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28 Cards in this Set

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What is the name of the GNR confirmatory test that has 4 different things to be read/interpreted?
TSI - Triple Sugar Iron
How is a TSI set up?
Innoculate the media w/ single colony to w/i 1/2 inch of the bottom and streak the wire up the slanted part of the agar. Cap loosely and incubate for 18 to 24 hours.
How are TSI results reported?
slant/butt/gas/H2S
TSI butt - how read and recorded?
read as a color change - reported as "A" (acid) if yellow, "K" (alkaline) if red
TSI slant - how read and recorded?
reported as a color change - recorded as "A"(acid) for yellow and "K" (alkaline) for red
TSI gas - how read and recorded?
Read as tears or bubbles in the agar - recorded as + or -
TSI H2S - how read and recorded?
read as the presence or absence of a black color along the stab line - recorded as + or -
The black color from a + H2S may occassionally masks the color of the butt - if this happens how should it be recorded and why?
Recorded as yellow,"A"(acid) since all organisms that produce H2S also ferment glucose.
What three things are interprted w/ a SIM test?
H2S, indole, motility
How is a SIM test set up?
Innoculate the agar w/ single colony - stab to w/i 1/2 inch of the bottom. Cap loosely and incubate for 24 to 48 hours.
How is H2S read and recorded on a SIM test?
black color is positive, no black color is negative
How is motility read and recorded on a SIM test?
cloudy agar is +, clear agar (stab line is clearly visible) is -
After reading the H2S and motility on a SIM test, how is indole read and recorded?
Add 2-3 drops of indole reagent to the tube and observe for color change. Red color development w/i 10 seconds is a +, no red is a -.
How is a Simmons Citrate test set up?
Slant the slant with a wire, incubate with a loose cap
How is a Simmons Citrate test read and recorded?
If agar is blue it is positive, if it is green it is negative.
How is a Phenylalanine test set up?
Streak the slant and incubate w/ a loose cap
How is a phenylalanine test read and recorded?
Add 0.cml of FeCl solution to cover the growth and observe for immediate color change. Green is positive and no green is negative
How is a urea test set up?
Inncoulate the urea broth and incubate over night w/ a loose cap
How is a urea test read and recorded?
read as color change - Pink (bright) is a positive, Salmon is a weak positive, and yellow is negative.
Where is the swab for an oxidase spot test taken? Why?
Taken from BAP - MAC will give a false positive
How is an oxidase spot test done and read/recorded?
Pick up colony off of BAP w/ swab and drop a couple of drops of oxidase reagent on it. A positive test will turn dark purple in 10-20 seconds. A negative test does not develop color.
Pres ID:
Pink colonies on MAC, beta hemolysis, sour milk odor
beta E. coli
Pres ID:
Pink colonies on MAC, gamma hemolysis, sour milk odor
gamma E. coli
Pres ID:
Pink colonies, gamma hemolysis, slimy, mucoid colonies
Klebsiella
Pres ID:
No growth on MAC, gamma hemolysis
pasteurella
Pres ID:
BAP brown, gamma hemolysis, tan colonies on MAC
proteus
Pres ID:
Tan irregular colonies, turns clear agars green, beta/double beta hemolysis, fruity odor, metallic sheen
pseudomonas
Pres ID:
tan colonies, gamma hemolysis, boring
salmonella