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28 Cards in this Set
- Front
- Back
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What is the name of the GNR confirmatory test that has 4 different things to be read/interpreted?
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TSI - Triple Sugar Iron
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How is a TSI set up?
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Innoculate the media w/ single colony to w/i 1/2 inch of the bottom and streak the wire up the slanted part of the agar. Cap loosely and incubate for 18 to 24 hours.
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How are TSI results reported?
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slant/butt/gas/H2S
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TSI butt - how read and recorded?
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read as a color change - reported as "A" (acid) if yellow, "K" (alkaline) if red
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TSI slant - how read and recorded?
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reported as a color change - recorded as "A"(acid) for yellow and "K" (alkaline) for red
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TSI gas - how read and recorded?
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Read as tears or bubbles in the agar - recorded as + or -
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TSI H2S - how read and recorded?
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read as the presence or absence of a black color along the stab line - recorded as + or -
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The black color from a + H2S may occassionally masks the color of the butt - if this happens how should it be recorded and why?
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Recorded as yellow,"A"(acid) since all organisms that produce H2S also ferment glucose.
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What three things are interprted w/ a SIM test?
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H2S, indole, motility
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How is a SIM test set up?
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Innoculate the agar w/ single colony - stab to w/i 1/2 inch of the bottom. Cap loosely and incubate for 24 to 48 hours.
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How is H2S read and recorded on a SIM test?
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black color is positive, no black color is negative
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How is motility read and recorded on a SIM test?
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cloudy agar is +, clear agar (stab line is clearly visible) is -
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After reading the H2S and motility on a SIM test, how is indole read and recorded?
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Add 2-3 drops of indole reagent to the tube and observe for color change. Red color development w/i 10 seconds is a +, no red is a -.
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How is a Simmons Citrate test set up?
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Slant the slant with a wire, incubate with a loose cap
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How is a Simmons Citrate test read and recorded?
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If agar is blue it is positive, if it is green it is negative.
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How is a Phenylalanine test set up?
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Streak the slant and incubate w/ a loose cap
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How is a phenylalanine test read and recorded?
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Add 0.cml of FeCl solution to cover the growth and observe for immediate color change. Green is positive and no green is negative
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How is a urea test set up?
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Inncoulate the urea broth and incubate over night w/ a loose cap
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How is a urea test read and recorded?
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read as color change - Pink (bright) is a positive, Salmon is a weak positive, and yellow is negative.
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Where is the swab for an oxidase spot test taken? Why?
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Taken from BAP - MAC will give a false positive
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How is an oxidase spot test done and read/recorded?
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Pick up colony off of BAP w/ swab and drop a couple of drops of oxidase reagent on it. A positive test will turn dark purple in 10-20 seconds. A negative test does not develop color.
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Pres ID:
Pink colonies on MAC, beta hemolysis, sour milk odor |
beta E. coli
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Pres ID:
Pink colonies on MAC, gamma hemolysis, sour milk odor |
gamma E. coli
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Pres ID:
Pink colonies, gamma hemolysis, slimy, mucoid colonies |
Klebsiella
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Pres ID:
No growth on MAC, gamma hemolysis |
pasteurella
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Pres ID:
BAP brown, gamma hemolysis, tan colonies on MAC |
proteus
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Pres ID:
Tan irregular colonies, turns clear agars green, beta/double beta hemolysis, fruity odor, metallic sheen |
pseudomonas
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Pres ID:
tan colonies, gamma hemolysis, boring |
salmonella
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