First the unknown tube was isolated. This was completed by taking an inoculating loop and spreading the unknown out on to a Nutrient Agar plate. This was done using the streak plate method, which is described in the lab manual. The bacterium were incubated and grown at 48 hours. Upon observation the isolation plate showed growth of two different colonies. After the morphology was observed and recorded, a Gram stain was performed on each of the two unknowns, this procedure is found in the…
It is really easy to blame your financial difficulties on money. Yet what some people forget, is that this medium of exchange allowed us to create what we have today. Without something to use as currency there would be no society. A society consists of a group of people working together to create an orderly community. The creation of which would not be possible if the basic things that humans need are not satisfied. Money helps create stability by setting a certain value on all the commodities…
Media is a dynamic institution that reflects the needs, wants, and desires of society. It is constantly developing in rhythm with humans over time to cater to our informative needs. The creation of the internet, then cellphones, and social media portrays the rapid growth and changes of the institution. The 21st century is rapidly advancing and therefore, we cannot guarantee its future but we can predict it. The theories of the ‘hot and cool media’, ‘the global village’, and the ‘narcissism…
When it derives to contemplating out an unknown bacterial species the whole purpose is to benefit us in order to better understand what this species is capable of. Throughout a general basis of identifying an unknown species we must figure out either it will become a gram-positive or gram-negative type of bacteria and in doing so we must do a gram staining method. This method works because it will direct us to what test we can do next based off either it is negative or positive bacteria, which…
2.5. ANTIOXIDANT STATUS 2.5.1. TLC - Semi Quantitative DPPH Assay 0.2 % DPPH solution in methanol was prepared and kept in the fridge for further use. The grid space was marked with 1.0 cm2 space on an aluminum based TLC sheet (Merck silica gel 60F254) and a stock solution of all the extracts together with the standard were prepared in methanol. A series of dilutions of the stock together with the standard were prepared ranging from 400 µL to 0.01µL for the last dilution. The grid on the TLC…
Blood Culture Contamination For the purpose of this research, Archbold Medical Center Laboratory is the place I chose to conduct my research. Blood Culture contamination is a big problem not only at Archbold, but at many hospitals. The problem is not only within the laboratory, but from outside sources also. Although contamination is a problem, it can be easily fixed. Whenever a blood culture is ordered by a physician, we can assume the patient is experiencing some type of bacterial infection…
Blake Smith Mr. Engstrom Physics May 8, 2017 Eta Carinae The Eta Carinae, previously known as Eta Argus, has a luminosity five million times greater than the suns. The Eta Carinae has two stars and for examples we will name them η Car 1 and η Car 2. η Car 1 has a mass of about ~100 to 200 solar masses, which equals about 1.99 × 1030 in kilograms. η Car 1 also has a 60 to 800 solar radius, which is 6.957 00 × 108 meters. η Car 1 has a 5,000,000 Luminosity. η Car 1 has a temperature of 9,400…
To begin, a streak was performed on two TSA plates and a blood agar plate using the three-sector streak technique from the unknown culture tube to isolate the two unknown bacterial colonies. The plates were then incubated for 12 hours at 37 ̊C. The blood agar plate was used to create a greater contrast in color to help distinguish between bacterial colonies by size and color. Two distinct colonies could be seen growing on the blood agar plate shown in figure 1. The smaller, opaque colored…
Objectives: The purpose of this project is to identify two separate microbial organisms from a given list, which are to be inoculated from a mixed broth culture. Multiple tests will be performed in order to eliminate all organisms from the list that do not provide significant results for the identification of the unknown specimens. Journal Entries: April 17, 2018. Today we began the unknown project. I obtained the unknown broth tube #15. I used two Tryptic Soy Agar (TSA) plates in order to…
these shots, like in shots 41 and 51, the car is moving from frame left to right each time. Without a sense of continuity of direction, the viewer would not know where the car was headed, and if it was making any progress. By showing the repetition of the car heading from left to right, we get a sense that we are getting closer to the goal, which will eventually be on the right side of the frame. This was especially important in Death’s Marathon, since the car was in such a rush, the shot…