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28 Cards in this Set
- Front
- Back
- 3rd side (hint)
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What shape are Bacillus Bacteria? |
Rod shaped |
Shape |
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What shape are Cocci Bacteria? |
Spherical |
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What shape are Spirillum Bacteria? |
Corkscrew shaped |
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What is Gram Staining used for? |
In order to distinguish between Gram Positive and Negative bacteria |
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What two layers do Gram Positive bacteria possess? |
Peptidoglycan layer and the membrane |
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What two layers do Gram Positive bacteria possess? |
Peptidoglycan layer and the membrane |
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What three layers do Gram Negative bacteria possess? |
Peptidoglycan layer, membrane and lipopolysaccharide layer |
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Name all stages involved in the growth of bacteria |
LAG, LOG, stationary and death |
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Name all stages involved in the growth of bacteria |
LAG, LOG, stationary and death |
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What 4 things are necessary for bacterial growth? |
Nutrients Temperature pH Oxygen |
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Define the following terms: Total cell count Viable cell count |
Total- living and dead bacteria Viable- living only |
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Define the following terms: Total cell count Viable cell count |
Total- living and dead bacteria Viable- living only |
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Viable count can be made via Dilution Plating. What is dilution plating? |
A 1cm sample is streaked onto an agar plate and left for 2 days in an incubator. Colonies are then counted and multiplied by the dilution factor. |
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Define the following terms: Total cell count Viable cell count |
Total- living and dead bacteria Viable- living only |
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Viable count can be made via Dilution Plating. What is dilution plating? |
A 1cm sample is streaked onto an agar plate and left for 2 days in an incubator. Colonies are then counted and multiplied by the dilution factor. |
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Name some of the steps involved in Batch Fermentation. |
-pure culture added in suitable conditions. -vessel must be sterile and openings protected from contamination. -forced aeration needed for max growth. -temperature must be monitored |
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Define the following terms: Total cell count Viable cell count |
Total- living and dead bacteria Viable- living only |
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Viable count can be made via Dilution Plating. What is dilution plating? |
A 1cm sample is streaked onto an agar plate and left for 2 days in an incubator. Colonies are then counted and multiplied by the dilution factor. |
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Name some of the steps involved in Batch Fermentation. |
-pure culture added in suitable conditions. -vessel must be sterile and openings protected from contamination. -forced aeration needed for max growth. -temperature must be monitored |
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What organism produces Penicillin? |
Penicillium Notatum |
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When and why is Penicillin produced? |
When nutrients begin to deplete (approx 30 mins) in order to destroy organisms competing for these nutrients. |
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Do the following grow better in the presence or absence of oxygen?
Obligate Aerobes Obligate Anaerobes Facultative Anaerobes |
Obligate Aerobes - presence Obligate Anaerobes - absence Facultative Anaerobes - both |
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Do the following grow better in the presence or absence of oxygen?
Obligate Aerobes Obligate Anaerobes Facultative Anaerobes |
Obligate Aerobes - presence Obligate Anaerobes - absence Facultative Anaerobes - both |
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Name the bacteria who's optimum temperature for growth is:
4 degrees 39 degrees 60 degrees 88 degrees |
4 - Psychrophile 39 - Mesophile 60 - Thermophile 88 - Hydrothermophile |
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What colour do Gram Positive bacteria turn? |
Purple |
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What colour do Gram Positive bacteria turn? |
Purple |
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What colour do Gram Negative bacteria turn? |
Red |
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Finish the following sentence:
Penicillin is described as a secondary .............. |
Metabolite |
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