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34 Cards in this Set

  • Front
  • Back
Areas of bacterial growth on plated media are called
Bacterial colonies away from, or set apart from other growth or colonies on plated media.
Isolated Colonies
Colonies of growth that all run together.
Confluent growth
Media that has only one type of bacteria growing in/on it.
Pure cultures
Type of media that usually contains protein, salt, dextrose, water, meat extract, and a solidifying agent such as agar or gelatin. It provides the basic requirements for bacteria to grow.
Basic nutrient media
BAP and Chocolate are examples of what type of media?
What type of media is formulated to meet the requirements of fastidious pathogens - are essentially basic nutrient media w/ extra nutrients added such as blood, serum, and egg.
Enriched media
What media contains antibacterial substances that inhibit or kill all but a few types of bacteria?
Selective Media
MAC, CNA, and Mannitol Salt are examples of what type of media?
Selective media
MAC, CNA, and Mannitol Salt are examples of what type of media?
Selective media
What type of media allows bacteria to be differentiated into groups by biochemical reactions on the medium?
Differential media
MAC, Mannitol Salt and BAP are examples of what type of media?
What media is used to differentiate Staph aureus?
Mannitol Salt
What type of media are liquids that either contain nutrients that encourage the growth of desired bacteria or contain inhibitory substances that suppress competition?
Enrichment Broth
Selenite Broth and Tryptic Soy Broth are examples of what type of media?
enrichment broth
What info is recorded on day one of record keeping?
Source, what plates streaked, and direct gram smear results.
What info is recorded on day 2 of record keeping?
what plates showed growth, colony morphology, presumptive id (w/ hemolysis), and set up confirmatories and sensitivity testing
What info is recorded on day 3 of record keeping?
Positive ID and sensitivity results
Why do we do dilution streaking?
To obtain isolated colonies of pure growth
4 stains used in Gram staining
Crystal Violet, Gram's Iodine, Acetone Alcohol, Safranin
What shows a positive reaction to a Catalase test, Staph or Strep?
What is Catalase +, but Coagulase and mannitol -?
Staph epi
What is Catalase, Coagulase, and Mannitol +?
Staph aureus
Positive result for Catalase test?
Positive result for Coagulase test?
clumping seen in 5-20 seconds
What is added to bacteria in performing a Catalase test?
Hydrogen Peroxide
What is a positive reaction for a Mannitol Salt Agar test?
Color change in the agar
On which media is sensitivity testing done?
Mueller Hinton, except for Strep which is done on BAP
Positive result for Bacitracin test? Positive result indicates what?
Any area of growth around disc - Beta-Strep Group A
Positive result of Optichin test? Positive for what?
Area of growth greater than 14mm is positive. Strep Pneumoniae.
How to read test result for NaCl test?
+ media turns yellow, - remains purple
How to read test results for Bile Esculin?
+ media turns black, - remains tan
What other organism is needed to perform CAMP test other than Strep?
Staph Aureus
How to read CAMP test results?
+ if there is an arrowhead area of growth, - if no arrowhead