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13 Cards in this Set
- Front
- Back
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Virus isolation
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release virus from tissue sample, culture on appropriate cell line, observe for cytopathic effect
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Direct Fluorescent Ab test
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antiviral Ab labeled with fluorescein isothiocyanate
Ab applied directly to frozen tissue, smears, cell culture. fluorescence under UV light=presence of viral Ag |
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Indirect fluorescent Ab test
sames FA |
same as FA but 2-step procedure
1st Ab unlabeleds, 2nd Ab labeld with FITC |
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Indirect fluroescent characterisitc advantages
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signal amplificaiton
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Hemagglutination inhibition
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some viruses hemagglutinate red blood cells (HA test)
Presence of AB inhibits hemagglutination |
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Hemagglutination Inhibition
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Some viruses hemagllutinate red blood cells ( HA)
presence of antibody inhibits hemagglutination |
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Hemagglutination inhibition interpretation
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presence of hemagglutination = presence of antibody
absence of hemagllutination = presence of antibody |
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Serum neutralization principle
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Antibody in serum binds to virus. Ab-bound virus unable to infect cells.
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Serum neutralization interpretation
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absence of cpe = presence of Ab
Titer = highest dilution preventing cpe, if all cells destroyed, no Ag present |
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Enzyme linked immunosorbent Assay
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color reaction if AB bound to plate
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Agar gel immunodiffusion
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line of precipitation formed with positive Ab.
AG in center well |
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Egg Inoculation
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Chorioallantoic membrane (CAM
Yolk sac allantoic sac intravenous |
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negative contrast EM
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sample homogenized
debris removed, virus pelleted stained with phosphotungstic acid |
