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34 Cards in this Set
- Front
- Back
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Areas of bacterial growth on plated media are called
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Colonies
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Bacterial colonies away from, or set apart from other growth or colonies on plated media.
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Isolated Colonies
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Colonies of growth that all run together.
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Confluent growth
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Media that has only one type of bacteria growing in/on it.
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Pure cultures
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Type of media that usually contains protein, salt, dextrose, water, meat extract, and a solidifying agent such as agar or gelatin. It provides the basic requirements for bacteria to grow.
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Basic nutrient media
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BAP and Chocolate are examples of what type of media?
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Enriched
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What type of media is formulated to meet the requirements of fastidious pathogens - are essentially basic nutrient media w/ extra nutrients added such as blood, serum, and egg.
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Enriched media
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What media contains antibacterial substances that inhibit or kill all but a few types of bacteria?
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Selective Media
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MAC, CNA, and Mannitol Salt are examples of what type of media?
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Selective media
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MAC, CNA, and Mannitol Salt are examples of what type of media?
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Selective media
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What type of media allows bacteria to be differentiated into groups by biochemical reactions on the medium?
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Differential media
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MAC, Mannitol Salt and BAP are examples of what type of media?
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differential
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What media is used to differentiate Staph aureus?
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Mannitol Salt
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What type of media are liquids that either contain nutrients that encourage the growth of desired bacteria or contain inhibitory substances that suppress competition?
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Enrichment Broth
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Selenite Broth and Tryptic Soy Broth are examples of what type of media?
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enrichment broth
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What info is recorded on day one of record keeping?
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Source, what plates streaked, and direct gram smear results.
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What info is recorded on day 2 of record keeping?
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what plates showed growth, colony morphology, presumptive id (w/ hemolysis), and set up confirmatories and sensitivity testing
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What info is recorded on day 3 of record keeping?
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Positive ID and sensitivity results
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Why do we do dilution streaking?
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To obtain isolated colonies of pure growth
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4 stains used in Gram staining
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Crystal Violet, Gram's Iodine, Acetone Alcohol, Safranin
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What shows a positive reaction to a Catalase test, Staph or Strep?
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Staph
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What is Catalase +, but Coagulase and mannitol -?
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Staph epi
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What is Catalase, Coagulase, and Mannitol +?
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Staph aureus
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Positive result for Catalase test?
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bubbling
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Positive result for Coagulase test?
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clumping seen in 5-20 seconds
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What is added to bacteria in performing a Catalase test?
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Hydrogen Peroxide
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What is a positive reaction for a Mannitol Salt Agar test?
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Color change in the agar
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On which media is sensitivity testing done?
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Mueller Hinton, except for Strep which is done on BAP
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Positive result for Bacitracin test? Positive result indicates what?
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Any area of growth around disc - Beta-Strep Group A
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Positive result of Optichin test? Positive for what?
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Area of growth greater than 14mm is positive. Strep Pneumoniae.
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How to read test result for NaCl test?
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+ media turns yellow, - remains purple
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How to read test results for Bile Esculin?
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+ media turns black, - remains tan
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What other organism is needed to perform CAMP test other than Strep?
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Staph Aureus
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How to read CAMP test results?
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+ if there is an arrowhead area of growth, - if no arrowhead
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