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14 Cards in this Set
- Front
- Back
Why are automated counters better than manual counters?
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1. More cells are counted
2. Coefficient of variance are better in automated 3. Methods 4. Smaller amount of blood is required. 5. Faster |
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How many cells can be counted in an automated counter?
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Usually <8,000-20,000 cells can be counted in ≤1min
(depends on analyser/instrument used) |
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Which automated counter was present in the 1950s?
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Coulter Single channel analyser
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Which automated WCC developed in the 1960s?
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Multi channel analysers, two brands entered the market. Technicon,SMA joined Coulter.
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Which automated WCC developed in the 1970s?
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3 cell differential analysers were introduced with two models Coulter S and Technicon H6000
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Describe the developments of WCC differential - and then 1990s and 2000s
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5 cell differential analysers were introduced in the 1990s with Coulter StkS, Technicon H*1, H*3
6 cell differential (nRBC) 6 cell + flow cytometry |
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Name the '6 cell differential (nRBC)' counter
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Sysmex
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6 cell + flow cytometry automated counters
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CELL-DYN
Advia (Technicon) |
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Advantages of Automated Cell Counting
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Precision
Volume of sample required Operator safety Accuracy Speed Economical use of reagents Parameters/results available |
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What are the most common errors associated with
haematology analysers? |
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What would you do if you were performing an FBE on a
haematology analyser and it gave you a ‘cellular events fl / l ’ flag/alert’? |
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Flow cytometry can be used to label all of the different
cell types found in blood. True or false? |
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Give at least 5 examples where flow cytometry analysis
would be useful for making a diagnosis. |
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Using diagrams and short notes describe in detail a
haematology analyser that is capable of performing an automated differential count and full blood count profile. Use the following headings to structure your response: • Manufacturer and model • Operating principles • Characteristics and capabilities • Advantages/disadvantages and any other information peculiar to this instrument • Calibration and quality control. (15 marks) |
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