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7 Cards in this Set
- Front
- Back
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What are the universal considerations of cell culture? |
• Aseptic technique; • Temperature [37°C for mammalian cells (and their pathogens…), lower for insect cells, may be very high for some archaea]; • Atmosphere [Carbon dioxide? Oxygen? Other gases?]; • Nutrients [solid or liquid media (tryptone, yeast extract, salts)]; • SAFETY |
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What did the research of Alexis Carrel show? |
1913: Alexis Carrel shows that cells can grow for long periods in culture provided they are fed regularly under aseptic conditions. |
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What are the issues with Alexis Carrel's findings? |
• He published work on his chick embryonic fibroblast cell line when it had been growing for 120 days. It was later reported to have been kept growing for 34 years. >>> • Malignant transformation? • Accidental contamination with fresh cells? • Something worse….. |
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list the types of Organotypic cell cultures |
1. Primary cell cultures 2. Tissue slices 3. Embryonic stem cell organoids |
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3 R's of cell culture |
Reduce, Refine, Replace |
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What are the uses of cell culture? |
# 3 R's - Reduce, Refine, Replace # Regenerative medicine? |
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What are the 2 commonly used yeast biological models? |
1. Schizosaccharomyces pombe (Fission yeast); 2. Saccharomyces Cerevisiae (budding yeast). |
