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14 Cards in this Set
- Front
- Back
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Normal Values of WBC in dogs and cats
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Dogs - 6.0-17.0 10mm3
Cats - 5.5-19.5 10mm3 |
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4 Purposes of blood smears
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-Classify WBC count
-differentiate the % of each of the WBC's -evaluate platelets -evaluate morphology |
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What does an acceptable blood smear look like?
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Rainbow in critical area
Feathered edge No waves smear should not go to edge of slide |
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How do you make a blood smear?
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1) Place drop of well-mixed EDTA blood on slide
2) Hold spreader slide at angle 3)Push spreader slide back into blood, allowing blood to spread evenly 4)Spreader slide is immediately pushed forward across the stationary slide with a smooth rapid motion 5)Pull it up and away 6) Allow slide to air dry |
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Sources of error when making a blood smear
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-too much blood
-not enough blood -blowing on it to dry -debris on slide -angle of spreader |
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Effect of size of blood drop on your slide
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Larger - longer and thicker smear
Smaller - shorter and thinner smear |
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Names of stains and the order you use them
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1)Methyl alchohol - fixative
2)Eosin -pink 3)Thiazine-nucleus - purple |
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Process of staining
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Let slide dry
Dunk into alcohol 5-6 times, drip off excess Dunk into Eosin 5-7x, drip off excess Dunk into Thiazin 5-6x, drip off excess Rinse off with distilled water, let dry |
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Which stain is needed to increase blue and red colors?
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Thiazine
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How to evaluate blood smears
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10x - scan - look for HW, quality of stain
40x - WBC average 100x - differential |
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How do you get an average of WBC/HPF
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40x
look at 6 fields, count total and divide by 6 |
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How do perform a differential count
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Oil immersion - count each individual WBC
Record types - 100 total cells, record as average |
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Calculate absolulte values
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% WBC type (dec. form)xtotal WBC count
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Procedure for using a hemocytometer to get WBC count
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EDTA mixed sample
blood mixed with 3% acetic acid (10 mins) Charge both sides of hemacytomer Count # of cells in grids under 10x Add totals of both sides together, divide by 2 and multiply that by 50 |
