Blood Culture Contamination For the purpose of this research, Archbold Medical Center Laboratory is the place I chose to conduct my research. Blood Culture contamination is a big problem not only at Archbold, but at many hospitals. The problem is not only within the laboratory, but from outside sources also. Although contamination is a problem, it can be easily fixed. Whenever a blood culture is ordered by a physician, we can assume the patient is experiencing some type of bacterial infection…
To begin, a streak was performed on two TSA plates and a blood agar plate using the three-sector streak technique from the unknown culture tube to isolate the two unknown bacterial colonies. The plates were then incubated for 12 hours at 37 ̊C. The blood agar plate was used to create a greater contrast in color to help distinguish between bacterial colonies by size and color. Two distinct colonies could be seen growing on the blood agar plate shown in figure 1. The smaller, opaque colored…
Objectives: The purpose of this project is to identify two separate microbial organisms from a given list, which are to be inoculated from a mixed broth culture. Multiple tests will be performed in order to eliminate all organisms from the list that do not provide significant results for the identification of the unknown specimens. Journal Entries: April 17, 2018. Today we began the unknown project. I obtained the unknown broth tube #15. I used two Tryptic Soy Agar (TSA) plates in order to…
Blake Smith Mr. Engstrom Physics May 8, 2017 Eta Carinae The Eta Carinae, previously known as Eta Argus, has a luminosity five million times greater than the suns. The Eta Carinae has two stars and for examples we will name them η Car 1 and η Car 2. η Car 1 has a mass of about ~100 to 200 solar masses, which equals about 1.99 × 1030 in kilograms. η Car 1 also has a 60 to 800 solar radius, which is 6.957 00 × 108 meters. η Car 1 has a 5,000,000 Luminosity. η Car 1 has a temperature of 9,400…
colonies as well as clusters. The clusters were moist in consistency as well as opaque and dense. The growth followed the pattern of the smear, leaving some portions of the growth to appear as pseudofilaments. The microbe’s growth also grew into the medium about a fifth of the way into the agar. The E. coli showed as cloud and fluffy like segments suspended in the nutrient broth. Section B of the controlled agar tray, M. luteus, colonized in single colonies that were very close in arrangement.…
Day 1: On the first day of testing the unknown bacteria, B7 was aseptically inoculated in four different ways. The first procedures that were done was the inoculation of a nutrient broth tube, nutrient agar slant, and a nutrient agar plate. These three inoculations are all done with a loop. The first of these three procedures is the inoculation of B7 into the nutrient broth agar. First, the tip of the loop is flamed in the blue cone of the Bunsen burner at a 45-degree angle to sterilize and prep…
In order to perform this experiment, we had to follow the general directions to create our steps for the procedure of the lab. We first set up our habitat for the bacteria to grow and reproduce, which in this case was agar within a petri dish. After dividing and labeling said petri dish into four quadrants with a Sharpie on the bottom piece, it was time to add the microorganisms into the labeled petri dish quadrants. Once we swiped the bacteria-coated QTip across each area— three times per…
paragallinarum reference strains (HP1, HP 90 and HP144) and two Australian Av. paragallinarum reference strains (HP14 and HP60) were selected for growth curve construction. Three broths were tested for their suitability of growth, Haemophilus Test Medium Broth (TMB), Cation-adjusted Mueller-Hinton Broth (CAMHB) with supplements and Veterinary Fastidious Media (VFM). TMB was prepared with 1% Biosate Peptone (BD), 1% sodium chloride (Merck Millipore, Australia), 0.1% starch (Merck Millipore), 0.1%…
Oral Microbiome 10: Reflection 3 One a daily basis the human mouth can come in contact with many different materials and organisms. “The human mouth is home to billions of individual microorganisms, including viruses, protozoa, fungi, archaea, and bacteria” (University of Minnesota Department of Biology Teaching and Learning, 2016, p. 21). In specific, during this lab we are looking at Streptococcus mutans (S. mutans) and Lactobacilli. In studies, it has been shown that yogurt has helped…
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