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3 Cards in this Set
- Front
- Back
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MSA (mannitol salt agar) |
75 g per L & 7.5 g/ 100 ml Selects for staphylococcus Gram + Differential based on mannitol fermintation Mann (-) NO is red Detects staphylococcus epidermidis which has no acid, is alkaline and turns red Mann (+) YES is yellow Detects staphylococcus aureus Acidic and turns agar yellow |
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EMB (Eosin methylene blue agar |
Inhibitors: Eosin, methylene blue, bile salts. Selects for gram (-) Enterics = Enterobacteriaceae E-Coli = used as indicator of fecal contamination of food or h2o Fecal pathogens include: Viruses: polio virus, hep. A, noro virus Bacteria: Vibrio chloerae, shigella dysenteriae, salmonella & campylobactor Protozoa: Entamoeba histolytic, giardia Helminths: Ascaris & taenia solium Emb = differential based on lactose fermintation If it had fermintation that means it makes beta galactidase if it has low acid that means it has dark colonies (enterobacter) if it has high acid that would appear a dark shiny green and would be E-Coli If it doesn't do lactose fermintation it would have no beta galactidase examples would be salmonella, shigella & yersinia pestis
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Blood agar (enrichment) |
Differential based on hemolysis ALPHA HEMOLYSIS " partial/ incomplete hemolysis" Example streptococcus mutans & streptococcus pneumoniae B-BETA HEMOLYSIS Complete hemolysis/ destruction Examples: staphylococcus aureus, streptococcus pyogenes, streptococcus agalactiae Cell wall types: Group A streptococcus (GAS) contains streptococcus pyogenes Group B streptococcus (GBS) contains streptococcus agalactiae which is neonatal sepsis GAMMA HEMOLYSIS Is no hemolysis |
