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24 Cards in this Set

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conform to the standard set by the Reagent Chemical Committee of the ACS analytical work

Reagent Grade

 Extraordinary purity is required Carefully analyzed and the assay is printed on the container label.

Primary-Standard Grade

Chemicals for specific application  Includes solvents for spectrophotometry and high performance liquid chromatography.

Special Purpose Reagent Chemicals

Rules for handling Reagents and Solution

 Select the best grade of chemical available for analytical work.  Replace the top of every container immediately after removal of the reagent.  Hold the stoppers of reagent bottles between your fingers, never set a stopper on a desktop.


 Never return any excess reagent to a bottle.  Never insert spatulas, spoons, or knives into a bottle that contains a solid chemical.  Keep the reagent shelf and the laboratory balance clean and neat.  Observe local regulations concerning the disposal of surplus reagents and solutions.

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Containing vessels must be:

a. marked for positive identification of contents. b. cleaned before being used. c. Washed with deionized water

sudden, often violent boiling that tends to spatter solution out of its container. It causes partial loss of the solution.

Bumping

max cap ranging between 160 and 200g

Maceobalances

max load of 10 to 30g

Semimicroanalytical Balance

capacity of 1-3 g

Microanalytical Balance

The weight of the material is the difference between the two weights of the container before and after the material was transferred from the container

Weighing by Difference

 Looks like an analytical balance  Less precise  Has a tare button like the analytical balance

Top Loading Balance

Materials for weighing

•Weighing Boats


•Weighing Dish


•Aluminum Foils or Weighing Paper

 Plastic for liquid or solid samples  Anti static Facilitate controlled pour off

Weighing Boats

 aluminum or plastic  For liquids or solids  Ideal for controlled pour off, anti –statuc

Weighing Dish

 Moisture resistant and non-absorbent  Must be used if samples must be stored for considerable periods of time

Aluminum Foil or Weighing Paper

How to use the Analytical Balance

Turn the balance on by pressing the keypanel.


Place the weighing paper on the balancepan.


Close the sliding doors


Press the TARE button.


Carefully add the substance to be weighedup to the desired mass.


Before recording mass, close the glass doorsand wait until the stability detector is stable.Record mass.

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Cautions in Using the Analytical Balance

 Center the load on the pan.  Protect balance from corrosion.  Observe proper precautions for the weighing of liquids.  Consult the instructor if the balance needs adjustment.


 Keep the balance and its case scrupulously clean.  Always allow an object that has been heated to return to room temperature before weighing it.  Use tongs or finger pas to prevent the uptake of moisture by dried objects.


CPOC KAU

Avoid putting droppers directly on table tops or surfaces of working area to avoid:

•Contamination


•Skin Iritation


•Damage to property and equipment

1ml is approximately how many drops in a non graduated dropper

20

 Solid to semi-solid particles formed at the bottom of the test tube after combining two reactive solutions

Precipitates

To separate precipitate from supernatant the mixture undergoes

Centrifugation

speeds up separation of precipitate from supernatant using the action of gravity

Centrifuge

Separation of supernatant and precipitate after centrifugation can be done by:

•Capillary Pipet


•Decantation

Ensures complete homogeneity after reagent addition

Vortex Mixer