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55 Cards in this Set
- Front
- Back
What is meant by the statement: "microbes are ubiquitous"?
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they are everywhere
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What is Microbiology?
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The study of
microscopic organisms |
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At the beginning of the 19th century, microorganisms were considered what?
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Mysterious oddities
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When did the world become aware of the effects of microorganisms?
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The 19th century
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How are Microorganisms harmful to humans?
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Agents of food spoilage
Agents of disease |
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How are Microorganisms useful to humans?
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They are involved in the study of various processes of living systems.
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How are Microorganisms beneficial to humans?
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They are decomposers of dead organic matter and are essential for food formation (cheese, yogurt).
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"Etiological agents of disease" means what?
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Etiological means 'origins of', and the statement refers to the 'microbial causes of disease'.
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Microbes which cause disease are called what?
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Pathogens
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What fields of science have benefited from microbiology?
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Biochemistry and Genetics are 100 yrs behind without their study.
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Name the things microbes manufacture which are beneficial to humans:
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antibodies, hormones, amino acids, vitamins, interferons, and contribute to growth factors.
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What are interferons involved in?
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Interferons are involved in the treatment of cancer
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How are microbes harmful?
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They spoil food and they can be pathogenic
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T/F
microbes are beneficial to humans in that they manufacture proteins |
true
they manufacture amino acids |
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What is the theory of Spontaneous Generation?
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the idea that life could and did appear spontaneously from nonliving and decomposing matter (ie frogs from mud, flies from rotting meat)
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Who was creidited with the formation of the Spontaneous Generation Theory?
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Aristotle (346 BC) is credited with the theory
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Who was the first to discredit the theory of Spontaneous Generation?
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Francisco Redi (1665)
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How was the theory of Spontaneous Generation discredited?
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Redi's experiment showed that flies were not spontaneously generated in meat: that they came from eggs (maggots).
Experiment involved two bottles with meat inside: one with netting, the other uncovered. The uncovered one showed flies, the other none. |
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Who is considered the 'Father of Microbiology'?
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Antonie van Leeuwenhoek
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What did van Leeuwenhoek do?
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He was the first to gaze upon Euglena, discovering bacteria and describing microscopic forms.
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What did Leeuwenhoek use in his research?
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He used a microscope of his own design, which magnified up to 500 times.
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Who postulated the "Germ Theory of Fermentation"?
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Louis Pasteur
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What is the "Germ Theory of Fermentation"?
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"A natural process in which alcohol and organic acids are formed from dissolved sugar in the presence of microorganism and the absence of air."
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Describe the "Non-vital" viewpoint on fermentation
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yeasts is a byproduct of fermentation
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Describe the "Vital" (biological) viewpoint on fermentation
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fermentation is a byproduct of the yeast
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Joseph Lister (English) did what?
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developed the antiseptic surgery sterilization of instruments and application of carbonic acid (phenol) to wounds by means of dressing
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The Compound Microscope:
Magnification |
the ability to enlarge image
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The Compound Microscope:
Resolution (or Resolution Power) |
the ability to show detail clearly
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formula for Resolution Power
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RP = wave length of light (in nm)
_________________________ 2x numerical appeture |
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white light in nm
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between 380nm (violet) and 750nm
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resolution is the function of
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Resolution is the function of the wave length of light
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What are the levels of classification in taxonomy?
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Kingdom
Philum Class Order Family Genus Species |
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Taxonomy is ...
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the science of classification
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how many different species are named and described?
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1.8 million
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what is classification?
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the arrangement of organisms according to their properties and characteristics
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binomial system of nomenclature:
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each organism has two word names
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What other four scientists before Pasteur have disproved spontaneous generation (that we have studied)?
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Francisco Redi
Louis Jabolt Theodore Schwann Franz Schulze |
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What are the steps to the Nitrogen Cycle
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1. Nitrogen Fixation
2. Nitrification 3. Assimilation 4. Ammonification 5. Denitrification |
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Nitrogen Cycle:
Nitrogen Fixation |
Converts atmospheric nitrogen (n2) into ammonia (NH3)
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Nitrogen Cycle:
Nitrification |
Conversion of Ammonia (NH3) into Nitrate (NO3-)
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Nitrogen Cycle:
Assimilation |
the use of ammonia (NH3) and Nitrate (NO3-)by plants to make their own proteins and bodies, then eaten by animals
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Nitrogen Cycle:
Ammonification |
conversion of organic compounds and wastes into ammonia
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Nitrogen Cycle:
Denitrification |
conversion of nitrate (NO3-) into free nitrogen (N2)
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What are the steps involved with nitrification?
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1. ammonia (NH3) to Nitrite (NO2-) with the help of the bacteria nitrosomonas
2. from Nitrite (NO2-) to Nitrate (NO3-) with the help of bacteria Nitrobacter |
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Give an example of ammonification
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Urea is converted by bacteria Proteus Vulgaris into ammonia during urea hydrolysis
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give an example of denitrification
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in the lab, tests are done -- called Nitrate reduction -- where bacteria can convert Nitrate (NO3-) to Nitrite (NO2-) [e.coli] or Nitrate to free nitrogen (n2) [pseudomonas]
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Louis Jablot
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1711
showed microbial growth when sterilized soup is uncovered vs covered (stoppered tightly after boiling) |
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Pasteur's quote re swan neck experiments
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"For I have kept from them, and am still keeping from them the one thing which is above power of man to make: I have kept from them the germs that float in the air, I have kept from them life."
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The GERM THEORY OF FERMENTATION
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fermentation is a natural process in which ALCOHOL AND ORGANIC ACIDS are formed from dissolved sugar in the presence of microorganisms and the absence of air
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first proof that bacteria cause disease
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Rober Koch in 1876 found rod-shaped bacteria in the blood of cattle that died of anthrax.
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Koch's postulates
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1. The organism must be found in all animals suffering from the disease, but not in healthy animals.
2. The organism must be isolated from a diseased animal and grown in pure culture. 3. The cultured organism should cause disease when introduced into a healthy animal. 4. The organism must be reisolated from the experimentally infected animal. |
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Gelatin melts at
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26 deg C
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who introduced the Petri Dish?
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Richard Petri, assistant to Koch
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Walter Hesse did what?
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assistant to Koch, he learned of agar-agar as a better solidifying agent than gelatin
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agar solid at what temps?
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solidifies from 32-40 °C.
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