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MSA (mannitol salt agar)
75 g per L & 7.5 g/ 100 ml
Selects for staphylococcus Gram +
Differential based on mannitol fermintation
Mann (-) NO is red
Detects staphylococcus epidermidis which has no acid, is alkaline and turns red
Mann (+) YES is yellow
Detects staphylococcus aureus
Acidic and turns agar yellow
EMB (Eosin methylene blue agar
Inhibitors: Eosin, methylene blue, bile salts.
Selects for gram (-)
Enterics = Enterobacteriaceae
E-Coli = used as indicator of fecal contamination of food or h2o
Fecal pathogens include:
Viruses:
polio virus, hep. A, noro virus
Bacteria:
Vibrio chloerae, shigella dysenteriae, salmonella & campylobactor
Protozoa:
Entamoeba histolytic, giardia
Helminths:
Ascaris & taenia solium
Emb = differential based on lactose fermintation
If it had fermintation that means it makes beta galactidase if it has low acid that means it has dark colonies (enterobacter) if it has high acid that would appear a dark shiny green and would be E-Coli
If it doesn't do lactose fermintation it would have no beta galactidase examples would be salmonella, shigella & yersinia pestis
Blood agar (enrichment)
Differential based on hemolysis
ALPHA HEMOLYSIS
" partial/ incomplete hemolysis"
Example streptococcus mutans
& streptococcus pneumoniae
B-BETA HEMOLYSIS
Complete hemolysis/ destruction
Examples: staphylococcus aureus, streptococcus pyogenes, streptococcus agalactiae
Cell wall types:
Group A streptococcus (GAS) contains streptococcus pyogenes
Group B streptococcus (GBS) contains streptococcus agalactiae which is neonatal sepsis
GAMMA HEMOLYSIS
Is no hemolysis
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