of fat or oil

The hydrolysis of phospholipids such as lecithin produces:

FA


phosphoric acid


Usually a glycerol


A nitrogenous base

Sphingomyelins do not contain glycerol, but contain what?

FA, choline, phosphoric acid, nitrogenous base, and sphingosine

________________ have higher unsaturated FA than TG in animals.

Phospholipids

What do the emulsifying properties of phospholipids allow them to function as?

Lipid transporters

Phospholipids in animals are more widely dispersed in body fluids. TRUE OR FALSE

TRUE

What is the most abundant sterol in animal tissue?

Cholesterol

Name other important sterols in animals besides cholesterol

ergosterol


7-dehydro-cholesterol


bile acids


androgens


estrogens and progesterones

Where are bile acids secreted from? Where are they stored?

Liver; gallbladder

What does ergosterol yield?

Vitamin D2 after irradiation

What does 7-dehydro-cholesterol yield?

Vitamin D3 after irradiation

What are androgens?

Male sex hormones

What are estrogen and progesterone?

Female sex hormones

Ruminants completely depend on ______________________ to meet their glucose needs.

gluconeogenesis

Why do nonruminants not rely on gluconeogenesis?

They absorb glucose directly

_________ and ____________ are other precursors for glucose synthesis by gluconeogenesis.

Lactate and Pyruvate

Glucose catabolism efficiency of ATP capture = ~______%

34%

Where does the other 66% of metabolic outcome of energy go?

HEAT ENERGY