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83 Cards in this Set
- Front
- Back
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phenol red |
What is the pH indicator in a TSI? |
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Ferrous sulfate
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What is the indicator of H2S in a TSI?
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Glucose
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What carbohydrate has been fermented in this TSI?
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Sucrose and lactose have not been fermented
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What does the slant of this TSI tell you about the fermentation of carbohydrate?
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Sucrose and/or lactose have been fermented
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What does the slant of this TSI tell you about the fermentation of carbohydrate?
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Acid
Ferrous sulfate will not turn black in the presence of H2S unless both H2S and acid are present, so the black color can be read as H2S positive and acidic. |
What is the pH of the butt of this TSI? How do you know?
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In some organisms, glucose fermentation produces gas (CO2)
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Why is gas produced in TSI by some organisms?
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Alkaline
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What is the pH of this TSI slant?
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Ammonia
Deamination of the amino acids of protein in the media |
What is produced to turn this TSI slant this color? How is this product made?
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Pseudomonas is an aerobe and thus can not ferment any carbohydrates. The slant turns red/pink because of protein utilization (deamination)
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Why does Pseudomonas not turn a TSI yellow?
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K slant
A butt Gas negative Sulfur positive |
What are the results of this TSI?
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K slant
A butt Gas positive (small bubble on side) Sulfur negative |
What are the results of this TSI?
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A slant
A butt Gas negative Sulfur positive |
What are the results of this TSI?
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A slant
A butt Gas positive Sulfur negative |
What are the results of this TSI?
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phenol red
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What is the pH indicator in a carbohydrate fermentation broth?
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Acidic (A)
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What is the pH of this carbohydrate fermentation broth?
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Alkaline (K)
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What is the pH of this carbohydrate fermentation broth?
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Neutral (N)
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What is the pH of this carbohydrate fermentation broth?
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Acidic with gas (AG)
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What is the pH of this carbohydrate fermentation broth?
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Ammonia
Deamination of the amino acids of protein in the media |
What is produced to turn this carbohydrate fermentation broth red/pink? How is this product made?
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Lactose
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What is the carbohydrate in a MacConkey plate?
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Neutral red
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What is the pH indicator in a MacConkey plate?
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Bile salts and crystal violet
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What are the inhibitors in a MacConkey plate?
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Acid
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What is produced when lactose is fermented in a MacConkey plate?
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acid
lactose |
The bright pink colonies on this MacConkey plate indicate that ____ is produced when _____ is fermented.
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lactose
protein |
These translucent pale pink colonies on this MAC indicate that ____ was not fermented but ____ was used as a carbon source instead.
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positive
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This MacConkey plate is lactose ______(+/-). |
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negative
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This MacConkey plate is lactose ______(+/-). |
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bile salts
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The pink fuzzy halo around these colonies growing on a MacConkey plate is the result of lactose fermentation and the precipitation of ________ because of the very low pH.
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Reversion |
What causes the results of lactose positive organism on MacConkey's to appear to change to negative? Why? |
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Gram positives |
MAC is selective because bile salts and crystal violet inhibit the growth of _____. |
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Lactose
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What is the carbohydrate in EMB?
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Eosin and methylene blue
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What is the pH indicator in EMB?
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Eosin and methylene blue
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What are the inhibitors in EMB?
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Acid |
What is produced when lactose is fermented in EMB?
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acid |
The dark red/maroon colonies on this EMB plate indicate that ____ is produced when _____ is fermented.
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lactose |
These translucent pale pink colonies on this EMB indicate that ____ was NOT fermented but ____ was used as a carbon source instead. |
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acid
lactose |
The colonies that are dark red/maroon with a green-black metallic sheen on this EMB plate indicate that a lot of ____ is produced when _____ is fermented. |
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positive
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This EMB plate is lactose ______(+/-). |
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negative |
This EMB plate is lactose ______(+/-). |
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Gram positives
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EMB is selective because eosin and methylene blue inhibit the growth of _____.
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Serratia
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This red pigment indicates that this organism is _____
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Pseudomonas
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Which organism smells like grapes?
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Proteus
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The spreading growth on this TSA indicates growth of ______
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Swarming
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The spreading growth on this TSA is called ______.
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Enterobacteriaceae
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The family that enterics belong to is the _____
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Facultative aerobes
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All enterics are _____ (O2 utilization)
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Glucose
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Which carbohydrate can all enterics utilize?
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Mannitol
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What is the carbohydrate in MSA? |
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Phenol red
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What is the pH indicator in MSA?
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7.5% NaCl
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What is the inhibitor in MSA?
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Acid
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What is produced when mannitol is fermented in MSA?
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acid
mannitol |
The bright colonies with a yellow halo on this MSA plate indicate that ____ is produced when _____ is fermented. |
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mannitol
protein |
These white colonies with a bright pink halo on this MSA indicate that ____ was NOT fermented but ____ was used as a carbon source instead. |
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positive
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This MSA plate is mannitol ______(+/-). |
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negative
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This MSA plate is mannitol _____(+/-). |
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Non-halophiles
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MSA is selective because the high salt inhibits the growth of _____
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Staphylococcus
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The organism in our lab that grows on MSA because it is a halophile is _____
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Ammonia
Deamination of the amino acids of protein in the media |
What is produced to turn this MSA bright pink? How is this product made?
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positive
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This MacConkey plate is lactose ______.
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Sodium thiosulfate
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What is the sulfur source in a TSI?
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Acid |
What is the pH of the butt of this TSI? |
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Selective |
What type of media is NaCL? a. selective b. differential c. nutrient d. selective an differential |
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Non-halophiles |
What organisms are are inhibited on NaCl agar? |
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7.5% NaCl |
What is the inhibitor in NaCl agar? |
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halophiles |
What type of organisms can grow on NaCl media? |
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selective |
What type of media is phenylethyl alcohol agar? a. selective b. differential c. nutrient d. selective an differential |
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selective |
What type of media is crystal violet agar? a. selective b. differential c. nutrient d. selective an differential |
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Gram positive |
What organisms are are inhibited on crystal violet agar? |
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gram negatives Proteus will not swarm |
What organisms are are inhibited on phenylethyl alcohol agar? |
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inhibitor |
The NaCl in NaCl agar is the ____. |
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inhibitor |
The crystal violet in crystal violet agar is the ___. |
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inhibitor |
The phenylethyl alcohol in PEA is the _____ |
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Gram positives |
What type of organisms can grow best on PEA media? |
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Gram negatives |
What type of organisms can grow best on crystal violet media? |
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It causes the outer membrane of gram negatives to leak potassium which disrupts DNA synthesis |
How does the inhibitor phenylethyl alcohol kill gram negative organisms? |
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left |
Which side of the plate is an organism that is starch negative? |
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right |
Which side of the plate is an organism that is starch positive? |
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amylase |
What enzyme causes starch hydrolysis? |
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maltase |
What enzyme converts maltose to glucose? |
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maltose |
Amylase converts starch to dextrans and then to ____. |
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iodine |
What is the indicator added to starch agar? |
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blue-black/ |
What color does iodine turn in the presence of starch? |
