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6 Cards in this Set

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Structure and importance of plasma membranes inside and around cells.

1. description of phosphlipid bilayer + diagram - control movement of substances into and out of cells


2. fluid-mosaic


3. contains channel proteins, pumps, in- and extrinsic proteins, cholesterol to make membrane fluid


4. thylakoid membranes - LDR photosynthesis - large SA for attachment of chlorophyll, enzymes and electron carriers


5. inner mitochondrial membranes - ETC


6. prokaryote cell membrane


--> mesosome


--> formation of pilus (conjugation) for horizontal gene transmission


7. neurones - AP - gated ion channels and pump in axon membrane


8. synapses


9. small intestine epithelial cell membranes contain Cl- and Na+ channel proteins - take ions in from lumen to maintain water potential gradient


10. mRNA - fitting through nuclear pore to attach to ribosomes on RER


11. sperm + egg membrane fusion - sperm releases digestive enzymes which degrade the layer of glycoproteins on the egg, allowing the membranes to fuse - sperm nucleus enters egg


12. alkaline hydrothermal vents - have membranes containing H+ - thought to be how life started - form acetyl phosphate and pyrophosphate

Carbon dioxide may affect organisms directly or indirectly. Describe and explain these effects.

1. Respiring tissues produce more CO2 - lower pO2 - Hb unloads O2 used in repiration


2. chemoreceptors in aorta and carotid arteries - CO2 to carbonic acid - lowers pH - impulses to medulla oblongata - increases ventilation rate and heart rate


3. needed in LIR photosynthesis - RuBP + CO2 --> GP --> TP --> glucose


4. indirect - glucose from LIR used in respiration


5. carbon cycle - C compounds from plants and animals - decomposers - that which isnt respired is fossilized - fossil fuels combusted


6. rising CO2 in atmosphere - 40% more since before industrial revolution - climate change


--> higher crop yields


--> rising temps, more unstable weather - affects distribution of animals and plants - mosquitoes of the Anopheles genus move further north - higher prevalence of malaria


--> warmer oceans - photosynthetic zooxanthellae algae in corals leave/die - bleaching - corals weaker


--> more CO2 in oceans - acidification - CaCO3 reacts with acid - damages coral skeleton + clam, snail, urchin shells - can't absorb more to maintain

Structure of cells related to function.

1. chloroplasts


--> thylakoid membranes - large SA for attachment of chlorophyll + enzymes + e- carriers for LDR


--> starch grains - efficient storage


2. guard cells - take in water when light - become turgid - opens stomata


3. prokaryotes


--> flagella - needed for Vibrio cholerae bacteria have one to propel them through mucous lining of small intestine


--> plasmids - horizontal gene transmission - form a pilus for conjugation


4. muscle fibre cells - no membranes between them (multinucleate) - contain actin and myosin for muscle contraction


5. cells in liver, muscle, brown fat - lots of mitochondria for respiration


6. neurones - axons contain voltage-gated ion channels and pump in membrane - myelinated and unmyelinated


7. small intestine epithelial cells have villi and microvilli for large SA for increased absorption


8. epithelial cells in alveoli + capillaries - flat - efficient gas exchange


9. root hair cells - large SA for osmosis and active transport of mineral ions


10. red blood cells


--> biconcave - flexible, can fit through capillaries


--> contain Hb


--> no nucleus, can carry more Hb, carry more O2


11. phloem - sieve cells separated with perforated sieve plates


12. xylem - no membranes between cells, no organelles


13. fungal cells have a cell wall made of chitin that protects the cell

The biological importance of water.

1. H20 - delta +ve and -ve - allows hydrogen bonds - makes is cohesive + adhesive - sticks to charged surfaces like xylem vessel


2. transpiration - water in xylem and phloem sap carry sugars and salts


3. blood plasma - carries red blood cells, glucose, CO2, proteins


4. O2 - terminal e- acceptor in ETC - forms H2O


5. sweat - cools you down, prevents hyperthermia


6. taxes and kinesis


7. substrate for LDR photosynthesis


8. antibiotics cause bacterial cell walls to not form properly - water moves down WP gradient by osmosis - osmotic lysis


9. amphibians need to return to water to breed

Using DNA in science and technology.

1. structure of DNA - differences lead to genetic diversity


2. In Vivo - producing DNA fragments (restriction endonucleases, reverse transcriptase) - plasmid vectors, marking


3. In Vitro - PCR


4. Genetic screening - Sanger sequencing


5. Taxonomy - base sequence + amino acid compared


6. cell cycle + treatment of cancer


7. gene therapy - current techniques--> gene replacements--> inactivating mutated gene--> repairing gene (selective reverse mutation)--> gene supplementation - adding healthy dominant gene, masks effect of faulty gene - used to alleviate effects of CF - inhaler or nasal spray--> somatic + germline


8. possible future techniques - CF and SCID


9. GM organisms


--> plants - tomatoes - don't go soft


--> animals - produce anti-thrombin in milk


10. ethical issues of gene therapy and GM

The importance of shapes fitting together in cells and organisms.

1. complementary bases


--> DNA replication


--> taxonomy - comparing base sequences


--> use of probes in genetic screening


2. enzyme active sites


3. enzymes inhibition


--> can be used as treatment - statins


4. e.g. rubisco


5. glucagon receptor sites in liver cells - cAMP - glycogenolysis


9. muscle contraction - Ca binding to troponin, myosin head binding sites


10. haemoglobin and O2


11. translation - anticodons and AAs