Gram-negative bacteria

not affect the pH of the orange juice, but it could be possible that the extracts maintained the pH of the juice and hence, limiting the growth of some microorganism. The minimum pH for the growth of yeast and mould is 1.5, whereas for the aciduric bacteria is between pH 2.9-4.5 (Aneja et al 2014). This show that the low pH environment of the juice was not low enough to eliminate the growth of…

β-mercatpoethanol (mol. wt. 78.13) from LOBA Chemie, sodium dodecyl sulfate (SDS) (mol. wt. 288.38) and urea (mol. wt. 60.06) was purchased from Merck, and ethanol was purchased from Sigma-Aldrich. Antimicrobial strain of gram negative bacteria Escherichia coli (E. coli) [MTCC 118] and gram positive bacteria Bacillus subtilis (B. subtilis) [MTCC 121] was purchased from MTCC Chandigarh, India. 2.1. Fabrication of Porous Scaffold from Keratin/Alginate binary blend: The extraction of keratin from…

Introduction Gram staining is a method commonly used to determine the chemical make up of the cell wall of bacteria. The cell wall can stain either positive or negative, depending on its chemistry.The aim of this experiment. Gram staining was the first differential technique used and still in use to make microscopic vision of bacteria clearer while also sub-divides the bacteria into two major groups: Gram-positive and Gram –negative. This experiment should help us describe how Gram staining…

between gram negative and gram positive bacterium. This is important when doing gram staining experiments. It also looks at the importance of microbiology. DIFFERENCES OF GRAM NEGATIVE AND GRAM POSITIVE BACTERIUM There are notable differences between the gram negative and gram positive bacterium. Gram negative bacterium is surrounded by a thin peptidoglycan cell wall while Gram positive has a thick layer of peptidoglycan. The peptidoglycan around the gram negative is about 8-10 mm while in gram…

I. Purpose: The purpose of this lab was to learn how to properly mount a bacteria for examination under a microscope. Also learned in this lab were methods on how to inoculate an agar or broth medium, and using aseptic techniques. Lastly, how to use stains to visualize bacterial cells, or distinguish different types of cells from one another through the usage of dye techniques such as the Gram-stain test was learned. II. Prepping/Mounting/Simple Staining Bacterial Cells Procedure: 1. Obtain 3…

Contamination by Site While the previous data set is useful in showing the overall prevalence of pathogenic microbes on computers, it may be useful to further examine variations in contamination levels between different departments. This would allow one to determine which departments face the most risk and require the most changes. In the study conducted by Po-Liang et al. (2009), no significant differences in pathogenic contamination rates between ICU workstations and non-ICU workstations (7.4%…

consisted of two unknown bacteria, Gram-positive or Gram-negative that were streaked from a pure culture. Sterile techniques were followed while performing precise instructions as stated in the referenced Laboratory Manual. Example 1: The first procedure performed was done by isolating a pure culture from the mixture onto a solid Trypticase soy agar (TSA) media. Sterile technique was done by flaming the loop until it turned red to ensure that there were no current bacteria on the loop avoiding…

INTRODUCTION There have been multiple staining techniques before the Gram stain came about in 1883. These old staining techniques brought about issues because they stained both the tissue and the cells so it was hard to differentiate between the two. Christian Gram is the inventor of the gram stain and found this technique when he was looking at Streptococcus pneumoniae in the lung tissue of people and animals (1). When working with these tissues, he found that this bacterium would take up a…

an unknown bacteria by using first the Gram Stain and based on the results of the gram staining, several biochemical tests can be performed to identify with accuracy the unknown bacteria. II. Identification Bacteria are divided into Gram Positive and Gram Negative based on the chemical composition of the cell wall. Gram stain, a differential stain, can be used to identify the shape, arrangement, and chemical composition of the microorganisms. In the chemical composition of the Gram stain, we…

Desulfovibrio is gram negative bacteria. There are media that will be useful in isolating this organism. The best option for a gram negative bacteria would be using a selective media specifically MacConkey agar or EMB agar. These agars are used to isolate gram negative bacteria because the crystal violet in the MacConkey agar will not allow the growth of gram positive bacterium making it a good media to isolate Desulfovibrio. If that selective media does not work then another media is to use a…