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10 Cards in this Set

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5pts 2of4
2a) Glucose into triglyceride in MAMMALS.
----- Substrates required for triglyceride synthesis? -----
1)

----- Pathways for their synthesis-----
__ *** __
1)
2)
3)

__ *** __
1)

----- Final Reaction -----
1)
2a) Glucose into triglyceride in MAMMALS.
----- Substrates required for triglyceride synthesis? -----
1) Fatty Acyl-CoA + Glycerol Phosphate

----- Pathways for their synthesis-----
__ Fatty-Acyl-CoA __
1) glucose [glycolysis] pyr [PDC] Acetyl-CoA
2) Acetyl-CoA + HCO3- [ACC] Malonyl-CoA
3) Acetyl-CoA + Malonyl-CoA [FAS] Fatty-Acyl-CoA

__ Glycerol Phosphate __
1) glucose [glycolysis] DHAP [GPDH] Glycerol Phosphate

----- Final Reaction -----
1) Fatty Acyl-CoA + Glycerol Phosphate [Acyltransferase] Triglyceride
2b) 5pts 2of4
What compound? What C is tagged?
1) Fructose/Glucose (C-3) --- Mammalian --- Anaerobic
2) Pyruvic acid (C-3) --- Pyruvate Dehydrogenase --- Anaerobic
3) Palmitic Acid 16:0 (C-1) --- β-Oxidation Pathway--- Anaerobic
4) Fructose/Glucose (C-3) --- Alcohol Ferm --- Anaerobic
1) C-1 of lactate
2) No metabolism --- C-3 of Pyruvic
3) No metabolism --- C-1 of Palmitic Acid
4) CO2
5pts 2of4
2c)
------ What are the Aspartate-Family AAs? ------
1)

------ Glucose into Asp-Family AAs in PLANTS ------
1)
2)
3)
4)

------ Starting w/Sucrose? ------
1)
------ What are the Aspartate-Family AAs? ------
1) lysine/threonine/methionine

------ Glucose into Aspartate-Family AAs in PLANTS ------
1) Glucose [glycolysis]
2) pyr [GNG]
3) OAA [transaminase]
4) Asp [Asp-Family AA Synthesis Pathway] lys/thr/met

------ Starting w/Sucrose? ------
1) Sucrose [invertase] GLUCOSE + fructose
2d) 5pts 2of4
Maximum ATP generated AEROBICALLY from...
1) Pyruvic Acid - Mammal
2) 20:0 Fatty Acid
3) Glucose - Skeletal Muscle - Glycerol-Phosphate Shuttle
4) 18:0 Fatty Acid
5) Glucose - From Glycogen - Malate-Aspartate Shuttle
1) 12.5
2) 134
3) 30
4) 120
5) 33
5pts
2cextra)
------ Alanine into Glycogen ------
1)
2)
3)
4)
5)
1) Alanine [transaminase]
2) pyr [GNG]
3) OAA [GNG]
4) Glucose-6-P [PhosphoGlucoMutase]
5) Glucose-1-P [Glycogen Synthesis] Glycogen
5pts
2aextra)
------ Citrate into Palmatate 16:0 ------
1)
2)
3)

--- Final Reaction ---
1)
------ Citrate into Palmatate 16:0 ------
1) Citrate [Citrate lyase]
2) OAA + Acetyl-S-CoA (+ HCO3) [ACC]
3) Malonyl-S-CoA [GNG]

--- Final Reaction ---
1) Acetyl-S-CoA + Malonyl-S-CoA [FAS] Palmatate (16:0)
2dextra) 5pts 2of4
Steps of ATP generation from 20:0 Fatty Acid (Aerobic)
--- Overall ---

--- Specifics ---
Activation = -2
Shuttle = 0
β-Oxidation = 136
TOTAL = 134

--- β-Oxidation ---
[X/2-1] NADH (9) = 22.5
[X/2-1] FADH2 (9) = 13.5
[X/2] Acetyl-CoA (10) = 100
--- Glucose Metabolism ---
How many Steps?
Name the Steps/ATP.....
5

1) --- From Glycogen (+1)
2) --- Glycolysis (+7)(A,A,N,N)
3) --- Shuttle System ---
Glycerol-Phosphate Shuttle (-2)
Malate-Aspartate Shuttle (+0)
4) --- Pyruvate Decarboxylase Complex (+5)(N,N)

5) --- TCA (+20)
(N,N,N)(N,N,N)
(G,G,F,F)
--- Pyruvate Metabolism ---
How many Steps?
ATP Yield?
Name the Steps/ATP.....
2 Steps
12.5 ATP

1) Pyruvate Decarboxylase Complex (+2.5)(N)
2) --- TCA (+10)(N,N,N,G,F)
--- Compare / Contrast Mitochondrial and Chloroplast E.T. Pathways ---
Mitochondrial --- Chloroplast
1) # of C-subunits
2) The *** space is interior.
3) H+ is forced from the *** space into the ***
4) # protein complexes
5) 1st & 2nd protein complexes linked by...
6) 2nd & 3rd protein complexes linked by...
7) Generates initial e- from...
1) 12humans/10yeast --- 14
2) Matrix --- Thylakoid lumen
3) Matrix/INTERmembrane ---Stroma/Thylakoid lumen
4) 3 --- 3
5) ubiquinone --- plastoquinone
6) cytochrome c --- plastocyanin
7) NADH --- light